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THE REDUCING SUGARS CONTENT AND Β-
GLYCOSIDES ACTIVITY IN NONNUCLEAR
CHLOROPHYLL MUTANTS OF SUNFLOWER
03.00.00 Biological sciences
Description
In the Southern Federal University on the genetic basis
of sunflower inbred line 3629, a collection of plastid
mutants with varying degrees of chlorophyll
deficiency was created by inducing Nnitrosomethylurea.
Chlorophyll content was associated
with their photosynthetic activity. It was found that the
lower the chlorophylls content in mutant plastids, the
lower the sugar concentration in plant tissues. For
example, during the entire period of plant growth for
whites (1.0-3.0 % chlorophylls a+b from control) var-
10, var-17 and yellows (6.0-9.5 % chlorophylls a+b
from control) var-29, var-33 leaf areas of variegated
mutants depending on the development phase and the
content of green pigments are characterized by a low
(2-7 fold) sugar content. Yellow-green (75.5%
chlorophylls a + b from control) leaves of en:chlorina-
7 contain a higher level of carbohydrates, although it is
1.5-2 fold lower than at line 3629. Monosaccharides
are products of hydrolytic reactions catalyzed by β-
glycosidases. It was shown, that the activity level of β-
galactosidase and β-glucosidase exceeded the
corresponding indicators of the control at 1.5-2 and 2-7
fold, respectively. Similarly to enzymes from the
water-soluble fraction, membrane-bound β-
glycosidases also showed increased activity in the
leaves of the investigated mutants, compared to the
control green plants of 3629. Consequently, the
activity of β-glycosidases increases dramatically in
leaf tissues with deficiency of photosynthetic. Thus,
chlorophyll mutations can lead to a change in the
expression of nuclear genes, resulting in a significant
increase in the activity of β-glycosidases in the mutant organelles themselves
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