name
Kovalev Victor Savelyevich
Scholastic degree
•
Academic rank
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Honorary rank
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Organization, job position
• All-Russian Research Institute of Rice
заместитель директора по научной работе
Research interests
селекция риса, физиология риса, биотехнология, сортовая агротехника риса.
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TOP5 co-authors
Articles count: 6
Сформировать список работ, опубликованных в Научном журнале КубГАУ
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APPROBATION OF MULTIPLEX SSRANALYSIS FOR DNA-FINGERPRINTS OF RICE VARIETIES
Description
Results of testing of multiplex sets SSR-markers for genotyping of rice varieties are presented in the article. Two sets of SSR-markers were formed: 1: RM1+ RM11+ RM70+RM122; 2: RM164+RM167+RM168. The optimal combination of DNA markers in the multiplex sets and PCR conditions allowed obtaining accurate, easily interpretable results when performing fragment analysis on automated genetic analyzer ABIprism3130. Using multiplex sets, genotyping was performed for several varieties of rice: domestic breeding and one variety – IR36 from the breeding of IRRI (Manila, Philippines). For all the studied varieties specific SSR-fingerprints were obtained. RM 168 marker showed in domestic varieties a low level of polymorphism - one allele of 97 bp. However, at the same time, the variety IR-36, showed a second type of allele 107 bp. In addition, the loci of RM1, RM11, RM167 and RM164 have unique alleles in this variety. It is consistent with significant genetic differences of these varieties and the rest of the varieties in studied sample. The proposed SSR multiplexes are promising for use in DNA certification of rice varieties and assessment of genetic diversity
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EFFECT OF "REASIL UNIVERSAL" MULTICOMPONENT FERTILIZER ON RICE YIELD
06.00.00 Agricultural sciences
Description
The efficacy of using "Reasil Universal" multicomponent fertilizers on the rice crops. It is found that the greatest effect for seed treatment before sowing and foliar feeding of plants was reached at a dose of 300 ml/t or ml/ha. This gives better grain yield of rice, respectively, 5, 43 and 5, 79 kg / ha
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ДНК-ПАСПОРТИЗАЦИЯ СОВРЕМЕННЫХ РОССИЙСКИХ СОРТОВ РИСА С ПРИМЕНЕНИЕМ SSR-МАРКЕРОВ
Description
In the presented study, we have performed genotyping of modern Russian rice cultivars using microsatellite DNA-markers. The markers showed different level of allelic polymorphism: from 2 to 8 alleles per locus. For all studied cultivars,unique DNA-fingerprints were obtained
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Description
Estimation of blast resistance of rice breeding lines, carrying resistance genes Pi-b, Pi-z and Pi-40 has been done. For phytopatological test isolates of pathogen from Krasnodar territory were used. As the results of the study Pi-40 gene showed maximal resistance to blast isolates. On the other part, rice line, carrying both genes – Pib and Pi-z showed significantly higher level of resistance with compare to standart variety Khazar
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06.00.00 Agricultural sciences
Description
Multiplex DNA-marker set for PCR identification for rice blast resistance genes Pi-40 and Pi-b was developed in this study. Optimal primers combinations and PCR conditions allows to identify both abovementioned genes in the single PCR
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06.00.00 Agricultural sciences
Description
The results of development of rice breeding lines, carrying the wide range resistance gene to rice blast disease - Pi-40. For identification of the dominant allele of the gene the DNA - marker analysis was used. With co-dominant DNA markers plants from inbred populations that carry a dominant allele of this gene in the homozygous state were selected
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