name
Skoblikov Nikolay Eduardovich
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Academic rank
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Honorary rank
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Organization, job position
• North-Caucasus Research Institute of Animal
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Articles count: 7
Сформировать список работ, опубликованных в Научном журнале КубГАУ
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ISOLATION AND SELECTION OF THE NON TRANSDUCING E. COLI BACTERIOPHAGES FOR ANTI-COLIBACILLOSIS DRUGS
Description
The isolation of E.coli phages from samples of natural and waste water obtained during expeditions in the different regions of Russian Federation was carried out. The obtained phages (286 isolates) were tested for their ability to lyse the pathogenic strains of E. coli – pathogenic agents of pig colibacteriosis in Krasnodar region. The study was conducted of their ability to phage transduction, the molecular-genetic characterization and biotechnological parameters of selected bacteriophages. For first experimental design of veterinary drugs was selected 5 coliphages having no ability of plasmids transduction. It has been shown that all the investigated phages are representatives of T4-type phages of family Myoviridae. The reported study was partially supported by RFBR, research projects No. 08-04-99111, 09-04-10132, 16-44- 230855
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THE APPLICATION OF THE BACTERIOPHAGES TO COMBAT COLIBACILLOSIS AND CAMPYLOBACTERIOSIS IN POULTRY
Description
This work considers the needs and methods of phage therapy of two bacterial infections in poultry. The review examines the use of bacteriophages, from the moment of their discovery to the present day. We discuss the relevance of such diseases as compilobacteriosis and colibacteriosis transmitted from birds to humans, we show how to use bacteriophages and their efficacy in models of adult birds and chickens, including information provided on the pilot study of phage therapy at a poultry farm. The reported study was partially supported by RFBR, research projects No. 16-44-230855 and No. 13-04- 00991 and № 16-44-230855-р_а
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06.00.00 Agricultural sciences
Description
Studied the investigation of the therapeutic effect of experimental mixture of four non-transducing E. coli bacteriophages and it combinations with probiotic, designed on the basis of the endemic Lactobacillus paracasei strain, previously isolated from pigs in this age of the local population in the state, the survival rate and laboratory parameters of pigs with post-weaning diarrhea. The most influence on the correction of E. coli dynamics has a combined oral administration of phage medicine and probiotic preparation established. The most influence on the dynamics of titer phages has not only the combined using (both oral and intradermal) of phage medicine with the probiotic preparation, but also an isolated ingestion of phage mixture
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CONSTRUCTION OF MUTANTS OF BACTERIOPHAGE T4 WITH REDUCED ANTIGENICITY
Description
A method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
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06.00.00 Agricultural sciences
Description
The influence of four experimental phage preparations, designed based on non-transducing E. coli bacteriophages on pigs with post-weaning diarrhea studied. The influence of these phages in depending by methods of its applying (per os and intra cutis) on coli-titer and coli-phages titer studied. It is established, that the best survival rate and the clinical condition showed animals treated with the E. coli phage T4-type with lab. number «8» (till 5,52 lg CFU/g at 37-days age) in parallel with an increase in titer of phages (till 5,73 lg CFU/g at 40-days age). Intradermal (intra cutis) method of applying of experimental phage preparations (constructed on basis of phage “6” and phage “8”) showed the some positive effects on correction of negative dynamics of coli-phages titer in control group
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