name
Koshchayev Andrey Georgiyevich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
• Kuban State Agrarian University
Research interests
Web site url
—
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Current rating (overall rating of articles)
0
TOP5 co-authors
Articles count: 41
Сформировать список работ, опубликованных в Научном журнале КубГАУ
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EXPERIENCE AND PROSPECTS OF SEXING SEMEN FOR CATTLE OF DAIRY COWS IN THE KUBAN REGION
06.00.00 Agricultural sciences
DescriptionThe article is an analysis of the results of three years of work with sexing sperm in one of the two farms of the Kuban region which have implemented this tech-nology
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MEAT PRODUCTIVITY OF BULL CALVES UNDER INTENSIVE REARING
DescriptionThe article presents a comparative assessment of the meat productivity of young cattle of the two most common intensive meat breeds in the Russian Federation (Aberdeen Angus and Hereford) in comparison with the most numerous native in the South of the country domestic breed, which is Kalmyk. Intensive rearing from 9 to 18 months of age was carried out in the conditions of industrial fattening complex on rations not containing juicy forages. In the process of 273-day intensive rearing, in bulls of the tested breeds average daily live weight gains from 1455 to 1526 grams were achieved. During this period, an absolute increase was obtained in Aberdeen-Angus bulls-416.6 kg, in peers of Hereford and Kalmyk breeds-413.6 and 397.2 kg. The highest pre-slaughter live weight and slaughter rates were noted in Aberdeen-Angus bulls, having a yield of a pair carcass of 59.4 % and a slaughter yield of 62.8 %. The meat of Aberdeen-Angus bulls has the highest content of fat and dry matter, but they have less protein, lower pH and moisture binding capacity and a higher loss of meat juice during cooking, compared with the bulls of the Kalmyk breed. It was confirmed that the coefficients of transformation of protein and energy into products are closely related to the intensity of growth and weight of the carcass. The most effective of these indicators were in bulls of Aberdeen-Angus, then Hereford and Kalmyk breeds. A similar pattern between the breeds was manifested in terms of profitability and cost recovery
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06.02.00 Veterinary and Husbandry
DescriptionIn this work, we have found shortened particle forms of the filamentous bacteriophage Pf, when the lysis of the Pseudomonas aeruginosa PAO1 strain was induced by extracts of bison feces to study the intestinal bacteriophages of this animal. The sizes of the shortened particles from 250 to 950 nm and their thickness 17 nm were determined. The particles had a slightly pointed one end and sometimes a flag on the other extended end. The particles were formed in the process lysis of this strain by other bacteriophages. These lysates also contained a high concentration of vesicles from the pseudomonad membrane. The article discusses the mechanisms of prophage induction in the Pseudomonas aeruginosa PAO1 strain due to phage-phage induction and substances of the investigated extract
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MODELING REGIONAL SYSTEMS OF BREEDING PIGS
06.00.00 Agricultural sciences
DescriptionThe article contains the experience of the development of the methodology and the computer program for calculation of regional and local systems of pigs hybridization at the example of the Rostov region (Russia). Crossing the GP lines for F1 should be organized in multiplier farm, which may be separate farms and to be part of large commercial farms. For the production of F1 in a multiplier farm, we must breed a purebred specialized paternal and a maternal line, selected on the effect of combining ability. For the successful functioning of the system of hybridization, it is necessary to build a genetic pyramid, including breeding and genetic centers (nucleus farm), multiplier farm, reproducing the baseline. The article gives a detailed calculation of sow population of levels of P, GP, GGP for maternal and paternal breeds of pigs. The program uses user-defined parameters of pigs productivity, of the simulated population, and the parameters characterizing the intensity of selection of young animals. To ensure annual production of 1,822 million pigs in the Rostov region it is necessary to provide the availability of brood stock in the amount of 89 thousand heads, 6 800 heads in the structure of grandparent flocks (GP), 730 heads in the structure of the Grand-Grand-parent stock (GGP), excluding sows second maternal and paternal breeds
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CORRECTION OF DYSBIOTIC DISORDERS IN BEES BY USING PROBIOTIC MEDICATIONS
DescriptionThis article provides an overview of the need of the use of probiotic supplements and medications in the diets of insects, in particular bees and their families. We present a list of both Russian and foreign probiotics and their complexes, which are used in beekeeping for the treatment and prevention of various diseases, are shown. Their effectiveness is shown not only for the prevention and treatment of microbial disorders in the gastrointestinal tract of bees due to the manifestation of antagonistic properties in relation to opportunistic and pathogenic microflora, but also the possibility of their use as tools that can increase the immune status, the safety of bee families, and life expectancy. The article shows data on the positive effect of probiotics on the physiological and biochemical parameters of honeybees, activation of metabolic processes in the formation of a fat body, growth of strength and weight of larvae, development of pharyngeal glands, etc.
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CORRECTION OF HOMEOSTASIS OF THE ORGANISM OF LARGE CATTLE AT THE NODULAR DERMATITIS
06.02.00 Veterinary and Husbandry
DescriptionWe have studied biochemical changes in the body of cattle, sick with nodular dermatitis in the initial stage of the clinical manifestation of the disease. It is proved that in case of disease in animals there is a significant increase in the level of creatinine up to 295.5 mg%. The excess of the norm by 82.1%. In treatment, this indicator was significantly reduced (189.2 mg%), but did not reach the upper limits of the norm (162.4 mg%). In the blood serum of patients, the carotene content was reduced to 0.09 mg%, which was restored to normal three days after the start of treatment (1.0 mg%). The glucose level at the beginning of the disease was 18% below normal and reached the lower limit of the norm 8 hours after the start of treatment. When studying the activity of CKK, it was found that the activity of the enzyme in animals before the introduction of a 5% solution of sodium hydrogencarbonate is 82% higher than the upper limit of the norm. After the treatment, this indicator returned to normal. The inorganic phosphorus index did not reach the physiological norm (2.5 mmol / l) before and after treatment, and remained 11.6% higher (2.9 mmol / l), which indicates a violation of mineral metabolism in sick animals. The level of reserve alkalinity began to recover after 8 hours of observation and reached a minimum limit of 48.91 vol. % CO2. During the entire period of the experimental studies, before and after the administration of the drug, there were no significant changes in the biochemical parameters of total protein, calcium, vitamin E and urea. The nature of the established changes varied within the limits of physiological norms. The use of a 5% solution of sodium hydrogen carbonate in nodular dermatitis increases the effectiveness of symptomatic treatment, reduces the likelihood of complications and stimulates the production of specific antibodies against viruses and bacteria. We have recommended intravenous injection of 5% sodium hydrogencarbonate solution to sick animals at the rate of 1 ml per 1 kg of live weight of the animal three times with an interval of 3 days
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DESIGN OF THERAPEUTIC PHAGE COCKTAILS BASED ON T4-TYPE BACTERIOPHAGES: ADVANTAGES AND DISADVANTAGES
DescriptionIn the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy
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CONSTRUCTION OF MUTANTS OF BACTERIOPHAGE T4 WITH REDUCED ANTIGENICITY
DescriptionA method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
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Description
The work was done in the research laboratory of the department of Biotechnology, Biochemistry and Biophysics of Kuban State Agrarian University, the aim of which was to select the most appropriate nutrient medium for maximum growth of lactic acid microorganism growth. The object of the study was its own microflora of gastrointestinal tract of quails ‒ Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius. As a nutrient substrate there were used 4 of the nutrient media: the medium for lactic acid bacteria (city Uglich), the melasse-autolysis medium, the glucose-peptone medium and the MRS. During the cultivation of the microflora of the variable parameters were the time and temperature of cultivation. During the cultivation of microflora the time and the temperature of growing were the variable parameters. In the process of growing there was carried out the analysis of dynamics of consumption of reducing substances and titers of microorganisms. According to the results of growing of microbial cultures there was revealed the active consumption of carbon substrate in used variants of nutrient media, and it was found that there was observed the maximum of cells to 24 h of cultivation. On the basis of carried out results of cultivation on different media and at different parameters there was determined that the most titer of cells was reached to 24 h at the temperature 38,0 °С on the melasse-autolysis medium. So, the melasse-autolysis medium can be recommended in production conditions as the cheaper substrate at the further development of biological preparations for poultry breeding
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ANIMAL HEALTH - CRITICAL FACTORS OF THE LIVESTOCK
DescriptionThe article provides an overview of materials of conferences on problems and prospects of the industry of cattle breeding