name
Salfetnikov Anatoliy Alekseevich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
• Kuban State Agrarian University
Research interests
Web site url
—
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Current rating (overall rating of articles)
0
TOP5 co-authors
Articles count: 17
Сформировать список работ, опубликованных в Научном журнале КубГАУ
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ESTIMATION OF INITIAL MATERIAL FOR BREEDING OF WINTER BARLEY IN KUBSAU
06.00.00 Agricultural sciences
DescriptionResults of three-years of examining the collection material of winter barley of different ecological-geographic origin are brought in this article. We have also allocated sources of high grain productivity, winter hardiness and precocity for further use in breeding programs
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SPECIFICITY OF TRILON B INFLUENCE ON THE GERMINATION OF SEEDS OF VARIETIES OF WINTER BARLEY
DescriptionThe problem of increasing resistance of winter barley to adverse conditions of winter is the most problem in the selection area of this crop. The main component of this complex trait is the frost resistance. Laboratory methods of assessment of frost resistance are important for breeding of winter barley. These methods can effectively replace more costly method of direct freezing in the freezers. Magnesium cations (Mg++) are essential components of the protein-synthesizing system of germinating seeds. Magnesium cations determine the stability and translational activity of mRNA and rRNA. Trilon B is usually used for in vitro banding of magnesium cations in area of biochemistry of the nucleic acids. It was very interesting to verify the Trilon B influence to seed germination of winter barley varieties by experiment. Experiments showed: actinomycin D – the inhibitor of RNA synthesis – didn’t show varieties specific effect to seed growth of winter barley, while Trilon B had a varieties specific impact to length coleoptiles and roots. Research was carried out on etiolated seedlings at temperatures from 22 to 28oC, and different concentrations of Trilon B - 1,6×10-3М, 2,4×10-3М and 3,2×10-3М. The roots of 3-days old seedlings were more sensitive to Trilon B influence. It is shown the varieties of Russian origin were naturally reacted to Trilon B: the higher the frost resistance of variety, the more resistant variety to the action of various concentrations of Trilon B at different temperatures. The most optimum concentration was 3,2 × 10-3М at 22-25oC. Varieties by foreign origin deviated from this pattern. It is interesting to research the genetic essence of these differences. It will be helpful for optimization of varieties differentiation conditions to create a laboratory method of estimate of frost resistance of winter barley. The new method will be most efficient compared the direct freezing method
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STABILIZATION OF MRNA CEREALS IN VITRO UNDER THE SILICON INFLUENCE
DescriptionThe differential stability of mRNA is an important mechanism for posttranscriptional regulation of gene expression in eukaryotes. Messenger RNA stability is controlled by specific genes and growth conditions. The review examines the theoretical possibility of mRNA stabilization in vivo as a consequence of replacing carbon atoms (C) or phosphorus (P), composed of nucleic acid on the silicon (Si) atom. During isolation of poly-(A)+mRNA from plant tissues by the two-cycle affinity chromatography on poly-(U)-Sepharose, regular changes in poly- (A)++mRNA yield were observed. The changes varied both with the plant genotype and growth conditions. Celite treatment of heated and unheated total RNA preparations from developing corn kernels and from green and etiolated wheat seedlings. Messenger RNA that differed in the length of poly- (A)-sequences was used for hybridization. It is evident that a reduction of poly-(A)-length causes alterations in spatial structure of mRNA, and associated proteins and cations Mg++ become accessible to celite absorption. Heating promotes melting of secondary structure, already initiated, and increases the efficiency of mRNA stabilization by celite. Interpreting the facts interact celite with mRNA in vitro and stabilization of mRNA in vivo by cycloheximide with a modern point of view can be considered with the position research of the phenomenon of RNA interference
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Description
Due to the wide spread of winter barley varieties with different Phytopathologic characteristics, a serious threat to the crop is dwarf rust caused by a pathogenic fungi of the Puccinia. The article presents the results of phytosanitary studies conducted in different agroclimatic zones of the North Caucasus region for the period from 2013 to 2015. Electronic maps of levels of development and distribution of dwarf rust are based on phytosanitary inspection. On the experimental field called «Kuban» as well as rust nursery of VNIIBZR we have performed an immunological evaluation of the sustainability of winter barley accessions to the pathogen of the dwarf rust on natural and artificial infectious backgrounds in the period from 2013 to 2015. The article reflects the objective characteristics of varieties and lines of winter barley with a relative stability to the North Caucasian population Puccinia hordei. The most promising varieties and lines of winter barley of the KubGAU breeding were selected during operation. These varieties and lines are highly resistant to rust and dwarf, they also have other valuable features. The article presents information on the best yield varieties and lines of winter barley as well as on gross yield and sowing area
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CYCLICAL EFFECTS OF ACTINOMYCIN D ON THE BARLEY COLEOPTILES GROWTH
DescriptionIn cell biology, actinomycin D is shown to have the ability to inhibit transcription. Actinomycin D does this by binding DNA at the transcription initiation complex and preventing elongation of RNA chain by RNA polymerase. When soaking the seeds in a solution of actinomycin D, antibiotic blocks RNA synthesis and seed germination occurs at the expense of long-living RNA, available in the mature seed. In the article we present experimental data indicating that as storage seeds of winter barley are changing the action actinomycin D on the growth of coleoptiles. It is shown that actinomycin D at a concentration of 40 µg/ml in October reduced the growth, in December it was not authentically, and in February it was paradoxical amplified growing coleoptiles. We suggest the part of the actinomycin D fastened with growth inhibitor coleoptiles, whose structure changes during seed storage and increase the affinity of actinomicyn D to the inhibitors. At a concentration of 60 µg/ml actinomycin D effectively reduced of the coleoptiles growth. Suppression of seedlings roots growth was proportional to the concentration of the actinomycin D and exposure time