name
Aniskina Mariya Vladimirovna
Scholastic degree
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Academic rank
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Honorary rank
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Organization, job position
• Kuban State Agrarian University
Research interests
Биотехнология, кормопроизводство
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TOP5 co-authors
Articles count: 4
Сформировать список работ, опубликованных в Научном журнале КубГАУ
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DEVELOPMENT OF OPTIMAL WAY OF OBTAINING A HYDROLYZATE OF LACTIC ACID BACTERIA
Description
The work was carried out in the scientific-research laboratory of the chair of Biotechnology, Biochemistry and Biophysics of Kuban SAU, the aim of which is the search of optimal conditions of obtaining the hydrolyzates of lactic acid bacteria by thermoacid and enzymatic ways. The pure cultures of microflora of gastrointestinal tract of quails − Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius were the object of researches. Under conducting the thermal acid hydrolysis there were used the same correlations of bacterial mass and water as well as procedures of clearing of hydrolyzates. The variable conditions were рН, temperature and time of hydrolysis. When carrying out the enzymatic hydrolysis in this study there was used the enzyme from the group of muramidase lysozyme. The amount of enzymes and time of carried out hydrolysis were the variable conditions under obtaining the enzymatic hydrolyzates. In the process of researches there were studied: qualitative content of total protein, peptides with М.м. < 1500, protein and peptides with M.m. > 1500, aminoacids and as well as GMDP (glucosaminilmuramilpentapeptide). On the basis of carried out experiments there was stated that according to all components of biologically active substances the thermal acid way is more effective than enzymatic. The maximum accumulation of BAA in hydrolyzates by different ways was revealed at destruction of cells Lb. intermedius. Content of GMDP in thermal acid hydrolyzate was higher than in enzymatic on 0,028 g/100 ml (51,8 %)
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Description
The article presents materials about selection and optimization of the nutrient medium for cultivation of Propionibacterium shermanii. We have shown a high positive effect expressed in the intensive growth of microorganisms of Propionibacterium shermanii on optimized medium with the juice from the tomatoes, as well as replacement of glucose on corn extract, which allows concluding that these components have a positive impact on the accumulation of biomass of Propionibacterium shermanii
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Description
The article presents the materials of the study of the features of cultivation of Lactobacillus sp. on different culture medium. We have made a comparison of standard culture medium for cultivation Lactobacillus sp. and determined the most optimal culture medium
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FEATURES OF THE CULTIVATION OF PROPIONIBACTERIUM SHERMANII STRAIN
Description
We have presented materials about nutrient media for growing and industrial the cultivation of Propionibacterium shermanii: Trypticase Yeast Extract Glucose Medium, the culture medium Ellinger and ATCC Medium 33. We have studied pH change and its impact on the biomass accumulation of propionic acid microorganisms. We have also defined the best time of cultivation
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