name
Gvozdetskii Nicholai Alekseevich
Scholastic degree
—
Academic rank
—
Honorary rank
—
Organization, job position
• Stavropol State Agrarian University
Research interests
Ветеринарное акушерство, репродуктивные технологии
Web site url
—
Current rating (overall rating of articles)
0
TOP5 co-authors
—Articles count: 1
Сформировать список работ, опубликованных в Научном журнале КубГАУ
-
Description
The article presents the use of intensive methods of diet herd reproduction that will increase the productivity and profitability of the sheep industry. One of such methods is in vitro fertilization (IVF). For the success of in vitro fertilization procedure, an important requirement is the availability of highquality nutrient media, which help to preserve the genetic material and contribute to the further development of the zygote. The main requirement to the media for sperm is the ability of media not to cause their agglutination. The aim of our work was to search for new ways to reduce the agglutination of spermatozoa in the preparation of freshly prepared sperm in the process of production of embryos in vitro. To eliminate the agglutination of spermatozoa in the semen preparation stage, we used GCY medium, followed by demolition of seed in SOFw environment, which has resulted in a significant (almost 15 times!) reduce of the number of bound sperm. In our opinion, a decrease in agglutination in GCY buffer was connected with a specific influence of the constituent components of sperm. Conclusion: Thus, our method of preparation of freshly prepared sperm for in vitro fertilization allows a sharp decline in sperm agglutination, which will improve the fertility of eggs during the production of embryos in vitro sheep
pdf 144.680kb
doc 144.680kb