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Machneva Nadezhda Leonidovna
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• Kuban State Agrarian University
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Articles count: 2
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INTENSIFICATION OF CULTIVATION OF CHLORELLA WITH THE USE OF IRON NANOPARTICLES
Description
The influence of reduced iron nanoparticles and its oxide Fe2O3 on the growth rate of Chlorella vulgaris IFR # S-111 added to the Tamiya nutrient medium is studied. In the range of concentration of additives 0-0,1 g/l (gram per litre) an oxide inhibited the growth of microalga already when its content in the environment 6,25*10-3 g/l. With the increase of reduced iron concentration in the first twenty-four hours in the whole range the growth of the cell concentration of microalga was observed. With a maximum iron concentration of 0,1 g/l the density of a chlorella exceeded the control for 70%, and in 48 hours for 150%. The microscopy hasn't shown morphological changes of a chlorella cells with addition of the nanoparticles to nutrient medium. Accelerated reproduction of the microalga cells became the result of the intensification of the photosynthetic processes, as indicated by the nature of the parameters of delayed fluorescence (DF) of chlorophyll and shapes of the induction curves. The maximum level of DF in all experimental samples after 24 hours of cultivation was significantly higher than the control, and photosynthetic activity increased with increasing concentration of nanoparticles. In 48 hours the maximum activity was observed at concentration of nano iron 0,0125 mg/l, then decreased. It shows that with an intensive growth of a chlorella the potential of nutrient medium was quicker exhausted that led to decrease in intensity of photosynthetic processes
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Description
The work was done in the research laboratory of the department of Biotechnology, Biochemistry and Biophysics of Kuban State Agrarian University, the aim of which was to select the most appropriate nutrient medium for maximum growth of lactic acid microorganism growth. The object of the study was its own microflora of gastrointestinal tract of quails ‒ Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius. As a nutrient substrate there were used 4 of the nutrient media: the medium for lactic acid bacteria (city Uglich), the melasse-autolysis medium, the glucose-peptone medium and the MRS. During the cultivation of the microflora of the variable parameters were the time and temperature of cultivation. During the cultivation of microflora the time and the temperature of growing were the variable parameters. In the process of growing there was carried out the analysis of dynamics of consumption of reducing substances and titers of microorganisms. According to the results of growing of microbial cultures there was revealed the active consumption of carbon substrate in used variants of nutrient media, and it was found that there was observed the maximum of cells to 24 h of cultivation. On the basis of carried out results of cultivation on different media and at different parameters there was determined that the most titer of cells was reached to 24 h at the temperature 38,0 °С on the melasse-autolysis medium. So, the melasse-autolysis medium can be recommended in production conditions as the cheaper substrate at the further development of biological preparations for poultry breeding
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