name

Nefedchenko Aleksey Vasiljevich

Scholastic degree

•

Academic rank

—

Honorary rank

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Organization, job position

• Federal state budgetary institution of science Siberian Federal scientific center of agrobiotechnologies of the Russian Academy of Sciences
  Диагностический центр
  старший научный сотрудник

Research interests

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Email

nav-vet@mail.ru

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TOP5 co-authors

• Koshchayev A. G. ¹
• Terentyeva T. E. ¹
• Glotov A. G. ¹
• Glotova T. I. ¹

Articles count: 1

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• pdf  604.625kb doc 604.625kb Views: 293 Date: 29.03.2019
  Nefedchenko A. V. Glotov A. G. Glotova T. I. Terentyeva T. E. Koshchayev A. G.

  ALLELE-SPECIFIC PCR FOR TYPING OMPH-TYPES OF PASTEURELLA MULTOCIDA AND SEARCING FOR NEW APPROACHES TO STUDYING OF PATHOGENICITY OF BACTERIAL ISOLATES

  06.02.00 Veterinary and Husbandry

  Description

  Pasteurella multocida is an important respiratory pathogen of cattle. OmpH Protein is a major protective antigen of bacteria has been well studied in avian strains. In the literature there are no data available for the study of a variety of sequence of this protein among isolates with cattle respiratory pathology. There have been described several genes associated with the virulence of the bacterium in respiratory disease of cattle, but none of the authors compared the frequency of detection of these genes with the pathogenicity for laboratory animals. The aim of our study was the development of allele-specific PCR to determine Omph-types of Pasteurella multocida and the search for new approaches to assess the pathogenicity of isolates of bacteria. Total amount of 83 isolates allocated from the lungs of calves with respiratory pathology was investigated. All isolates belonged to groups A or D (isolates 63 and 20, respectively). Among isolates of capsular serogroup A we revealed 6 types, most propagation types were A1 and A2. All isolates of capsular serogroup D were one omph- type. In 16 out of 23 farms there were identified isolates of only one omph-type, 4 - 2 types, 3 - three types. The frequency of gene hgbb - hemoglobin binding protein correlated with pathogenicity of isolates for white mice. The developed allele-specific PCR along with hgbb gene detection can be used for screening and studying the properties of antigen and circulating pathogenic isolates and selecting a candidate vaccine strains