name
Troshin Leonid Petrovich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
• Kuban State Agrarian University
кафедра виноградарства
зав. кафедрой
Research interests
генетика и селекция растений
Web site url
—
Current rating (overall rating of articles)
0
TOP5 co-authors
Articles count: 170
Сформировать список работ, опубликованных в Научном журнале КубГАУ
-
EXTRACTING DNA WITH USING PEQGOLD PLANT DNA MINI KIT
DescriptionIn the present article, the new method of DNA extraction from fresh and herbarium leafs of grape to their subsequent sequencing was described
-
EXTRACTING DNA BY NUCLEOSPINPLANT 2 COREKIT. COMMENTS
DescriptionThis review article highlights a new method of DNA extraction from herbarium leaves of grapes for use in PCR-analysis
-
EXTRACTION OF DNA FROM LEAVES OF VITIS VINIFERA L.
06.00.00 Agricultural sciences
DescriptionExtraction of the DNA from leaves of genus Vitis is a very difficult task, as a grape has a lot of elements, which make difficult to use it in the genetic manipulation. In that article used the methods of extraction of the DNA with silica (SiO2) particles as the absorbent material for getting better quality DNA
-
06.00.00 Agricultural sciences
DescriptionThe paper reviews the results of the study in in Anapо-Taman zone Krasnodar territory e of influence treatment vines Saperavi growth promoters Immunotsitofit, Krezatcina and HB-101ECO on quality parameters wine
-
INFLUENCE OF THE NEW RACES OF YEASTS ON THE CHEMICAL COMPOSITION OF THE WHITE TABLE FAULTS
DescriptionThe influence is shown for the race of yeasts to the dynamics of the fermentation of grape must, the composition of organic acids and aromatic of components. The races, which ensure obtaining high-quality winemakings material are presented
-
INFLUENCE OF FERTILIZERS ON QUALITY OF CHARDONNAY GRAPES AND WINE MATERIALS
DescriptionThe article presents the results of the fertilizers use effect on dry wine materials produced from the Chardonnay variety, grown in the Anapo-Taman soil and climatic zone of the Krasnodar region, Russia. As a result of wine materials analysis, increase of tartaric acid was noted, with the autumn application of phosphorus-potassium fertilizers and the spring application of ammonium nitrate. At the same time, its optimum content was noted with nitroammophos. Also, the minimum concentration of malic acids was detected by the application of nitroammophos with potassium fertilizers. In its turn, early spring application of ammonium nitrate reduced the concentration of malic acid below the control sample (without fertilizers). It was revealed that the use of mineral fertilizers in this winemaking zone also contributed to a reduction of lactic acid in the must. At the same time, the application of nitroammophos (especially together with other mineral fertilizers), contributed to the accumulation of citric and succinic acids, as well as phenolic compounds. Moreover, the application of phosphate-potassium fertilizers in the autumn contributed to the accumulation, and, consequently, also to the increase in the content of phenolic compounds in the resulting must. In addition, the studied samples of wine materials contained high alcohol concentrations and low concentrations of sugars with volatile acids. At the same time, the maximum amount of alcohol exceeding its content in the control sample was revealed when phosphorus-potassium fertilizers were used together with nitroammophos fertilizers. It is also noted that the application of ammonium nitrate did not affect this indicator. As a result, the use of mineral fertilizers in the studied concentrations made it possible to prepare wine materials that contained a low concentration of sulfur dioxide, which is substantially lower than the harmful concentration for humans. In addition, increased concentrations of hydrogen ions in variants with the application of phosphate-potassium fertilizers have made it possible to reduce the number of iron tanates and the intensity of oxidation-reduction processes
-
INFLUENCE OF FRUITLESS SHOOTS ON QUANTATIVE TRAITS OF GRAPES AND WINE OF SAPERAVI VARIETY
06.00.00 Agricultural sciences
DescriptionIn this study we have shown the influence of fruitless shoots to number of berries in clusters, the mass of 100 berries, average weight of the bunch, yield, sugar content and acid in the juice of the berries, the quality of wine. These indicators have been studied at a ratio of fertile and sterile shoots 1: 1, 3: 1, 6: 1 with a load of bushes 12, 16, 20 fruit-bearing shoots and nutrition area 3 x 1 m2. Control was a natural balance of fruit-bearing and barren shoots. Digital material is processed by one-way analysis of variance experience for each load of bushes, and then the same method of two-experience for all the options. Fragmentation of sterile shoots with load of 12 fruit-bearing shoots per plant decreases, and with loads of 16 and 20 increases the number of fruit-bearing shoots berries in clusters, the mass of 100 berries, average weight of the bunch, yield, and sugar content in berries. For the content of titratable acids we have marked a tendency to increase with a decrease in the number of sterile shoots. The content of phenolic substances on the variants of the experiment was 2.2-2.3 g / dm3, the acidity of 7.3-7.8 g / dm3, alcohol 10,2-10,9% vol. Dry wines tasting score on the options was in the range of 7.4-7.6 points. Significant difference in taste and smell features wines by variants of the experiment was not revealed. Conclusions on the effect of sterile shoots on fruiting vine indicators drawn from the analysis of variance one- and two-factor experiments, in most cases were the same
-
INTRODUCTION OF NEW GRAPES VARIETIES TO THE IN VITRO CULTURE
DescriptionClonal micro-multiplication – is this the basic method of obtaining the qualitative virus-free planting material. The high quality specificity of the explants reaction of the grapes varieties to the composition of nutrient medium requires the individual selection of the medium components for the most successful multiplication in vitro. In the article we present the results of studies on the cultivation of the grapes apexes in vitro the modified medium with the reduced content of macrocells. As a result of conducted investigations we have established that for the grapes varieties of Academic Trubilin, Artemis, Gurman Kraynova, Maria Kallas, Nizina, Petit Verdo and Traminer Black the most effective introduction into the in vitro culture (acclimatization of apexes 80- 100%) occurs on modified nutrient medium to Murasige and Skoog (1962), by being differed in terms of the content macro-elements (mG/l): NH4NO3 – 1237; KNO3 – 1425; MgSO4 · 7N2O – 277,5; KN2PO4 · H2O – 277,5, of the vitamin Of v1 – 10,0 mG/l, nicotinic acid – 4 mG/l. Remaining varieties of Kishmish luchistiy, Preobrajenie, Roshfor K and Yubiley Novocherkasska in the same medium were developed slowly and in a different way
-
INTRODUCTION OF NEW PERSPECTIVE TABLE GRADES OF GRAPES TO IN VITRO CULTURE
06.00.00 Agricultural sciences
DescriptionIn this article we present information on features of microcloning in culture of in vitro of perspective for Eurasia, pink and white-berry table genetic grades of grapes by I. A. Kostrikin, V. N. Kraynov and V. V. Zagorulko. Researches were conducted at an input stage in culture of in vitro of meristems of 0,1-0,2 mm in size. At the majority of grades and forms, we noted good regeneration abilities in the conditions of sterile culture. It is bound to the fact that almost all the studied grades: Anyuta, Bogatyanovsky, the Princess Olga, Preobrazhenie, Anniversary of Novocherkassk, Favor are received from crossing of couple the Mascot × Sultana-ray grape. And only grades called Libya and Lowland which showed lower regeneration properties at stages of proliferation and rooting of shoots are received from crossing the couple of Flamingo × Arkadya and (Talisman x Tomaysky) respectively. At the same time, the grade of Talisman, being one of the parents of the majority of the studied grades, showed stable results at all stages of cultivation
-
VASILY NOSULCHAK - THE NEWEST COMPLEX-RESISTANT TABLE SEEDLESS WHITE GRAPE VARIETY
DescriptionVasily Nosulchak, the newest complex-resistant tableless seedless grape variety, was bred in Greece by Pantelei Zamanidi and Leonid Troshin in 2013 by crossing the Talisman variety with the Yanaky variety. The created variety is a complex inter-species Euro-American-Amur hybrid. The duration of the production period is 146-155 days. The growth of shoots is strong. The degree of grapevine maturing high. Yield is very high. Percentage of fruit-bearing shoots 90. Average weight of bunches 900 g. It is stainable for high winter hardiness, drought resistance and increased resistance to fungal diseases, tolerant to phylloxera. The top of the young shoot is green without pubescence. Young shoots are green, without pubescence. The flower is hermaphroditic. The cluster is large, conical, branching, winged, of medium density. The berry is medium-sized, short elliptical, green-yellow. Peel is thin, strong. The pulp is juicy, with a varietal flavor. The sugar content is high. Rudiments of seeds are soft. The variety is intended for fresh consumption and kishmish production. The table high-yielding seedless variety Vasiliy Nosulchak in terms of winter hardiness, resistance to diseases and pests significantly exceeds all Eurasian varieties of table and kishmish designation. Can be used as a table grapevine for fresh consumption on site and for export, as well as for the production of high-quality dried products. As a winter-hardy variety, it is very promising for cultivation in covered viticulture zones, where table varieties require shelter for the winter. It is of great interest for selection work in breeding frost-resistant, diseases and pests of seedless varieties. To determine the influence of different ecological conditions on the growth, development, quantity and quality of the crop, the variety must be tested on all continents in different ecological and geographical areas of cultivation - in the grapes producing countries of America, Eurasia, Australia, Africa