№ 147(3), March, 2019
Public date: 29.03.2019
Archive of journal: Articles count 13, 41 kb
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RICE VARIETY CALLED LEADER: BIOLOGI-CAL BASIS OF AGROTECHNICAL ELEMENTS
DescriptionRussian rice variety called Leader is widely cultivated in the Kyzylorda region of Kazakhstan. Elite seeds of the variety are produced in the Krasnodar region. Leader possesses field resistance to blast disease. One of the mechanisms for the formation of rice plant resistance to the causative agent of the fungus Pyricularia oryzae Cav is an increased accumulation of silicon (SiO2). Biochemistry studies have shown that pathogen-resistant varieties accumulate up to 19% silicon in flowering scales, and susceptible - less than 14%. The article presents the study materials of rice plants of the rice variety Leader with sowing rates of 700 and 350 seeds per m2 in the field trials. As a result, a correlation has been established between the plant density of the rice variety Leader, the silica content in the flowering scales of the kernels and the damage to the plants by blast disease. Shoots of rice were obtained when wet, so field germination reached 69-70%. In the first variant, the number of sprouting exceeded 490 pcs/m2 with single-stem plants having poorly developed panicles and partially infected by P. oryzae. The yield of rice was 5.75 t/ha. In the second variant, the density of seedlings was 244 pcs/m2. The plants were well developed, they formed 2 and more shoots at tillering and had large panicles without signs of disease. The yield was 8.10 t/ha. Biochemical analysis showed that in the first variant, the flower scales of single-stem plants contained 13.8% SiO2, and in the second 19.5%. The conclusion was made that for such varieties as Leader, the high density of plant stand is unacceptable. Under conditions of increased competition, rice plants form a weak root system, accumulate little silicon, and therefore can be affected by blast disease. When studying the reaction of the plants of rice variety Leader to different levels of mineral nutrition, it has been defined that the optimal development of plants occurs at the plant density of 240-250 pcs/m2 and the level of mineral nutrition N120P100K50
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06.02.00 Veterinary and Husbandry
COMPARATIVE CHARACTERISTICS OF DI-AGNOSTIC LABORATORY TESTS FOR THE VIRUS PERITONITIS OF CATS
06.02.00 Veterinary and Husbandry
DescriptionThis article is devoted to the study of various laboratory tests for the diagnosis of viral peritonitis of cats, conducted in 2014-2018. The study was subjected to 278 cats of both sexes, different breeds and mongrel, of different ages, which were taken to the veterinary clinic «Vita» and the veterinary clinic «Noah's Ark» in Krasnodar. Analysis of the results showed that in the diagnosis and differential diagnosis of viral peritonitis of cats, the most resultant is a combination of studies of Globulin in serum and a test for antibodies to coronavirus, or a solid enzyme immunoassay (tIFA) on IgG to coronavirus. In addition to clinical symptoms, routine laboratory studies, instrumental studies for the diagnosis of viral cat peritonitis, it is necessary to use additional research methods, although many of them have a number of shortcomings in sensitivity and specificity
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06.02.00 Veterinary and Husbandry
DescriptionPasteurella multocida is an important respiratory pathogen of cattle. OmpH Protein is a major protective antigen of bacteria has been well studied in avian strains. In the literature there are no data available for the study of a variety of sequence of this protein among isolates with cattle respiratory pathology. There have been described several genes associated with the virulence of the bacterium in respiratory disease of cattle, but none of the authors compared the frequency of detection of these genes with the pathogenicity for laboratory animals. The aim of our study was the development of allele-specific PCR to determine Omph-types of Pasteurella multocida and the search for new approaches to assess the pathogenicity of isolates of bacteria. Total amount of 83 isolates allocated from the lungs of calves with respiratory pathology was investigated. All isolates belonged to groups A or D (isolates 63 and 20, respectively). Among isolates of capsular serogroup A we revealed 6 types, most propagation types were A1 and A2. All isolates of capsular serogroup D were one omph- type. In 16 out of 23 farms there were identified isolates of only one omph-type, 4 - 2 types, 3 - three types. The frequency of gene hgbb - hemoglobin binding protein correlated with pathogenicity of isolates for white mice. The developed allele-specific PCR along with hgbb gene detection can be used for screening and studying the properties of antigen and circulating pathogenic isolates and selecting a candidate vaccine strains