№ 158(4), April, 2020
Public date: 30.04.2020
Archive of journal: Articles count 21, 65 kb
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06.01.05 Selection and seed production of agricultural plants (agricultural sciences)
06.01.05 Selection and seed production of agricultural plants (agricultural sciences)
DescriptionThe article discusses the method of selection using markers in the selection of parent pairs for hybridization, in the selection process and in the subsequent evaluation of parent lines, used in linear and backcross selection of tomatoes. The work was performed on tomato hybrids that combine resistance genes to tomato mosaic virus (ToMV) or tobacco mosaic virus (TMV) in their genome. The research used seeds of foreign F1 tomato hybrids recommended by the manufacturer as resistant to the tobacco mosaic virus. Most of the tomato hybrids were Dutch selection, such leading firms as De Ruiter Seeds, Enza Zaden, Rijk Zwaan. The aim of the work was to test a system of hybridization probes designed to identify a gene for resistance to the tobacco mosaic virus. The study of the genotype of tomato plants was carried out in the laboratory of molecular diagnostics of plants , equipped with modern devices for PCR analysis on the basis of NIIOZG (Krymsk). Plant material was collected in different phases of plant vegetation. pre-numbered 1.5 ml test tubes (SSI-1200-00), plastic tablets, tweezers, and distilled water were used to collect plant samples. The collected samples were stored in a refrigerator until the plant DNA was isolated. During the study, collectible tomato samples were studied, including 8 F1 hybrids and 2 lines used as controls. According to the results of genetic analysis using the marker of the TMV resistance gene, it was found that all hybrids and one control line # 175/14 have a resistance gene in their genotype, and the analysis also showed the state of the gene – homozygous or heterozygous. The results of artificial infection confirmed the results obtained by PCR analysis. At the same time, using genetic research, it is possible to determine the exact state of the gene in the plant, which cannot be determined by methods of artificial infection, since no visual differences were found between plants with a heterozygous and homozygous state of the gene. The created marker and PCR analysis method can be recommended for wide application in the selection process of tomato culture. As a result of research, it was found that modern tomato hybrids have a gene for resistance to tobacco mosaic virus (TMV) in their genotype. This was determined by genetic analysis and confirmed by a test using artificial infection
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EVALUATION OF THE SOURCE MATERIAL TO CREATE HETEROTIC HYBRIDS OF TOMATO FOR PLASTIC FOIL HOUSES
06.01.05 Selection and seed production of agricultural plants (agricultural sciences)
DescriptionThe creation of breeding material with desired properties is a principal problem, the successful solution of which is now possible with the introduction of genetic engineering technology. We have carried out an evaluation of the source material on its basis to identify samples suitable for creating heterotic hybrids with a set of economically valuable traits, including resistance to the tomato leaf curl virus (TYLCV)
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EXPERIENCE OF USING "HYPONAT BPO" DISINFECTANT FOR REFRIGERATOR CHAMBERS DISINFECTION
DescriptionThe article presents the results of production tests of previously developed refrigeration disinfection regimen “Hyponat BPO”. As a result of production tests, it was found that the disinfectant “Hyponat BPO” provides 100% disinfection of refrigerated chambers, as well as auxiliary equipment at meat processing plants. Thus, it was determined that the positive effect of surface disinfection during control according to the E. coli test culture was achieved by using a 2% solution at an exposure of 30 minutes, staphylococcus with a 3% solution and exposure of 50 minutes, and with a VLM control, a 4% solution and exposure of 60 minutes
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Description
We took 20 samples of centralized water for bacteriological studies at two farms of the collective farm “Limann” and the collective farm “50 years of the name of the October Revolution” of the Neklinovsky District of the Russian Federation and sent to the Rostov Regional Veterinary Laboratory. Thus, according to the results of laboratory studies of water, it was found that in the first farm in 7 samples out of 10 under No. 1, 2, 4, 5, 8, 9, 10, common coliform bacteria were found (the number of bacteria in 100 ml according to MUK 4.2. 1018-01), as well as thermo-tolerant coliform bacteria (the number of bacteria per 100 ml according to MUK 4.2.1018-01), in the second farm in 5 samples out of 10 under No. 3, 4, 5, 7, 8 common coliform bacteria (number of bacteria per 100 ml according to MUK 4.2.1018-01), as well as thermo-tolerant coliform bacteria (number of bacteria per 100 ml according to MUK 4.2.1018-01). According to the results of studies using the rapid test "Biocontrol" revealed that in the first farm in 2 samples out of 10 under No. 4.9 per 1 ml of bacteria 105, which indicates water pollution, and in 5 out of 10 samples No. 1, 2, 5, 8, 10 per 1 ml of bacteria 107, which indicates a strong pollution of water and is the basis for the prohibition of watering animals. In the second household, in 3 samples out of 10 under No. 4, 5, 8 per 1 ml of bacteria 105, which indicates water pollution, and in 2 out of 10 samples No. 3, 7 per 1 ml of bacteria 107, which indicates severe water pollution which is not recommended for animals. Thus, the studied water samples by the bacteriological method coincide with the “Biocontrol” rapid tests, which makes it possible to practically use it in farms
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CORRECTION OF DYSBIOTIC DISORDERS IN BEES BY USING PROBIOTIC MEDICATIONS
DescriptionThis article provides an overview of the need of the use of probiotic supplements and medications in the diets of insects, in particular bees and their families. We present a list of both Russian and foreign probiotics and their complexes, which are used in beekeeping for the treatment and prevention of various diseases, are shown. Their effectiveness is shown not only for the prevention and treatment of microbial disorders in the gastrointestinal tract of bees due to the manifestation of antagonistic properties in relation to opportunistic and pathogenic microflora, but also the possibility of their use as tools that can increase the immune status, the safety of bee families, and life expectancy. The article shows data on the positive effect of probiotics on the physiological and biochemical parameters of honeybees, activation of metabolic processes in the formation of a fat body, growth of strength and weight of larvae, development of pharyngeal glands, etc.
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Description
The problem of providing people with food remains relevant in connection with the growth of the world's population. According to statistics, by the 21st century, the production of all types of food increased by more than 20%, while at the same time, the consumption of food products per capita increased by only 6%. In Russia, the production of food products, including dairy products and beef, is not growing at an insufficient pace. In this regard, in order to stabilize and develop the livestock industry in our country, it is important to improve the material and technical base for transferring it to the intensive path of development - maximum production with the lowest labor and material costs. Based on the achievements of scientific and technological progress and a systematic approach to the production of high-quality products, an intensive direction in the livestock industry should be based, the use of highly efficient milk production technologies, based on the achievements of science and technology, and the management of cost-effective dairy cattle breeding. Of particular strategic importance is the effective improvement of the breeding qualities of cattle and the creation of conditions for the formation and effectiveness of the manifestation of genetic capabilities in animals. Currently, the problem in the livestock sector in Russia is the insufficient provision of the feed base and the introduction of rations in the technology that are adequate to the needs of highly productive animals. Statistics show that in the Russian Federation the manifestation of the genetic potential of livestock is only 60-70%. To solve this problem, fodder production should be improved; the existing set of measures should be effectively used in the development of progressive methods of forage preparation and feeding rationing, taking into account the physiology of animals. Livestock farmers of PJSC "Rodina" of the Kanevsky district of the Krasnodar region, having the status of a breeding plant for cattle breeding of the Holstein breed, reached rather high indicators for improving the genotype of dairy cattle and the effectiveness of its manifestation
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THE EFFECT OF FEED RATIONS ON THE PRODUCTIVITY OF HOLSTEIN-FRIESIAN BREED BULL CALVES
DescriptionThe article presents the results of scientific and industrial experience in identifying the effect of feed additives on the productivity and biochemical parameters of bull calves’ blood in fattening. The calves of Holstein-Friesian breed at the age of 30 days were selected as objects of research. In the diet of the bull calves of the experimental groups, we used feed additives in the main diet: probiotic feed additive “Cellobacterin+”, feed vitamin-mineral concentrate “Tetra+”, complex feed concentrate. We found that the most active weight gain is observed in the group of calves that received complex feed concentrate. In all experimental groups of bull calves, we observed a decrease in the activity of hepato-indicator enzymes and a thymol sample. Based on the data obtained, it can be concluded that the use of the complex feed concentrate containing biologically active substances and the additive “Cellobacterin+” (in comparison with the control and other experimental groups) leads to maximizing the increase in the average mass of bull calves, which is associated with the synergistic effect of biologically active substances and living microorganisms Enterococcus faecium 1 - 35. The same tendency is observed with respect to the activity of hepato-indicator enzymes - AST and ALT, which, compared with the control group, decreases by 35.6% and 28.2%, respectively
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Description
An important factor in improving the efficiency of production in the agro-industrial sector is to improve the quality of poultry products and their processing. The article presents the dynamics of the influence of stimulating drugs on the productive qualities of the black African ostrich. The reserves of increasing the production of eggs of black African ostriches and its quality due to the use of stimulating drugs were revealed. The effect of the use of the drug called "Radostin Vitasil" on the chemical composition of eggs, egg production of black African ostriches is shown. The obtained research results convincingly prove that the mass of ostrich eggs of the experimental groups significantly exceeded the control by 5.45 and 3.11%, respectively. Changes in the mass of white and yolk of the egg of ostriches of experimental groups as a result of feeding the drug "Radostin Vitasil" affected the ratio of white/yolk, which slightly decreased towards the optimal. There was a significant difference in the content of carotenoids and vitamin A in the egg yolk of the I experimental group by 10.98% and 10.05%, in the II experimental group-9.15% and 6.35%, in comparison with similar indicators in the control group, respectively. There was an excess of vitamin E level in the eggs of the experimental groups in relation to the control by 6.73 and 3.42%. The results of incubation showed that the stimulating drug "Radostin Vitasil" had a positive effect on the process of embryonic development. The calculated economic efficiency of the drug "Radostin Vitasil" confirmed the feasibility of using this drug in the production of black African ostriches eggs
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08.00.13 Mathematical and instrumental methods of Economics
08.00.13 Mathematical and instrumental methods of Economics
DescriptionIn the article, we develop the methodology of strategic planning and management of the holding on the theoretical basis of automated system-cognitive analysis (ASC-analysis). This methodology provides scientific research of any holding by creating and researching its model. The methodology includes both the synthesis, adaptation and verification of system-cognitive models of the holding, and the use of these models for strategic planning and decision support for managing the holding, as a complex, multiparametric, nonlinear system. The relevance of the research is due to the special role of holdings and other corporate integrated structures both in Russia as a whole and, in particular, in the Krasnodar region. Despite obvious system advantages, holdings face a wide range of problems related to management efficiency, ensuring their sustainable functioning, etc. The proposed methodology offers ways to solve these problems and can be successfully applied in holdings and other corporate integrated structures of various regions, volumes and areas of activity, which determines the relevance of the research topic. The level of significance and scientific novelty of the Research consists in the development of conceptual and theoretical and methodological provisions aimed at managing the development of holdings. The expected results and their significance are that the methodology developed as a result of the Research can be applied by holding companies and other corporate integrated structures and will significantly improve the quality of their management
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PRICING METHOD BASED ON THE ESTIMATION OF DEMAND FUNCTION
08.00.13 Mathematical and instrumental methods of Economics
DescriptionWhen solving some problems of economics and management at an enterprise, it becomes necessary to determine the retail price of a product or service at a known wholesale price or producer price. We offer to determine the retail price based on an analysis of a survey of potential consumers about the maximum possible price for the product or service in question. We calculate the retail price on the basis of optimizing the economic effect equal to the product of the result from the sale of one unit of goods by the demand function, which we estimate by interviewing consumers. To solve the optimization problem, we approximate the demand function using the least squares method. As examples, the linear and power models of the demand function are analyzed. Ways of further development of the proposed approach are discussed. Unresolved scientific problems are formulated. Methods for estimating the demand function in the context of a large number of repetitions of respondents and their tendency to “round numbers” require further elaboration, as a result of which the Kolmogorov criterion cannot be used to determine the accuracy of the restoration of the demand function. Various parametric and non-parametric approaches of regression analysis should be adapted to the problem of restoring the dependence of demand on price, as well as methods for solving the corresponding optimization problems