Federal state budgetary institution of science Siberian Federal scientific center of agrobiotechnologies of the Russian Academy of Sciences
Author list of organization
List of articles written by the authors of the organization
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06.02.00 Veterinary and Husbandry
Description
Pasteurella multocida is an important respiratory pathogen of cattle. OmpH Protein is a major protective antigen of bacteria has been well studied in avian strains. In the literature there are no data available for the study of a variety of sequence of this protein among isolates with cattle respiratory pathology. There have been described several genes associated with the virulence of the bacterium in respiratory disease of cattle, but none of the authors compared the frequency of detection of these genes with the pathogenicity for laboratory animals. The aim of our study was the development of allele-specific PCR to determine Omph-types of Pasteurella multocida and the search for new approaches to assess the pathogenicity of isolates of bacteria. Total amount of 83 isolates allocated from the lungs of calves with respiratory pathology was investigated. All isolates belonged to groups A or D (isolates 63 and 20, respectively). Among isolates of capsular serogroup A we revealed 6 types, most propagation types were A1 and A2. All isolates of capsular serogroup D were one omph- type. In 16 out of 23 farms there were identified isolates of only one omph-type, 4 - 2 types, 3 - three types. The frequency of gene hgbb - hemoglobin binding protein correlated with pathogenicity of isolates for white mice. The developed allele-specific PCR along with hgbb gene detection can be used for screening and studying the properties of antigen and circulating pathogenic isolates and selecting a candidate vaccine strains
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06.02.00 Veterinary and Husbandry
Description
In the diagnosis of subclinical, serous and catarral mastitis of cows a wide range of microorganisms has been isolated, including the genera Streptococcus (Str. disgalactiae, Str. agalactiae, Str. pyogenes), Staphylococcus (St. aureus, St. epidermidis) и E. coli. When treating cows with mastitis with argovit, the average duration of treatment for animals was2.9 +0.1, 3.3 + 0.3, 4.1 +0,2 days, which is 1.6, 1.9, 1.8 times less compared with the drug spectromast in the control group. The effect of argovit containing silver nanoparticles on antibiotic sensitivity in selected microflora in the treatment of subclinical, serous and catarrhal mastitis of cows was studied. Studies conducted to determine the antibiotic sensitivity of isolated microorganisms after the treatment of mastitis of cows showed the dependence of the results, both on the drug used and on the clinical form of the disease. It has been established that the use of spectromast causes a pronounced decrease in19 (76%) preparations with subclinical, 24 (96%) serous and 23 (92%) catarrhal mastitis. It was also noted that with the development of the pathological process, an increase in the appearance of previously absent resistance to antibacterial drugs was observed with subclinical6 (24%), serous 7 (28%), catarrhal 8 (32%) mastitis of the cows. While using argovit, an increase in antibiotic sensitivity to 21 drugs (84%) in subclinical, 24(96%) in serous and 23 (92%) catarrhal mastitis was revealed. In the treatment of subclinical mastitis in the experimental group, a slight decrease was observed for 4 (16%) drugs
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