State Scientific Organization North Caucasian Regional Research Institute of Horticulture and Viticulture of the Russian Academy of Agricultural Sciences
Author list of organization
List of articles written by the authors of the organization
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STABILIZATION OF MRNA CEREALS IN VITRO UNDER THE SILICON INFLUENCE
DescriptionThe differential stability of mRNA is an important mechanism for posttranscriptional regulation of gene expression in eukaryotes. Messenger RNA stability is controlled by specific genes and growth conditions. The review examines the theoretical possibility of mRNA stabilization in vivo as a consequence of replacing carbon atoms (C) or phosphorus (P), composed of nucleic acid on the silicon (Si) atom. During isolation of poly-(A)+mRNA from plant tissues by the two-cycle affinity chromatography on poly-(U)-Sepharose, regular changes in poly- (A)++mRNA yield were observed. The changes varied both with the plant genotype and growth conditions. Celite treatment of heated and unheated total RNA preparations from developing corn kernels and from green and etiolated wheat seedlings. Messenger RNA that differed in the length of poly- (A)-sequences was used for hybridization. It is evident that a reduction of poly-(A)-length causes alterations in spatial structure of mRNA, and associated proteins and cations Mg++ become accessible to celite absorption. Heating promotes melting of secondary structure, already initiated, and increases the efficiency of mRNA stabilization by celite. Interpreting the facts interact celite with mRNA in vitro and stabilization of mRNA in vivo by cycloheximide with a modern point of view can be considered with the position research of the phenomenon of RNA interference
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CLONALE MICROPROPOGATION AND SANITATION OF PRUNUS DOMESTICA FROM THE PLUM POX POTYVIRUS (PPV)
DescriptionIn the article we present the analysis of the results of studies of russian, foreign scientists, as well as our own data, obtained in the clonal micro-multiplication and sanitation of the plum domestic from Sharkey's virus (PPV). The urgency of work is determined by the fact that the plum is the second on the significance culture in horticulture of the south of Russia. One of the most economically significant objects of virus etiology on the discharge of relative is considered to be Sharkey's virus of plum (Plum pox potyvirus). In the Krasnodar region and in the Russian Federation for the first time, Sharkey's virus of plum was discovered in the end of the 1980th, after which it widely extended into other regions of the country. In the complex of the methods of sanitation from Sharkey's virus of plum we successfully use thermo- and chemotherapy in combination with the method of apical meristem. The mechanism of the formation of virus-free meristem consists of the delay of the process of the replication of virus particles from the rapid, anticipating increase in the rudimentary cloths and organs, especially, if plant undergoes by thermo- or chemotherapy. As virucide there are used the preparations neo -DHt (85 mG/l), and also salycilic acid in the concentration of 3kh10-4 M with the simultaneous magnetic-pulse working of mericlons. As the most suitable nutrient medium for the micro-multiplication of plum we considered Murasige- Skoog medium, on basis of which we have prepared different modifications. As the growth factors, which increase the effectiveness of multiplication, we used 6-BAP in the concentration 0,5-1 mG/l (in the stage of introduction into the culture and the stage of animated cartoon). For the improvement in the quality of the micro-shoots of plum, additionally to the growth factors, one should apply succinic acid, succinates of potassium and sodium in the concentration 4 mG/l. Obtained in the course of clonal micro-multiplication and adapted mericlons will be tested to the virus carrying ability and will be approved according to the quality signs. The ovaries of initial plants are embedded by healthy quality seedlings
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Description
Sunflower is one of the most important oilseed crops, which are of great economic importance in Russia and in the world. It is very adaptive crop in terms of climatic conditions range, for that reason it is cultivated on a vast territory of the Russian Federation in various weather conditions. Sunflower oil has high nutritional and taste qualities, it is used numerously in food and is applied in various fields of food industry. The biologically active linoleic acid, phosphatides and fat-soluble vitamins A, D, E and K, which are of great nutritional value to humans, are contained in the oil. According to its calorific capacity, sunflower oil is on the first place among vegetable oils. Due to biological characteristics of sunflower, the Krasnodar region is the most favorable region of the Russian Federation for obtaining high and stable yields of this crop. However, here the drought is observed during the summer period and it affects adversely the productivity and quality of sunflower seeds. The increasing of resistance to unfavorable weather conditions is possible only based on detailed study of physiological features of productivity formation and seeds quality that is highly important task in view of the current geopolitical situation in Russia. One way of solving this issue is the appliance of the growth regulators, possessing anti-stress activity that improve the quality of sowing seeds and increase the productivity and plant resistance to stressful environmental factors. These drugs include growth regulator called Furolan, which was created in KubGTU and is certified for use in Russia. It is not toxic and is used in nano-dozes, there is no its residual quantities in the products and environment. Furolan has a positive effect on physiological and biochemical processes, improves the productivity of plants, their resistance to unfavorable growing conditions by increasing the resistance to dehydration as well as to the risk of fungal diseases
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Description
In the course of the work, 33 ISSR markers were evaluated for efficacy in the detection of genetic changes in regenerants of Galanthus woronowii Losinsk.. Ten markers were found suitable for genotyping according to the species under study. Five samples from the selected ten were analyzed for a sample of 20 plants of regenerants and a mother plant. The obtained data testify to genetic stability of plant material in the process of microclonal propagation
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Description
Microsatellite DNA markers are currently used effectively in the study of the genetic diversity of the gene pool of fruit crops and DNA certification of varieties. For plum now there is rather limited list of works on the development of this type of DNA markers. Most often, the search for new SSR-markers for this species is carried out by checking of crossreproducibility of SSR-markers developed in other species of the genus Prunus. In this study, for the 18 SSR-markers previously developed on a peach, there was performed testing and evaluation of the prospects for the use of the genotyping of plum cultivars. Testing was made on the 4 varieties of genetically distant, belonging to the 4 different subspecies of Prunus domestica L., showed the effectiveness of their use. During the study, all tested DNA-markers were grouped together in multiplex sets comprising 3-4 markers. This allows simultaneous genotyping of 3-4 loci in a single PCR reaction. These multiplex kits are available for use in the study of genetic polymorphism of species Prunus domestica L
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COMPARATIVE ASSESSMENT OF THE CLONE OF STOCK М9ЕМLA AVERAGEGROWING APPLE-TREE
DescriptionIn FSBRI «North-Caucasus zonal scientific-research institute of horticulture and viticulture» (Krasnodar) there was carried out the comparative economicbiological study of the average-height apple-tree stock М9ЕМLA in comparison with initial weakgrowing stock М9 in manifold, nursery and garden. Manifold bushes of the clone M9ЕMLA is quite worse in force than the stock М9 and were less branchy. Output of standard per hectare of manifold at the clone M9ЕMLA was on 35,5 thousand units more, and а standard condition of cuttings - on 13,2 % higher, than at the stock М9. Cuttings of the clone were less spiked and better rooted. In the first field of nursery, the plants of the clone had a vertical growth of the central conductor, slightly weaved and were more suitable to inoculation. The output of standard plants, in the variant with the use of clone M9ЕMLA by the variety Champion, in average for 2006-2010 was on 3112 units/hа, and on the variety Prikubanskoye – correspondently on 2470 units/hа higher than grafted on the stock М9. The cost price of 1000 seedlings on the clone M9ЕMLA was on 1962 rubles less than on the stock М9, and the standard of profitability of seedling growing, on the contrary was on 14-19 % higher. In the garden the trees on the clone M9ЕMLA were worse in growth of trees grafted on М9: on volume of the crone on 0,4-0,6 m3 , in diameter of the stem on 9-12 mm, in dependence on the variety, independently on the fact that some trees on clone were more weaker than grafted on the stock М9, their productivity for 2011-2015 was higher as from one tree as from the unit of area. The intensity of fruiting expressed in yield in kilos from a tree on the unit of volume of the top of a tree on the clone M9ЕMLA was on 33-39% higher than at the trees on the stock М9
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RESOURCE-SAVING AS A FACTOR OF INCREASING THE EFFICIENCY OF INDUSTRIAL FRUIT AND VINE GROWING
DescriptionThe interrelation of efficiency of reproduction with rational structure of the organization and resource capacity of productive and technological processes is established. The need in imbalances of elimination for the organization of reproduction processes is caused. The main directions of efficiency increase of industrial fruit growing and vine growing are developed: the rational structural organization of productive and technological processes, the application of resource-saving technologies of crops cultivation, the decrease in level of chemical and technogenic impacts on agric cenosises. The criterions characterizing effective results and optimal resource capacity of technologies of long-term cultures cultivation are defined. The characteristic of decrease ways of resource capacity on the basis of new constructional decisions in the forming agric cenosises is given: in the industrial fruit growing is application of the unsupported cultivation of fruit plants providing the differentiated application of a wide range of rootstocks of the SK series which without influence on heredity of the grafted variety, reduce the force of plant growth, provide a fast fruitage and high productivity, resistance to biotic and abiotic stressor; in the industrial vine growing is the application of the formations of new type reducing the costs for the making of support-trellis construction, on the carrying out of agric and technological time-limits that is reflected in product cost and profitability of production of branch production. The main directions of resource-saving are systemically presented, the assessment of their efficiency in the industrial vine growing by the principle of a golden rule of economy" is carried out
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06.00.00 Agricultural sciences
DescriptionThe features of seeding and vegetative reproduction of Hibiscus Syrian (Hibiscus syriacus L.) in the South regions of Russia are accounted in the article. It is shown that the not sorted Hibiscuses reproduce better by seeds. Sowing is made before winter (November). The young growth appears on April-May of next year. The seedlings of first-year are wintering badly in the open soil; therefore, it is better to keep them in the basements in sand. When one-year seedlings are planting next spring we receive the standard seedling in the autumn this year. Planting material of high quality of Hibiscus Syrian was grown by means of inoculation and by the grafting method. It is recommended to execute an inoculation in the first tenday period of September. It is possible to execute the reproduction by means of inoculation in case of deficit of shoots of high quality plants and for the rapid fixing of sort. It is preferable to reproduce a Hibiscus Syrian by wood shoots in the frames or by green shoots in the unheated greenhouses in an autumn-winter period. The best results in the carried out research were got at planting shoots for further growth in the containers at the greenhouses with creating of shadow in a summer period. The high speed of shoots growth in the containers of cv. Duc de Brabant, cv. Woodbrige and cv. Russian Violet varieties are observed. The vegetative mass of shoots of cv. Speciosus, cv.Carneus Plenus and cv. Red Heart varieties increases more slowly. A conclusion follows, that the studied crop must take the deserving place in the assortment of decorative bushes of plant nursery farms
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06.00.00 Agricultural sciences
DescriptionThe article presents results of the assessment of the efficiency of non-hormonal preparations which were not earlier applied in culture in vitro with high physiological activity (the preparations received by production of furfural, and also derivatives and compositions of organic acids) during regenerations of microshoots of plum, comparison of their influence with influence of growth regulators which are traditionally used in clonal micropropagation. These experimental preparations were received when processing waste of agricultural production. In this work we used: technology of clonal micropropagation of plants of in vitro, statistical data processing by method of the dispersive analysis. The objects of researches were microshoots of plum of a Stanley variety. We have established that on mediums with the preparations "Universal", sodium succinate, potassium succinate, amber acid, L-1 the large, intensively colored plum microshoots develop surpassing control (medium with BAP of 1 mg/l, IBA of 0,1 mg/l, gibberellic acid of 0,5 mg/l) in morphometric parameters. Thus, the preparations "Universal", sodium succinate, potassium succinate, amber acid, L-1 in concentration of 4,0 mg/l proved as the growth factors which are favorably influencing on plantlets’ regeneration and a qualitative condition of microshoots of plum
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Description
Sunflower is one of the most important oilseed crops, which are of great economic importance in Russia and in the world. It is very adaptive crop in terms of climatic conditions range, for that reason it is cultivated on a vast territory of the Russian Federation in various weather conditions. Sunflower oil has high nutritional and taste qualities, it is used numerously in food and is applied in various fields of food industry. The biologically active linoleic acid, phosphatides and fat-soluble vitamins A, D, E and K, which are of great nutritional value to humans, are contained in the oil. According to its calorific capacity, sunflower oil is on the first place among vegetable oils. Due to biological characteristics of sunflower, the Krasnodar region is the most favorable region of the Russian Federation for obtaining high and stable yields of this crop. However, here the drought is observed during the summer period and it affects adversely the productivity and quality of sunflower seeds. The increasing of resistance to unfavorable weather conditions is possible only based on detailed study of physiological features of productivity formation and seeds quality that is highly important task in view of the current geopolitical situation in Russia. One way of solving this issue is the appliance of the growth regulators, possessing anti-stress activity that improve the quality of sowing seeds and increase the productivity and plant resistance to stressful environmental factors. These drugs include growth regulator called Furolan, which was created in KubGTU and is certified for use in Russia. It is not toxic and is used in nano-dozes, there is no its residual quantities in the products and environment. Furolan has a positive effect on physiological and biochemical processes, improves the productivity of plants, their resistance to unfavorable growing conditions by increasing the resistance to dehydration as well as to the risk of fungal diseases