State Scientific Organization North Caucasian Regional Research Institute of Horticulture and Viticulture of the Russian Academy of Agricultural Sciences
Author list of organization
List of articles written by the authors of the organization
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Description
The article defines types of functional stability of reproduction processes. We have proved the need of identification of interrelations between factorial and productive signs, their nature and extent of influence by types of stability (eco-economic, technological-economic, financial and economic) and efficiency (eco-economic, technological, economic). The generalized characteristic of the models and methods used for detection of dependences of the functional interrelations and interferences determining functional stability and efficiency by stages of reproduction process is given. Eco-economic stability of system is provided when the balanced coherence of interrelations and interferences in the optimum range is observed: "ability of self-reproduction – anthropogenous loading", or "an initial (standard) state – resource expenses – compensations", on each element of reproduction processes. Technological and economic stability is formed by an optimality of interrelations "process efficiency – productional effectiveness"; "productional effectiveness – economic efficiency" which allow to determine parameters of rational sufficiency of realization of productional potential of plantings and size of expenses of the economic resources providing the set reproduction level. Level of realization of reproduction potential of structural elements of an agroecosystem in comparability to the level of technogenic loading is determined by each element of an agroecosystem by the empirical and statistical method allowing to give a statistical assessment to the importance of the revealed dependences and to verify the received results. The regression models characterizing interferences of the factors influencing the level of eco-economic, technological - economic and financial and economic stability of reproduction processes are constructed
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INTRODUCTION OF NEW GRAPES VARIETIES TO THE IN VITRO CULTURE
DescriptionClonal micro-multiplication – is this the basic method of obtaining the qualitative virus-free planting material. The high quality specificity of the explants reaction of the grapes varieties to the composition of nutrient medium requires the individual selection of the medium components for the most successful multiplication in vitro. In the article we present the results of studies on the cultivation of the grapes apexes in vitro the modified medium with the reduced content of macrocells. As a result of conducted investigations we have established that for the grapes varieties of Academic Trubilin, Artemis, Gurman Kraynova, Maria Kallas, Nizina, Petit Verdo and Traminer Black the most effective introduction into the in vitro culture (acclimatization of apexes 80- 100%) occurs on modified nutrient medium to Murasige and Skoog (1962), by being differed in terms of the content macro-elements (mG/l): NH4NO3 – 1237; KNO3 – 1425; MgSO4 · 7N2O – 277,5; KN2PO4 · H2O – 277,5, of the vitamin Of v1 – 10,0 mG/l, nicotinic acid – 4 mG/l. Remaining varieties of Kishmish luchistiy, Preobrajenie, Roshfor K and Yubiley Novocherkasska in the same medium were developed slowly and in a different way
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Description
Microsatellite DNA markers are currently used effectively in the study of the genetic diversity of the gene pool of fruit crops and DNA certification of varieties. For plum now there is rather limited list of works on the development of this type of DNA markers. Most often, the search for new SSR-markers for this species is carried out by checking of crossreproducibility of SSR-markers developed in other species of the genus Prunus. In this study, for the 18 SSR-markers previously developed on a peach, there was performed testing and evaluation of the prospects for the use of the genotyping of plum cultivars. Testing was made on the 4 varieties of genetically distant, belonging to the 4 different subspecies of Prunus domestica L., showed the effectiveness of their use. During the study, all tested DNA-markers were grouped together in multiplex sets comprising 3-4 markers. This allows simultaneous genotyping of 3-4 loci in a single PCR reaction. These multiplex kits are available for use in the study of genetic polymorphism of species Prunus domestica L
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APPROBATION OF SSR-ANALYSIS FOR DNA-IDENTIFICATION OF COMMERCIAL WINE YEAST STRAINS
DescriptionThe study was performed to genotype some commercial wine yeast strains with SSR-markers. Five polymorphic SSR-markers were tested in a selection of 15 yeast strains. Tested SSR-markers showed a high level of informativeness as well as polymorphism and can be used further to analyze the genetic diversity of wine yeast
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06.00.00 Agricultural sciences
DescriptionThe results of the study of physiological and biochemical parameters of plum plants, changing when infecting plants Plum pox potyvirus (PPV) are shown. It was found that the infected leaves at the stage of full development of the content chlorophyll a reduced by 30 % in variety Stanley and by 6 % in Kabardinskaya rannaya, chlorophyll b in less than 49 % at Stanley, 37% in Kabardinskaya rannaya, carotenoids are less than 22 % in the variety Stanley and by 11% in the variety Kabardinskaya rannaya . Leaves infected with the virus pox (PPV), in comparison with not infected, the amount of protein were decreasing: in the variety Stanley by 21 %, in the variety Kabardinskaya rannaya by 28 %. In the period of active growth of the leaves change in the content of pigments and protein is more intense. The content of lignin in the tissues of leaves, infected with the virus pox (PPV), in comparison with not infected in the variety Stanley was below on 13 % and in the variety Kabardinskaya rannaya - 8 %. The total content of water of infected tissue on the stage of a full-time development is reduced in patients leaves as compared with not infected 11 % in the variety Stanley and by 1 % in the variety of Kabardinskaya rannaya. Throughout the period of development of foliage studied parameters vary depending on the variety
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06.00.00 Agricultural sciences
DescriptionThe article presents brief results of the introduction of Hibiscus syriacus L. varieties for a signs collection in the south of Russia. Since 2007, Federal State Budgetary Scientific Institution North Caucasian Regional Research Institute of Horticulture and Viticulture (Krasnodar) has been working on the introduction and researching of Hibiscus Syriacus varieties and signs collection is being formed. Introduced varieties of hibiscus are obtained in zones with a moderate temperature-humidity regime, therefore not all are easily adapted in the conditions of the south of Russia. According to the results of the research, several varieties of sources of valuable traits have been identified. Varieties with pure white petals: Totus Alba, William R. Smith, White Chiffon, White Pillar. Varieties with bright crimson color petals: Freedom, Carneus Plenus, Woodbridge, Duc de Brabant. The Blue Chiffon variety is the source of bright blue petals, Sanchonyo is a source of bright purple-crimson petals. Of great value are varieties that have an unusual combination in the color of petals, like the varieties Monstrosus and Hamabo. By the sign: a large flower (d of a flower more than 12 cm), the collection includes varieties White Chiffon, Chaina Chiffon, Pink Giant. Variety Dorothi Crane and hybrids: T-16-11, T-7-11, K-16-12 are identified as sources of round, wheel-shaped flower. With a double flower type, the varieties are represented by varieties: Blue Chiffon, White Chiffon, China Chiffon, Leopoldii, Speciosus, Lucci, Sanchonyo, Freedom, Carneus Plenus. Varieties: Woodbrige, Russion Violet, Ledy Stenly, White Chiffon, China Chiffon, Purple Pillar are sources of abundant long (more than 3 months) flowering. With a high degree of self-purification after flowering, varieties are distinguished: White Chiffon, Sanchonyo, Purple Pillar, White Pillar, Freedom. Varieties: China Chiffon, Freedom, Lucii, Leopoldii, RussionViolet, hybrid forms: T-16-11, T-18-11, T-21- 12 are characterized by increased adaptability to abiotic factors in south of Russia. Dedicated varieties and forms - sources of valuable economic and biological characteristics are of interest for involving in selection and allow to model varieties with given properties
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ДНК-ПАСПОРТИЗАЦИЯ СОВРЕМЕННЫХ РОССИЙСКИХ СОРТОВ РИСА С ПРИМЕНЕНИЕМ SSR-МАРКЕРОВ
DescriptionIn the presented study, we have performed genotyping of modern Russian rice cultivars using microsatellite DNA-markers. The markers showed different level of allelic polymorphism: from 2 to 8 alleles per locus. For all studied cultivars,unique DNA-fingerprints were obtained
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Description
Professional apple gardening is bound to particular risks, of which is essential losses of a harvest because of diseases. An apple scab, the caused Venturia inaequalis (Cooke) G. Winter, brings the greatest loss. The main approach in monitoring of a scab of an apple-tree is creation of grades, steady against pathogen. In the present work we have carried out phytopathology testing of generations of the seedlings received from the free pollination of six forms of a crab of Malus orientalis from a collection MOS VIR (Maykop) characterized by a relative resistance to the apple scab in 2-3 classes of damage by long-term data. For infection we used inoculum, consisting both of natural population of a scab, and of strains of pathogen of various cultivars and geographical origin. When carrying out padding infection increase in force of an infectious background that can be bound to selection of the plants of biotypes of a fungus that are most adapted for genotypes was noted. It is recommended for precise elimination of unstable plants at selection at early stages of an ontogenesis to carry out more than one serial infection during the season. By results of the phytopathological testing, we highlighted three Malus orientalis forms from the six studied No. 17982, 17985 and 3080 the most perspective stability genes for an introgressiya in a cultural gene pool of an apple tree
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SYSTEM-WIDE INDICATORS AND ASSESSMENT OF STABILITY OF REPRODUCTION PROCESSES IN BRANCH PRODUCTION
DescriptionMethodical approaches to assessment of stability of reproduction processes which cornerstone formation of system of the balanced indicators which is based on coordination of functional and system-wide stability is are developed. The system of estimated indicators of stability is offered. The analytical model of assessment of stability providing the choice of criteria of efficiency and formation of system of estimated indicators is developed, as well as the analysis of dynamics of the external influencing factors, productive and economic indicators, financial and economic indicators; identification of the most characteristic and significant functional interrelations and dimension of interference of factors; assessment of reproduction opportunities of subjects of branch business. Calculation of an integrated indicator of financial and economic stability is performed. The complex assessment of stability providing combination of private indicators of eco-economic, technological and economic, financial and economic stability in an integrated indicator by means of methods of multidimensional correlation is given. The analysis of the factors influencing the level of stability and efficiency allowing estimating reproduction opportunities of the economic entities making grapes and wine-making production to define interrelations and interferences between resultant indicators and factorial signs and also to designate the arising disproportions is carried out. The generalizing characteristic of the macroeconomic, market and production factors influencing stability of reproduction processes in industrial wine growing is given. Based on the carried-out analysis of the factors causing stability and efficiency of reproduction processes in industrial production of grapes, the main reasons reducing stability of branch production are established
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STABILIZATION OF MRNA CEREALS IN VITRO UNDER THE SILICON INFLUENCE
DescriptionThe differential stability of mRNA is an important mechanism for posttranscriptional regulation of gene expression in eukaryotes. Messenger RNA stability is controlled by specific genes and growth conditions. The review examines the theoretical possibility of mRNA stabilization in vivo as a consequence of replacing carbon atoms (C) or phosphorus (P), composed of nucleic acid on the silicon (Si) atom. During isolation of poly-(A)+mRNA from plant tissues by the two-cycle affinity chromatography on poly-(U)-Sepharose, regular changes in poly- (A)++mRNA yield were observed. The changes varied both with the plant genotype and growth conditions. Celite treatment of heated and unheated total RNA preparations from developing corn kernels and from green and etiolated wheat seedlings. Messenger RNA that differed in the length of poly- (A)-sequences was used for hybridization. It is evident that a reduction of poly-(A)-length causes alterations in spatial structure of mRNA, and associated proteins and cations Mg++ become accessible to celite absorption. Heating promotes melting of secondary structure, already initiated, and increases the efficiency of mRNA stabilization by celite. Interpreting the facts interact celite with mRNA in vitro and stabilization of mRNA in vivo by cycloheximide with a modern point of view can be considered with the position research of the phenomenon of RNA interference