State Budget Educational Institution of Higher Education of the Republic of Crimea Crimean Engineering and Pedagogical University
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THE METHODS OF REPRODACTION OF LAVANDULA ANGUSTIFOLIA MILL.
Description
The article presents results of the study of lavender reproduction (Lavandula angustifolia Mill) with methods of generative reproduction (seeds), vegetative reproduction (cutting, layening, divide of buch), clonal microreproduction using the culture in vitro. The method of cloned microreproduction based on the culture of the isolated meristems which provides genetic identity of regenerated plants to initial forms and high coefficients of reproduction, improvement of landing material from fungal and bacterial infection, and also release from viruses at a combination with methods of thermotherapy and a chemotherapy is the most effective for the solution of objectives of seed farming of a lavender. As a material for carrying out researches we had plants of lavender (Lavandula angustifolia Mill), Sineva sorts, Stepnaya and perspective selection samples 337-9 and 310-17. Apical meristems 0,2-1,0 mm high isolated from top and axilary buds of a stalk of annual plants have been used as explant. Murasige and Skuga (MS) used as a basic nutrient medium for cultivation of the isolated meristems. Explants have been cultivated in the cultural room with 25-26 0C - temperature, lighting – 2-3 lx, relative humidity – 60-70%. It has been established that both April and October are the best month for isolation of meristems correspond calendar to phases of spring and autumn growth at the lavender donor plants and that optimum is an agar nutrient medium of MS, added with kinetin (1,0 mg/l) and GC (1,0 mg/l); frequency of regeneration of all studied genotypes was 90,0-100,0 %. Feature of morphogenesis of lavender meristems in vitro culture was already at the first stage of clonal microreproduction as there was a multiple shoot formation. Studying of features of development of lavender microplants during ten passages also was carried out, as the level of stability of regeneration processes throughout several cycles of a miсrograftage is one of important factors on which efficiency of microreproduction depends. We have also shown, that the efficiency of reproduction remains at the stable level of the sort named Sineva and sample #337-9 to the 8th passage (1:7,77-12,45 and 1:7,60-11,85 respectively), at the variety called Stepnaya - to the 7th passage (1:6,10-11,81), at the sample #310-17 - to the 6th passage (1:6,17-8,37)
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