FGBUN Mountain botanical garden of the Dagestan scientific centre of RAS
Author list of organization
List of articles written by the authors of the organization
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THE VARIABILITY OF TAXUS BACCATA CYPRESS CONES AT POPULATION OF DAGESTAN
Description
In the populations of Taxus baccata of Foothills and Inside Mountain Dagestan, we have studied intra- and interpopulation variability of morphological characters of yew berry. The article has a contribution of interpopulation differences in the feature of "diameter of funnel" with the highest rates in Buynaksk population and dependency of the form of yew berry from this feature. According to the results of discriminant analysis, we have revealed a distinction in populations on this basis, although the classification matrix has not given 100% of the forecast for any of them. It is found, that the seeds of Khunzah population have relatively large dimensions and yew berry has more flattened shape compared to piedmont populations, while the differences in populations based on seeds are more pronounced than based on yew berry at low volatility of the characteristics of the first indicators. It is shown, that the characteristics of seeds and yew berry of Khunzakh population indicate the presence of certain genetic differences between populations inside mountain and foothills, and that the higher dissemination activity of first population is aimed at self-preservation, due to the less favorable conditions for the species. It is expected that the low variability of the average values for yew berries of cypress characteristics indicates the stability of the soil and climatic conditions of its habitats
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DEVELOPMENT OF MULTIPLEX SETS OF SSR MARKERS FOR GENOTYPING APRICOT VARIETIES (PRUNUS ARMENIACA L.)
Description
Genetic studies of apricot are the actual direction in the genetics of fruit crops. In this regard, the improvement of the collection of SSR markers for the genotyping of this culture is an objectively significant task. In a study for the 16 SSR-markers previously developed on almonds (PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1, PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1) and Siberian apricot (A3-72, A1-63, H2-22, A3- 7-1, H2-5, A1-7, A3-9, H2-45), approbation and evaluation of the prospects of using for genotyping Prunus armeniaca L. were performed. Approbation, performed on 3 varieties of different origin, revealed markers and their combinations optimal for their use. During the study, all tested DNA markers were grouped into multiplex sets, including 4 markers. This allows carrying out genotyping simultaneously on 4 loci in the formulation of one reaction. One marker (PdUnchar2) from the studied sample included in the multiplex set did not show amplification. Five markers gave a monomorphic product. The remaining 11 SSR markers allowed us to obtain polymorphic, cultivar-specific SSR fingerprints for all the studied cultivar. These multiplex sets are proposed for use in studying the genetic polymorphism of the species Prunus armeniaca L.
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