-
THE EFFICACY OF USING PHOSPHATE
SOLUBILIZING MICROORGANISMS IN
GRANULAR BIOFERTILIZERS WITH
PHOSPHATE ORE
03.00.00 Biological sciences
Description
Two granular formulations of phosphorus biofertilizers
combining rock phosphate and two highly active
phosphate solubilizing strains: Acinetobacter species
305 and Pseudomonas species 181а have been investigated.
Granules of about 3 mm in size were obtained
by contact-convective drying of a mixture of ground
ore, concentrated biomass of two different strains,
starch and glucose. Micro granules with size of 0.1-
0.5 mm were obtained by spray drying the biomass of
two different strains and application of dried cells on the particles of the ground ore. Starch was used as a
binder. In the model liquid medium it was shown that
the microorganisms have retained the ability to solubilize
mineral phosphates in granular formulations prepared.
In laboratory pot trial on marigold (Tagetes
patula) it was demonstrated that both formulations of
biofertilizer increased the dry weight of the plants to
the same level as that of chemical fertilizer - double
superphosphate, but were inferior in the concentration
of phosphorus in plants. Both formulations exceeded
the effectiveness of rock phosphate and biomass used
as biofertilizers both separately and jointly. No significant
differences were noted between the two
strains and the two granular formulations both for
plant dry weight, and the content of phosphorus
therein. Both granular formulations of biofertilizer
retained their structure and avoided aggregating over a
year of storage at 4 oC. The average persistence of
living cells in the microbeads was about 1.5%, in
granules - 32 %
pdf 366.953kb
doc 366.953kb