NPF “Sady Chechni”
Author list of organization
List of articles written by the authors of the organization
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SOME ASPECTS OF INTRODUCING IN VITRO OF NEW CLONAL ROOTSTOCKS FOR STONE FRUIT CROPS
06.00.00 Agricultural sciences
Description
The article presents the results of the study of the effect of the size of the explant clonal rootstocks for stone fruit crops of the Krymskaya OSS VIR VSL-1, VSL-2, LC-52, RVL-1, RVL-7, VVA-1, AP-1 injected in vitro and concentration vitamins and phytohormones in a nutrient medium on the efficiency of introducing in vitro of these rootstocks. The optimal size of the injected in vitro explant was determined and nutrient medium in the first stage of micropropagation was optimized. The optimal size of the dissected meristem, which provides the maximum percentage of explant initiation is 0.4 mm. The modified version of the nutrient medium based on the Murashige and Skug prescriptions with a reduced content of vitamins B1 0.1 mg / l, B6 0.4 mg / l, PP- 0.4 mg / l, and phytohormone 6-BAP - 0.3 mg / l. at the first stage of cultivation of explants of clonal rootstocks for stone fruit crops is optimal. The survival rate of explants in all tested samples of clonal rootstocks on this modified nutrient medium was in the range of 92.5-97.5%
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