North Caucasian federal scientific center of horticulture, viticulture, winemaking
Author list of organization
List of articles written by the authors of the organization
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05.20.01 Technologies and means of mechanization of agriculture (technical sciences)
DescriptionThe urgent task facing the agro-industrial complex is the improvement of storage methods for agricultural products and the development of new ones. One of the actively cultivated crops, characterized by a variety of species, valuable taste and medicinal properties – grape. It is possible to provide the population with fresh grapes of high quality through the introduction of effective storage technologies that reduce losses, preserve marketable properties and biological value. One of the effective ways to preserve the quality of grapes during storage is the use of sulfur dioxide (SO2). This gas inhibits oxidative enzymes in berries, thereby reducing the rate of development of the phytopathogen Botrýtis cinérea, which causes gray rot. At the same time, it is especially important to control the rate of release of sulfur dioxide, since at the beginning of storage it is necessary to ensure the receipt of a large amount of it, which will eliminate Botrytis spores present on the surface of the berries and stabilize the existing damage. Further, during the entire period of storage of grapes, sulfur dioxide must be supplied in minimal quantities. Such dynamics of SO2 emission can be ensured by the use of two-phase generators of sulfur dioxide. The article investigated the effects of sulfur dioxide generators on the quality of grapes during storage. The study also investigates quality indicators of grapes of several varieties zoned in the Krasnodar region under long-term storage
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MODERN TECHNOLOGIES FOR GRAPE STORING
05.20.01 Technologies and means of mechanization of agriculture (technical sciences)
DescriptionThe article gives a review of modern technologies of storage of grapes. The main tool for stabilizing the quality of grapes during transportation and storage is the use of sulfur dioxide (SO2), a gas that has properties that can inhibit non-enzymatic darkening, reduce the activity of enzymes, effectively control the development of Botrytis cinérea (a phytopathogen that causes gray rot) and acts as an antioxidant. Researchers pay particular attention to control the release rate of SO2, as it is important in the first stage storage to ensure supply of significant quantities of SO2 for a short period of time, which eliminates Botrytis spores that are present on the surface of berries and to stabilize the damages formed during transport and filling; the second step is to ensure a steady supply of the minimum quantity of the SO2, to ensure the control of microbiological spoilage. For a long storage time. To preserve the quality indicators of grapes during storage, it is possible to use a modified gas environment created in various ways, including through special types of packaging; forced saturation of the environment with gases such as oxygen, ozone or carbon dioxide. At the same time, to enhance the effect can additionally apply fungicides, antifungal ear Anti-mold®, etc. There are known technologies for complex processing of table grapes to combat post-harvest microbiological spoilage, which provide for the first stage of treatment with ozone or sulfur dioxide followed by spray treatment with Muscodor albus. An alternative to the use of SO2 is the use of essential oils and hypobaric treatment methods that ensure minimal environmental impact. Positive results during storage are provided by the use of medium-range ultraviolet radiation (UV-B) or short-wave radiation (UV-C), which positively affect the transcription of biosynthetic genes, providing an increase in the content of phenolic compounds and antioxidant activity of grapes, without affecting the content of dry soluble substances, the value of total titrated acidity and pH during storage. The analysis of modern technologies is of interest to practitioners and researchers who develop technologies for storing grapes
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05.20.01 Technologies and means of mechanization of agriculture (technical sciences)
DescriptionThe article presents the results of studying the influence of some types of packaging materials used in conjunction with Inter Fresh’s ethylene absorber (sachets) on commercial quality and losses of Alexandria zucchini, Lyubov sweet pepper and tomatoes cv. Inkas during storage. The study was carried out using StePac Xtend bags, creating a modified atmosphere. The objects of research were stored for 14 days at a temperature of 8 – 10 °С and air relative humidity of 90±3 %. One sachet for ethylene absorption was placed in Xtend bags. It was found that when stored under these conditions, the total weight loss is: for tomatoes – 0.3% when using Xtend bags and Inter Fresh ethylene absorbers, 1.8% when using traditional packaging materials; for zucchini – 0.4% when using Xtend bags and Inter Fresh ethylene absorbers, 1.6% when using traditional packaging materials. Losses associated with microbiological damage during storage of zucchini and tomatoes were not observed. During storage of sweet pepper microbiological spoilage took place, which, apparently, was the result of increased contamination with pathogenic microorganisms
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APPLE ROSETTE DISEASE IN NORTH CAUCASUS PLANTATIONS
06.01.01 General agriculture, crop production (agricultural sciences)
DescriptionThe article presents the results of a study of the apple tree rosette disease found in a collection garden and having a viral nature. The rosetting has typical signs of manifestation. Sick plants develop narrow small chlorotic leaves. Rosettes of leaves on the upper cervical and lateral shoots are formed as a result of the convergence of internodes. The spread of the rosetting in the planting reaches 10-11 %. The prevalence of viral disease varies among the 44 varieties of apple trees of different ecological and geographical origin. So, the lesion of rosetting was noted on single trees in the varieties of Gala Redi Red, Fujiko, Red Free, etc., and it was a complete defeat in the variety of Piros. A different degree of the manifestation of the disease on the trees was established: from partial manifestation on individual shoots, to aggressive damage to the entire crown of the tree. On young trees bearing fruiting (3-4 years from planting), the rosetting reduces the yield of apple trees by 36 %, ¼ part of the fruits on the trees are small in size compared to the fruits of healthy trees. The infectious nature of the rosetting is confirmed by the transfer of the disease on the most affected Piros variety using summer budding
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Description
For the effective development of modern industrial gardening, it is necessary to maximize the use of the biological potential of cultivated varieties of fruit crops. In order to identify the most valuable for use in breeding and production of varieties and elite forms of apple regional and foreign selection studied the features of growth and fruiting of apple genotypes. The objects of study are 14 varieties and 2 elite forms of apple. In the work, programs and methods of selection and sorting studies that are generally accepted and developed with the participation of employees of the FSBSI NCFSCHVW were used. According to the results of many years of research, low-growing apple varieties that are promising for breeding and production have been identified: the Golden Crown, Carmen, Favorite Dutovoy, Red Chif, Sunrise, Novella, Talisman, and the elite form 12/2-20-35. Low-growing varieties with a convenient vertical crown are distinguished: Carmen, Favorite Dutovoy, Red Chief. Selected varieties of regional breeding: immune to scab Carmen and highly resistant to scab Favorite Dutovoy, possessing a complex of significant features: fruitful, low growth, with a vertical form of crown as the most valuable for use in breeding and production
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Description
The creation of high-quality grape varieties of domestic breeding is relevant in order to successfully import substitution. The purpose of these studies was to isolate and study the hybrid forms of the technical direction for the creation of promising high-quality grape varieties of domestic breeding, tolerant to phylloxera and with high quality wine-making products. The objects of research were the breeding forms of grapes of the technical direction, growing in on the hybrid section AZOSViW - a branch of the FSBSI NCFSCHVW. The studies were performed using modern and generally accepted programs and techniques. The selection of elite forms according to a complex of attributes based on many years of field and laboratory research is one of the main stages of the selection process. As a result of studying the potential of hybrid forms of grapes, the selection of AZOSViW of the technical direction highlighted the hybrid forms of grapes to the elite: III -59-21, III - 62-9, with good quality of final products and tolerance to phylloxera. The article presents a brief description of selected elite forms of grapes. The selected hybrid forms belong to the early-medium term of ripening, accumulate a high concentration of sugars and have an average cluster weight greater than that of the control variety called Krasnostop Anapa
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DEVELOPMENT OF MULTIPLEX SETS OF SSR MARKERS FOR GENOTYPING APRICOT VARIETIES (PRUNUS ARMENIACA L.)
DescriptionGenetic studies of apricot are the actual direction in the genetics of fruit crops. In this regard, the improvement of the collection of SSR markers for the genotyping of this culture is an objectively significant task. In a study for the 16 SSR-markers previously developed on almonds (PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1, PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1) and Siberian apricot (A3-72, A1-63, H2-22, A3- 7-1, H2-5, A1-7, A3-9, H2-45), approbation and evaluation of the prospects of using for genotyping Prunus armeniaca L. were performed. Approbation, performed on 3 varieties of different origin, revealed markers and their combinations optimal for their use. During the study, all tested DNA markers were grouped into multiplex sets, including 4 markers. This allows carrying out genotyping simultaneously on 4 loci in the formulation of one reaction. One marker (PdUnchar2) from the studied sample included in the multiplex set did not show amplification. Five markers gave a monomorphic product. The remaining 11 SSR markers allowed us to obtain polymorphic, cultivar-specific SSR fingerprints for all the studied cultivar. These multiplex sets are proposed for use in studying the genetic polymorphism of the species Prunus armeniaca L.
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A MODIFIED PROTOCOL OF RNA ISOLATION FROM MATURE LEAVES OF GRAPES FOR RT-PCR
DescriptionIsolation of high-quality RNA from the tissues of perennial woody plants, including woody grape vines, is very difficult due to the high content of phenolic compounds, secondary metabolites and polysaccharides and the ribonuclease activity of destroyed tissues. Most of the existing methods require either large time or financial costs, or do not give reproducible results in the case of RNA extraction from mature grape tissues. The modified isolation protocol is based on a combination and modification of the known RNA extraction methods, taking into account the characteristics of mature grape tissues. Existing commercial kits for the isolation of RNA from plant tissues showed a low efficiency of RNA extraction from mature grape tissues, primarily associated with "varietal specificity". Reproducible results in the extraction of RNA showed CTAB-method, however, it has several significant drawbacks associated with the duration of the extraction and the complexity of the processing of an RNA preparation with a DNAase. The developed method is based on increasing the concentration of mercaptoethanol and polyvinylpyrrolidone in the extraction buffer, eliminating the stage of RNA selective precipitation via LiCl, and replacing it with deposition on a silica-based membrane (SiO2) followed by processing with DNA-ase. and increase the purity of the preparation of RNA from genomic DNA in comparison with the original method. A modified isolation protocol was developed based on a combination and modification of known RNA extraction methods, taking into account the characteristics of mature grape tissues. This solution allows to obtain reproducible quantity and quality of RNA for the subsequent synthesis of cDNA and RT-PCR
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PERSPECTIVE OF USING KRASNOSTOPS OF THE ANAPA STATION SELECTION IN THE WINE-MAKING
DescriptionCurrently, more and more popular in the world are indigenous grape varieties. Now in Russia and abroad there is a growing interest in Krasnostops - a high quality indigenous red grape varieties of technical direction. The object of research was the red technical grape variety called Krasnostop Anapa and Krasnostop AZOS, grown in Anapa ampelocalamus and wine from them. As a control, we used two classic grape varieties of varying degrees of saturation in color and extract content of the wines of Cabernet Sauvignon and Saperavi. The article presents the materials of years of research – agrobiological, technological and economic indicators of technical grades of grapes of selection of the Anapa station – Krasnostop Anapa and Krasnostop AZOS. The amount of anthocyanins in wine from Anapa Krasnostops has superior data for these components in the control options. The mass concentration of phenolic substances and their monomer forms in wine materials of grape varieties Krasnostop AZOS and Krasnostop Anapsky is higher than in the quality control of red wines-Cabernet Sauvignon, which indicates a high accumulation of phenolic substances in new grape varieties. In the years of the study of Krasnostop AZOS and Krasnostop Anapsky on organoleptic properties of wine has received good performance - and of 8.78 8.73 points. The brands of wines developed on their basis will fill up a number of high-quality table and liqueur red wines in the Russian market. These grapes have high potential and great prospects in the light of the global trend of their use in winemaking. All this allows to recommend these grades for wide introduction into production in the southern wine-growing regions of Russia
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PHENOTYPING OF HIGH-QUALITY TECHNICAL GRAPES OF AZOSVIV SELECTION IN THE AMPELOGRAPHIC COLLECTION
06.00.00 Agricultural sciences
DescriptionA variety determines the direction of use of grape products and plays a leading role in improving its quality. Preservation of genetic resources of grapes and their studying are very important for modern science. The studied material makes it possible to use the selected genetic characteristics for breeding new varieties of grapes, taking into account the requirements, and introducing them into production, as well as highlighting the most valuable varieties for transferring them to the state variety testing. Among a wide variety of grape varieties, in the ampelographic collection there are varieties of AZOSVIV selection of the technical direction: Krasnostop AZOS and Dostoiniy, for which complete phenotyping was carried out. The phenological and agrobiological information on the grape varieties studied for 2015- 2017 is collected. The presented grades of grapes of the technical direction are characterized by high quality of production. The wines from the varieties Krasnostop AZOS and Dostoiniy are competitive, characterized with high consumer properties; they enjoy the well-deserved fame and were nominees of exhibitions and holders of diplomas repeatedly. Based on the results of microsatellite profiling, the varieties showed a sort-specific general combination of alleles. At the same time, in each locus there is one identical allele, which corresponds to the fact that the varieties Krasnostop AZOS and Dostoiniy have one common parent (Phylloxer-resistant Gemete). The received formulas of a variety (DNA-passports) in practice can be effectively applied to determine the cleanliness of planting stock and plantations of grapes, specification of the parent forms of the sample and in the disputed questions of the authorship of the variety. The study was supported by Federal Agency for Scientific Organizations program for support the bioresource collections