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06.00.00 Agricultural sciences
Description
Using two microsatellite loci (HaSSR1, HaSSR3), the PCR analysis of the bollworm Krasnodar population was conducted. Molecular-genetic analysis (SSR-PCR) of different geographic codling moth populations by three microsatellite loci Ср1.63; Ср2.39 и Ср2.157 was conducted also. Based on the assessment of the frequencies of occurrence of DNA markers, the molecular-genetic structure was described and genetic diversity in the pest populations was estimated
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DNA TECHNOLOGY OF PCR ANALYSIS OF CODLING MOTH POPULATIONS BY MICROSATELLITE LOCI
Description
The DNA technology of the PCR analysis of codling moth populations by microsattelite loci is adduced in detail. It includes a list of the equipment, consumable materials and chemical agents, methods of DNA extraction, PCR analysis and electrophoresis of amplification products in polyacrilamid gel. The example of the PCR analysis of codling moth population from Krasnodar region with two microsattelite loci is adduced
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SEARCHING WAYS OF ECOLOGICAL FRUIT PLANTS PROTECTION IN ORGANIC GARDEN
Description
The Codling moth monitoring, conducted with the help of injured fruits count and mail moths capture (with pheromone traps), is given in the article. We consider that pheromone traps reliably register the beginning of the Codling moth large flight (of each generation) at high temperature and little precipitation. The pheromone traps efficiency decreases at rather low temperature and showers. In organic and ecological orchards low density populations of injurious mites are under control of their natural enemies.
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