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EXTRACTION OF DNA FROM HERBARIUM LEAVES VITIS VINIFERA L.
06.00.00 Agricultural sciences
Description
Two methods were used for DNA extraction from herbarium leaves of two subspecies: V. vinifera subsp. sativa D.C. и Vitis vinifera ssp. silvestris Gmel. DNA extraction is difficult from herbarium leaves plants because of the presence of metabolites that interfere with DNA isolation procedures and downstream applications such as DNA restriction, amplification, and cloning. The quality of DNA was checked on a spectrophotometer by measuring the level of absorption at A260/280 nm. The results showed the successful extraction from mature and dried leaves of DNA when using the СТАB-method №2. Another method hasn’t shown satisfactory results in amplification of DNA
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EXTRACTION OF DNA FROM LEAVES OF VITIS VINIFERA L.
06.00.00 Agricultural sciences
Description
Extraction of the DNA from leaves of genus Vitis is a very difficult task, as a grape has a lot of elements, which make difficult to use it in the genetic manipulation. In that article used the methods of extraction of the DNA with silica (SiO2) particles as the absorbent material for getting better quality DNA
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06.00.00 Agricultural sciences
Description
Using two microsatellite loci (HaSSR1, HaSSR3), the PCR analysis of the bollworm Krasnodar population was conducted. Molecular-genetic analysis (SSR-PCR) of different geographic codling moth populations by three microsatellite loci Ср1.63; Ср2.39 и Ср2.157 was conducted also. Based on the assessment of the frequencies of occurrence of DNA markers, the molecular-genetic structure was described and genetic diversity in the pest populations was estimated
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DNA TECHNOLOGY OF PCR ANALYSIS OF CODLING MOTH POPULATIONS BY MICROSATELLITE LOCI
Description
The DNA technology of the PCR analysis of codling moth populations by microsattelite loci is adduced in detail. It includes a list of the equipment, consumable materials and chemical agents, methods of DNA extraction, PCR analysis and electrophoresis of amplification products in polyacrilamid gel. The example of the PCR analysis of codling moth population from Krasnodar region with two microsattelite loci is adduced
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MOLECULAR MARKERS AND HOW TO USE THEM IN BREEDING AND GENETIC RESEARCHERS
06.00.00 Agricultural sciences
Description
Progress of molecular genetic developed different types of DNA-markers, which are founded on analysis of DNA-polymorphism. Using of these markers has hanged methods of genetic diversity estimation as well as plant cultivars passportisation and classification
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NOVATION OF VITICULTURE IN RUSSIA 4. DEVELOPMENT OF CLONE SELECTION IN VITICULTURE
06.00.00 Agricultural sciences
Description
The analyze of the genetic relationship or diversity between clones was made by using the microsatellites in the groups with black berries: Cabernet-Sauvignon and Merlot, and with white berries: Muscat white, Riesling and Pinot. It was possible to approve the objective evidence on the polymorphism between these forms during this analyze. These results become the rationale for the selection, breeding and making registration of these protoclones of four above-named varieties groups in the varieties-clones for state research: Cabernek, Cabernet Fanogoriisky, Cleret temrukskii, Merlok, Pinot white, Rieslinalk, Riesling Anapa, Riesling Fanogoriisky, Muscat Temruk, Shardonek and others. These varieties-clones are accepted by State commission of Russia, they will enrich the viticulture assortment of Anapa-Tamanskay zones of Kuban, therefore Russia too
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TECNOLOGY OF CLONE SELECTION WITH THE USE OF MOLECULAR MARKERS
Description
The analyze of the genetic relationship or diversity between clones was made by using the microsatellites in the groups with black berries: Cabernet-Sauvignon and Merlot, and with white berries: Riesling and Pinot. These results become the rationale for the new technology of clone selection, breeding and making registration of these clones of four above-named varieties groups in the varieties-clones for state research: Cabernek, Cabernet Fanogoriisky, Merlok, Pinot white, Rieslinalk, Riesling Anapa and Riesling Fanogoriisky. These varieties-clones are accepted by State commission of Russia, they will enrich the viticulture assortment of Anapa-Tamanskay zone of Kuban, therefore Russia
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