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EXTRACTION OF DNA FROM HERBARIUM LEAVES VITIS VINIFERA L.
06.00.00 Agricultural sciences
Description
Two methods were used for DNA extraction from herbarium leaves of two subspecies: V. vinifera subsp. sativa D.C. и Vitis vinifera ssp. silvestris Gmel. DNA extraction is difficult from herbarium leaves plants because of the presence of metabolites that interfere with DNA isolation procedures and downstream applications such as DNA restriction, amplification, and cloning. The quality of DNA was checked on a spectrophotometer by measuring the level of absorption at A260/280 nm. The results showed the successful extraction from mature and dried leaves of DNA when using the СТАB-method №2. Another method hasn’t shown satisfactory results in amplification of DNA
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EXTRACTION OF DNA FROM LEAVES OF VITIS VINIFERA L.
06.00.00 Agricultural sciences
Description
Extraction of the DNA from leaves of genus Vitis is a very difficult task, as a grape has a lot of elements, which make difficult to use it in the genetic manipulation. In that article used the methods of extraction of the DNA with silica (SiO2) particles as the absorbent material for getting better quality DNA
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06.00.00 Agricultural sciences
Description
Using two microsatellite loci (HaSSR1, HaSSR3), the PCR analysis of the bollworm Krasnodar population was conducted. Molecular-genetic analysis (SSR-PCR) of different geographic codling moth populations by three microsatellite loci Ср1.63; Ср2.39 и Ср2.157 was conducted also. Based on the assessment of the frequencies of occurrence of DNA markers, the molecular-genetic structure was described and genetic diversity in the pest populations was estimated
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THE RESEARCH OF VITIS SILVESTRIS GMEL. IN THE NOTHERN CAUCASUS
06.00.00 Agricultural sciences
Description
Vitis vinifera subsp. silvestris Gmel. is the wild subspecies of Vitis vinifera L. and an important source of genetic resources for grapes breeding. Currently, this form is very rare in the world. In this paper, conducted the first study of wild grapes which was found in the North Caucasus with the use of two microsatellite markers. The study have shown a high level of genetic diversity: intrapopulation variability was 75,2%, the interpopulation variability accounts was 24,8%. All populations are characterized by certain unique genetic structure and may be as donor of resistance genes which have a long-term evolutionary selection
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MOLECULAR MARKERS AND HOW TO USE THEM IN BREEDING AND GENETIC RESEARCHERS
06.00.00 Agricultural sciences
Description
Progress of molecular genetic developed different types of DNA-markers, which are founded on analysis of DNA-polymorphism. Using of these markers has hanged methods of genetic diversity estimation as well as plant cultivars passportisation and classification
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