INFLUENCE OF SOIL FACTORS OF TERSKO-SULAKSKAYA LOWLAND AND HUNZAHSK MOUNTAIN AREA OF DAGESTAN FOR THE INNER SOLUTION OF DOGROSE ROSA CANINA
03.00.00 Biological sciences
The characteristics of meadow-wood typical soils, located on territory of lowland of Tersko-Sulaksk and Hunzahsk mountain area of Dagestan are given. Results of research of the contents of vitamin C (an ascorbic acid), P-active connections and mineral substances of iodine, manganese, copper and zinc in the hips, growing on above-stated ground are resulted. The received data testify that the meadow-wood ground of Tersko-Sulaksk lowland promotes accumulation of iodine in a dog rose, and the similar mountain ground in a greater measure influences synthesis of vitamins and the contents in hips of mineral substances. The value researched biochemical connections for a human’s organism of the person, the is shown and the comparative estimation of their structure in a dog rose growing on meadow-wood grounds, being on various heights above sea level is given
GRAPEVINE PLANT REGENERATION FROM CELL SUSPENSIONS AND INFLORESCENCE FORMATION IN VITRO UNDER THE INFLUENCE OF DIFFERENT VITAMIN AND MINERAL ELEMENT LEVELS IN NUTRIENT MEDIA
06.00.00 Agricultural sciences
Effects of different levels of vitamins (thiamine, pyridoxine and nicotinic acid) and macro-elements (MS, NN and PG) on development of globular, heart- and torpedo-stage embryos and plantlets with green cotyledons and on shoot production from them were studied in grapevine interspecific hybrids: cvs. ‘Bianca’, ‘Podarok Magaracha’ and ‘Intervitis Magaracha’. Pro-embryogenic calli were derived from petiole explants on solid full-strength NN medium supplemented with different levels of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (BA) for each cultivar and used to initiate cell suspensions in liquid NN medium supplemented with 1 mg L-1 2,4-D and 0.2 mg L-1 BA. The following formulations of media were optimal for different stages of somatic embryogenesis and for plantlets with green cotyledons development: liquid NN medium supplemented with 0.5 mg L-1 BA for globular embryo formation; liquid HTE medium (with PG macro-elements, thiamine and pyridoxine at 5 mg L-1 each and 0.5 mg L-1 nicotinic acid): supplemented with 0.2 mg L-1 BA for heart-stage embryo development, supplemented with 0.1 mg L-1 indole-3-acetic acid (IAA) and 30 mg L-1 sodium humate for torpedo-stage embryo development and supplemented with 0.5 mg L-1 gibberellic acid (GA3) for the growth of plantlets with green cotyledons. Solid MS medium modified by the addition of nicotinic acid, pyridoxine and p-aminobenzoic acid (PAB) at 5 mg L-1 each, 0.5 mg L-1 thiamine and 0.5 mg L-1 BA was best efficient for shoot production from plantlets. Inflorescence formation in regenerated plants of cv. ‘Podarok Magaracha’ grown on solid hormone-free PG medium was induced by addition of nicotinic acid, pyridoxine and PAB at 5 mg L-1 each, 0.5 mg L-1 thiamine and 0.2 mg L-1 BA into liquid modified HTE medium for growth of plantlets with green cotyledons and into solid modified MS medium for shoot production from them.