03.00.00 Biological sciences
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Description
In the course of the work, 33 ISSR markers were evaluated for efficacy in the detection of genetic changes in regenerants of Galanthus woronowii Losinsk.. Ten markers were found suitable for genotyping according to the species under study. Five samples from the selected ten were analyzed for a sample of 20 plants of regenerants and a mother plant. The obtained data testify to genetic stability of plant material in the process of microclonal propagation
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Description
The study was aimed on approbation of 11 ISSR DNA-markers for their using in DNA fingerprinting of rare plant species of the Western Caucasus Lilium Caucasicum Miscz. Ex Grossh., Galánthus woronowii Kolak., Pancratium maritimum L. according to results DNA-markers set was selected for further using in the investigation of genetic diversity of the mentioned plant species
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Description
Microsatellite DNA markers are currently used effectively in the study of the genetic diversity of the gene pool of fruit crops and DNA certification of varieties. For plum now there is rather limited list of works on the development of this type of DNA markers. Most often, the search for new SSR-markers for this species is carried out by checking of crossreproducibility of SSR-markers developed in other species of the genus Prunus. In this study, for the 18 SSR-markers previously developed on a peach, there was performed testing and evaluation of the prospects for the use of the genotyping of plum cultivars. Testing was made on the 4 varieties of genetically distant, belonging to the 4 different subspecies of Prunus domestica L., showed the effectiveness of their use. During the study, all tested DNA-markers were grouped together in multiplex sets comprising 3-4 markers. This allows simultaneous genotyping of 3-4 loci in a single PCR reaction. These multiplex kits are available for use in the study of genetic polymorphism of species Prunus domestica L
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APPROBATION OF SSR-ANALYSIS FOR DNA-IDENTIFICATION OF COMMERCIAL WINE YEAST STRAINS
DescriptionThe study was performed to genotype some commercial wine yeast strains with SSR-markers. Five polymorphic SSR-markers were tested in a selection of 15 yeast strains. Tested SSR-markers showed a high level of informativeness as well as polymorphism and can be used further to analyze the genetic diversity of wine yeast
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Description
The study was performed to genotype some commercial wine yeast strains using the assay of Interdelta genomic sequences. Experimental parameters of PCR to identify were optimized and optimal simplified method of DNA extraction from dried preparations of yeast cultures was define. Proven method showed a high level of resolution and can be used for the analysis of genetic diversity wine yeast in combination with SSR-markers
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APPROBATION OF MICROSATELLITE DNA-MARKERS FOR IDENTIFICATION OF APPLE CLONES
DescriptionAs the result of the work, the test of microsatellite DNA markers for the identification of genomic polymorphism between apple cultivars Florina, Golden Delicious and clonal forms has been done. With the high level of intervarietal polymorphism, studied DNA markers showed no allelic differences between varieties and their clonal forms
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APPROBATION OF MULTIPLEX SSRANALYSIS FOR DNA-FINGERPRINTS OF RICE VARIETIES
DescriptionResults of testing of multiplex sets SSR-markers for genotyping of rice varieties are presented in the article. Two sets of SSR-markers were formed: 1: RM1+ RM11+ RM70+RM122; 2: RM164+RM167+RM168. The optimal combination of DNA markers in the multiplex sets and PCR conditions allowed obtaining accurate, easily interpretable results when performing fragment analysis on automated genetic analyzer ABIprism3130. Using multiplex sets, genotyping was performed for several varieties of rice: domestic breeding and one variety – IR36 from the breeding of IRRI (Manila, Philippines). For all the studied varieties specific SSR-fingerprints were obtained. RM 168 marker showed in domestic varieties a low level of polymorphism - one allele of 97 bp. However, at the same time, the variety IR-36, showed a second type of allele 107 bp. In addition, the loci of RM1, RM11, RM167 and RM164 have unique alleles in this variety. It is consistent with significant genetic differences of these varieties and the rest of the varieties in studied sample. The proposed SSR multiplexes are promising for use in DNA certification of rice varieties and assessment of genetic diversity
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PROTEINS OF AFRICAN SWINE FEVER VIRUS
DescriptionThe review presents some data on structural and non-structural, regulatory proteins and enzymes of African swine fever (ASF) virus. The variety of the virus biological characteristics is substantially caused by proteins belonging to multigenic families. It is suggested, that the protection development at ASF is provided not only by the membrane proteins, but also by the regulatory ones
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Description
In this article, we have presented a number of modeling experiments to investigate the chemical pollution of brown calcareous soil. It assesses the stability of brown calcareous soil of a nature reserve called Utrish to contamination with heavy metals in biological parameters. Pollution of brown calcareous soil with Cr, Cu, Ni, Pb and oil causes a significant reduction in biological parameters. The degree of reduction depends on the nature of the contaminant and its concentration in the soil. The study showed that the indicators used in the biological condition of the soil, could be recommended for use for diagnosis, monitoring, and regulation of chemical pollution of brown soil. According to the degree of resistance to chemical contamination, the brown soils of Russia form the following series: typical brown - brown carbonate - brown leached
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BIO-DIAGNOSTICS OF RESISTANCE OF GREY FOREST SOILS OF ADYGEA TO POLLUTION WITH Zn, Cd, Mo, Se
DescriptionThe essential part of a soil cover of the Republic of Adygea is occupied by gray forest soils. Thus they still remain a little studied, including concerning their resistance to chemical pollution. Contamination of gray forest soils of Adygea with Zn, Cd, Mo, Se causes deterioration of their biological properties. In most cases, the degree of reduction of the values of biological indicators is directly dependent on the concentration of pollutant in the soil. According to the degree of toxicity to the biological properties of the investigated elements form the following sequence: Se > Zn > = Cd > Mo. Biological parameters investigated in research (activity of catalase and dehydrogenase, cellulolytic ability, abundance of bacteria of the genus Azotobacter, radish root length) may be used for purposes of monitoring, diagnosis and regulation of chemical pollution of gray forest soils Zn, Cd, Mo, Se