03.00.00 Biological sciences
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RESOURCE POTENTIAL IN LUTEOLIN-7- GLYCOSIDE VERONICA INCANA (SCROPHULARIACEAE) IN CENTRAL YAKUTIA
DescriptionThe article reveals luteolin-7-glucoside accumulation in the overground part of Veronica incana L. It assesses resource potential of V. incana depending on the phytocenotic conditions of coenopopulations growing in Central Yakutia. Collection of the material was carried out during V. incana flowering in 7 coenopopulations and in the collection of the Yakut Botanical Garden. Analysis of flavonoids content was performed by HPLC method. The high levels of flavonoids content in the biomass of generative plants marked in coenopopulations that are in optimal conditions, and the lowest - in coenopopulations experiencing strong anthropogenic influence. In addition, one of the factors reducing the content of flavonoids in plants is low light intensity in habitat. The highest resource potential on the luteolin-7- glucoside observed in biomass V. incana, growing in fescue grassland steppe and culture
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Description
Along with traditional ways, the biotechnological methods become more significant in contribution to plant conservation in situ. The work is dedicated to elaboration and advancement of the method of isolated tissues and organs culture for conservation and reproduction of the badan, Bergenia crassifolia L., the rare species of Saxifrgaceae family. The conducted study yielded the regenerated plants of B. crassifolia from seeds. For the first time, we have performed conservation and reproduction of B. crassifolia in vitro under conditions of Yakutia
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MICROORGANISMS OF PEDOSPHERE AND PECULIARITIES OF THE SOIL COVER OF AGRICULTURAL LANDSCAPES
DescriptionThe soil by it physical and chemical properties is a poly-dispersed heterogeneous multicomponent unique environment for development of most microorganisms. The soil is the richest natural substrate according to microbial gene fund. Presence of plants and animals in soil maintains it heterogeneous as environment of soil microorganisms, which are main regulators of natural gas composition of atmosphere of Earth, including its macro- and micro-components (including the main "greenhouse" gases - methane, carbon dioxide, nitrous oxide). Recently, the elucidation of these singularities of vital activity of soil organisms has resulted in general conclusion - due to them soil cover serves as a global bio-geo-chemical membrane, through which the exchange of matter and energy between pedosphere, lithosphere, atmosphere, hydrosphere and main living inhabitants of Earth does its work
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COMPARATIVE ASSESSMENT OF THE CLONE OF STOCK М9ЕМLA AVERAGEGROWING APPLE-TREE
DescriptionIn FSBRI «North-Caucasus zonal scientific-research institute of horticulture and viticulture» (Krasnodar) there was carried out the comparative economicbiological study of the average-height apple-tree stock М9ЕМLA in comparison with initial weakgrowing stock М9 in manifold, nursery and garden. Manifold bushes of the clone M9ЕMLA is quite worse in force than the stock М9 and were less branchy. Output of standard per hectare of manifold at the clone M9ЕMLA was on 35,5 thousand units more, and а standard condition of cuttings - on 13,2 % higher, than at the stock М9. Cuttings of the clone were less spiked and better rooted. In the first field of nursery, the plants of the clone had a vertical growth of the central conductor, slightly weaved and were more suitable to inoculation. The output of standard plants, in the variant with the use of clone M9ЕMLA by the variety Champion, in average for 2006-2010 was on 3112 units/hа, and on the variety Prikubanskoye – correspondently on 2470 units/hа higher than grafted on the stock М9. The cost price of 1000 seedlings on the clone M9ЕMLA was on 1962 rubles less than on the stock М9, and the standard of profitability of seedling growing, on the contrary was on 14-19 % higher. In the garden the trees on the clone M9ЕMLA were worse in growth of trees grafted on М9: on volume of the crone on 0,4-0,6 m3 , in diameter of the stem on 9-12 mm, in dependence on the variety, independently on the fact that some trees on clone were more weaker than grafted on the stock М9, their productivity for 2011-2015 was higher as from one tree as from the unit of area. The intensity of fruiting expressed in yield in kilos from a tree on the unit of volume of the top of a tree on the clone M9ЕMLA was on 33-39% higher than at the trees on the stock М9
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INFLUENCE OF MELAPHEN ON AGROBIOLOGICAL AND TECHNOLOGICAL CHARACTERISTIC OF SAVINION WHITE GRAPE
DescriptionHereby we present the results of the research devoted to study of influence of growth modulator called melaphen on the technical brand of gapes such as Sovinion White and its agro-biological characteristics. The research was conducted in 2014-2015 using fruitful grape Sovinion White of 2004 planting as a subject in agro farm “Yuzhnaya” of Temruk in the Krasnodar Region. Melaphen is a type of melamine salt of bioximethilphosphin acid. It evokes complex influence on the energetic complex of vegetation cell in all three stages of vegetation. The chemical was applied mechanically by a tractor sprinkling. The square of tested patch is 2 hectares. Melaphen usage was 6,7 ml to 1 hectare . Norm of liquid applied was 700 liters to 1 hectare. Three times the chemical was applied during vegetation: before blossoming, grape growth phase (grapes the size of a pea) and before the grapes ripen. As a result it was estimated that three times chemical application of melaphen to Sovinion White in dose 1. 10-9 in period after blossoming of grapes and before the grapes ripen led to more saturated accumulation of sugar in juice of the grapes and also to faster ripening and harvesting, larger mass of grape bunch amount of harvesting from a bush. Besides, in the tested option growing processes are accelerated and pigmentation agents are accumulated faster in the leaves and embryo infestation in winter sleeping core is boosted at larger scale. Better harvest quality leads to better wine materials quality. Application of melaphen provides higher economical efficiency
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Description
Sunflower is one of the most important oilseed crops, which are of great economic importance in Russia and in the world. It is very adaptive crop in terms of climatic conditions range, for that reason it is cultivated on a vast territory of the Russian Federation in various weather conditions. Sunflower oil has high nutritional and taste qualities, it is used numerously in food and is applied in various fields of food industry. The biologically active linoleic acid, phosphatides and fat-soluble vitamins A, D, E and K, which are of great nutritional value to humans, are contained in the oil. According to its calorific capacity, sunflower oil is on the first place among vegetable oils. Due to biological characteristics of sunflower, the Krasnodar region is the most favorable region of the Russian Federation for obtaining high and stable yields of this crop. However, here the drought is observed during the summer period and it affects adversely the productivity and quality of sunflower seeds. The increasing of resistance to unfavorable weather conditions is possible only based on detailed study of physiological features of productivity formation and seeds quality that is highly important task in view of the current geopolitical situation in Russia. One way of solving this issue is the appliance of the growth regulators, possessing anti-stress activity that improve the quality of sowing seeds and increase the productivity and plant resistance to stressful environmental factors. These drugs include growth regulator called Furolan, which was created in KubGTU and is certified for use in Russia. It is not toxic and is used in nano-dozes, there is no its residual quantities in the products and environment. Furolan has a positive effect on physiological and biochemical processes, improves the productivity of plants, their resistance to unfavorable growing conditions by increasing the resistance to dehydration as well as to the risk of fungal diseases
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Description
Sunflower is one of the most important oilseed crops, which are of great economic importance in Russia and in the world. It is very adaptive crop in terms of climatic conditions range, for that reason it is cultivated on a vast territory of the Russian Federation in various weather conditions. Sunflower oil has high nutritional and taste qualities, it is used numerously in food and is applied in various fields of food industry. The biologically active linoleic acid, phosphatides and fat-soluble vitamins A, D, E and K, which are of great nutritional value to humans, are contained in the oil. According to its calorific capacity, sunflower oil is on the first place among vegetable oils. Due to biological characteristics of sunflower, the Krasnodar region is the most favorable region of the Russian Federation for obtaining high and stable yields of this crop. However, here the drought is observed during the summer period and it affects adversely the productivity and quality of sunflower seeds. The increasing of resistance to unfavorable weather conditions is possible only based on detailed study of physiological features of productivity formation and seeds quality that is highly important task in view of the current geopolitical situation in Russia. One way of solving this issue is the appliance of the growth regulators, possessing anti-stress activity that improve the quality of sowing seeds and increase the productivity and plant resistance to stressful environmental factors. These drugs include growth regulator called Furolan, which was created in KubGTU and is certified for use in Russia. It is not toxic and is used in nano-dozes, there is no its residual quantities in the products and environment. Furolan has a positive effect on physiological and biochemical processes, improves the productivity of plants, their resistance to unfavorable growing conditions by increasing the resistance to dehydration as well as to the risk of fungal diseases
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TO THE QUESTION OF PHENOLOGY OF СONVALLARIA MAJALIS L. IN THE CONDITIONS OF THE MIDDLE DON
DescriptionFor the conservation of biodiversity, this study of patterns of biological processes and phases in the development of Convallaria majalis L. that are repeated annually becomes actual. In the article, we have presented an analysis of five years of observations of the rhythm of the development of Convallaria majalis L. in the conditions of the middle Don. There were allocated phenological phases of lily of the valley: vegetative (beginning of sprout growth, deploying of leaves), bud formation, flowering (disclosure of the first flower, mass blossoming, the withering of separate flowers, the ending of flowering), fruitage (the beginning of fruit setting, mass of fruit setting, mass ripening of fruits), the end of the vegetation (appearance of the first changes in color of leaves, the complete drying). We have defined daily average temperature and the appropriate amount of positive temperatures for the passage of various phases of development Convallaria majalis L. By the results of two growing seasons, the optimal daily average temperature for the flowering period is 14,3 ° C (the sum of average daily temperatures 161,3-204, 0С) - until 9-15 days. At higher daily air temperatures flowering begins at lower amount of positive and effective temperatures after 40-45 days after the start of the vegetation. At lower daily air temperatures flowering is longer than at higher. In the conditions of the middle Don there were allocated some examples of Convallaria majalis L. which bloom two years in a row
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Description
The article discusses the formation of the collection that includes the most numerous genus Pelargonium (L.Herit.) from the family Geraniaceae Juss, as well as its importance, and the prospects for replenishing and use. There is also analysis of the publications for the recent years, clarifying approaches and principles in classifying the representatives of this species. The collection of Russian Research Institute of Floriculture and Subtropical Crops is described, taking into account new palynological and cytogenetic criteria in pelargoniums section division. It is represented by more than 120 species, cultivars, as well as intravarietal and interspecific hybrids and is divided into three branches (A, B, C2), three subgenus (Pelargonium. Parvulipetala, Paucisignata) and five sections (Pelargonium, Otidia, Peristera., Reniformia Ciconium). Collection samples are included to the breeding research, as sources of economic features when creating highly-ornamental hybrids and cultivars. The work also studies possible using of the most adapted species, cultivars and hybrids in urban landscaping on the Black Sea coast
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ISOLATION AND SELECTION OF THE NON TRANSDUCING E. COLI BACTERIOPHAGES FOR ANTI-COLIBACILLOSIS DRUGS
DescriptionThe isolation of E.coli phages from samples of natural and waste water obtained during expeditions in the different regions of Russian Federation was carried out. The obtained phages (286 isolates) were tested for their ability to lyse the pathogenic strains of E. coli – pathogenic agents of pig colibacteriosis in Krasnodar region. The study was conducted of their ability to phage transduction, the molecular-genetic characterization and biotechnological parameters of selected bacteriophages. For first experimental design of veterinary drugs was selected 5 coliphages having no ability of plasmids transduction. It has been shown that all the investigated phages are representatives of T4-type phages of family Myoviridae. The reported study was partially supported by RFBR, research projects No. 08-04-99111, 09-04-10132, 16-44- 230855