03.00.00 Biological sciences
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Description
Questions of microbial plant protection become more relevant. The use of microorganisms can solve the following problems: biologization of agriculture and sanitation of soil. Application of various physical factors affecting the microorganisms reduces their number. This influences the efficiency of their use. This article gives examples of the application of pressure in the biotechnological process of microbial preparations and their use in the process of spraying. The effect of pressure on nonspore bacteria - Azotobacter chroococcum, spore-forming bacteria - Bacillus megatherium and fungi - Trichoderma viride has been presented in this article. These microorganisms are used in the tank medium for protecting plants against diseases and pests developed by Ltd. "Kuban agrotehnoloogy" of Kuban State Agrarian University. Efficiency is the simultaneous application of tank medium of microorganisms with herbicide. It was found that the application of the tank medium of microorganisms for spraying is necessary to limit the pressure of 4.5 atm. Тhe use of higher spraying modes effects on reducing the number of viable bacteria. This fact has not been checked against fungi
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ДНК-ПАСПОРТИЗАЦИЯ СОВРЕМЕННЫХ РОССИЙСКИХ СОРТОВ РИСА С ПРИМЕНЕНИЕМ SSR-МАРКЕРОВ
DescriptionIn the presented study, we have performed genotyping of modern Russian rice cultivars using microsatellite DNA-markers. The markers showed different level of allelic polymorphism: from 2 to 8 alleles per locus. For all studied cultivars,unique DNA-fingerprints were obtained
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DEVELOPMENT OF OPTIMAL WAY OF OBTAINING A HYDROLYZATE OF LACTIC ACID BACTERIA
DescriptionThe work was carried out in the scientific-research laboratory of the chair of Biotechnology, Biochemistry and Biophysics of Kuban SAU, the aim of which is the search of optimal conditions of obtaining the hydrolyzates of lactic acid bacteria by thermoacid and enzymatic ways. The pure cultures of microflora of gastrointestinal tract of quails − Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius were the object of researches. Under conducting the thermal acid hydrolysis there were used the same correlations of bacterial mass and water as well as procedures of clearing of hydrolyzates. The variable conditions were рН, temperature and time of hydrolysis. When carrying out the enzymatic hydrolysis in this study there was used the enzyme from the group of muramidase lysozyme. The amount of enzymes and time of carried out hydrolysis were the variable conditions under obtaining the enzymatic hydrolyzates. In the process of researches there were studied: qualitative content of total protein, peptides with М.м. < 1500, protein and peptides with M.m. > 1500, aminoacids and as well as GMDP (glucosaminilmuramilpentapeptide). On the basis of carried out experiments there was stated that according to all components of biologically active substances the thermal acid way is more effective than enzymatic. The maximum accumulation of BAA in hydrolyzates by different ways was revealed at destruction of cells Lb. intermedius. Content of GMDP in thermal acid hydrolyzate was higher than in enzymatic on 0,028 g/100 ml (51,8 %)
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Description
The article presents the results of studies of vitality and of the correlation variability of morphological traits cenopopulations Lilium pensylvanicum Ker.- Gawl. on the natural territory of the Yakutsk Botanical Garden and in cultura. The study shows variability of their parts (variability) and the estimation coenopopulations vitality (vitality)
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Description
Researches on non-root additional fertilizing of grapes with complex water-soluble fertilizer called Nutrivant plus have been conducted in corporation "Pobeda" in Temrjuksky area of Krasnodar territory on the implanted plantings of technical grade Rhine Riesling. In the test, the following kinds of Nutrivant plus were used: oleiferous (N0P20K33), sugar beet (N0P36K24), grapes (N0P40K25), universal (N19P19K19), grain (N6P23K35). Each mark of Nutrivant plus contained a certain set of microelements. The test consisted of six variants:1 variant - without additional fertilizing(control); 2 variant: grapes - before flowering, grapes - a growth phase of berries (a berry about a pea), oleiferous - the beginning of maturing of berries; 3 variant: universal - before flowering, universal - a growth phase of berries (a berry about a pea), oleiferous- the beginning of maturing of berries; 4 variant: grain - before flowering, grain - a growth phase of berries (a berry about a pea), oleiferous- the beginning of maturing of berries; 5 variant: Sugar beet - before flowering, a sugar beet - a growth phase of berries (a berry about a pea), a sugar beet - the beginning of maturing of berries; 6 variant: universal - before flowering, grain - a growth phase of berries (a berry about a pea), grapes - the beginning of maturing of berries. Norm of the expense of fertilizer is 3 kg/hectares. The expense of a working liquid - 700 l/hectares. The result was, that in the third, fourth and fifth variant of the test the increase in a crop from a bush happened accordingly on 7,5, 13,8 and 17,8 %. The maximum increase of a crop in the fifth variant was accompanied by increase in mass concentration of sugars in juice of berries on 0,6 g/100 см3и decrease titratable acidities for 0,6/dm3 As a result triple not root additional fertilizing of grapes plants grade Riesling with Nutrivant plus (universal - before flowering, grain - in a growth phase of berries and grape - in the beginning of maturing of berries) promoted more optimization of a water mode of leaves, increase in weight of a cluster, a crop from a bush and productivity, mass concentration of sugars and phenolic substances in juice of berries
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Description
The results of scientific investigations in specifying environmental and faunal composition of soil invertebrates at agricultural landscapes of steppe zone of the Krasnodar region are presented this article. Observed territory is represented with four main classes of animals: Insecta (insects), Crustacea (cancroid), Myriapoda (myriapod), Olygochaeta (worms with small bristles). Those animals were the subject of further descriptions of territory. Some peculiarities and regularities of quantity and taxonomic compound of mesofauna, depending on technological methods that are being used for cultivation of agricultural crops in experimental farming JSC “Zavety Il’icha” of the Leningrad district, are considered. The observed area is mostly populated with soil inhabitants (68,7%) that include such families as Geophilomorpha (geofila), Lithobiomorpha (drupes), Julidae (millipede), Enchytraeidae, Lumbricidae (earth worms). It was found that the use of complex compost (as an alternate method of cultivating the winter wheat and the sugar beet) has a positive impact on a biological activity of ordinary chernozem. The introduction of complex compost optimized the ecological state of agrolandscape system, which is associated with activation of biophilic elements at upper soil layer. An upgrade of soil structure, water-holding capacity and agrochemical properties creates enabling environment for the vital activity of invertebrates and for their trophic cooperation with the habitats
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USE OF EPIN-EXTRA, R PREPARATION ON SEED PLANTS OF ONION
DescriptionAn important element in the technology of growing seed plants of onion is the use of physiologically active substances to protect plants from frost in early spring growing season. Damage to plants during this period affects seed production. In the experiment we used preparation called Epin-Extra R, with which the leaves of plants were treated a day before expected frost. Test results have shown high efficiency of preparation in the field and in the film unheated greenhouse. Treatment helps to balance the negative impact of low temperatures and obtain an additional seeds yield 92-95 kg / ha more compared with the control, due to the increase of seed of productivity of plants to 3.2 g
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WATER QUALITY ASSESSMENT OF THE WATER ENVIRONMENT AND BIOTA IN THE VILUY REGION
DescriptionNumerous revealed negative impacts of the diamond industry and Vilyuiskaya HPP on the aquatic environment and biological objects are more expressed in the change of the hydrochemical regime in water basin of Viluy and as its consequence - in violation of the structural and functional nature of the components of aquatic biota; in the change of he average biomass and abundance of planktonic populations (phytoplankton and zooplankton) and benthic organisms, and in general - in the change of the flow energy in an aquatic ecosystem
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INTRODUCTION OF NEW GRAPES VARIETIES TO THE IN VITRO CULTURE
DescriptionClonal micro-multiplication – is this the basic method of obtaining the qualitative virus-free planting material. The high quality specificity of the explants reaction of the grapes varieties to the composition of nutrient medium requires the individual selection of the medium components for the most successful multiplication in vitro. In the article we present the results of studies on the cultivation of the grapes apexes in vitro the modified medium with the reduced content of macrocells. As a result of conducted investigations we have established that for the grapes varieties of Academic Trubilin, Artemis, Gurman Kraynova, Maria Kallas, Nizina, Petit Verdo and Traminer Black the most effective introduction into the in vitro culture (acclimatization of apexes 80- 100%) occurs on modified nutrient medium to Murasige and Skoog (1962), by being differed in terms of the content macro-elements (mG/l): NH4NO3 – 1237; KNO3 – 1425; MgSO4 · 7N2O – 277,5; KN2PO4 · H2O – 277,5, of the vitamin Of v1 – 10,0 mG/l, nicotinic acid – 4 mG/l. Remaining varieties of Kishmish luchistiy, Preobrajenie, Roshfor K and Yubiley Novocherkasska in the same medium were developed slowly and in a different way
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Description
The article presents the results of the studies on the variability of morphological traits of the cenopopulations of Allium prostratum, Allium ramosum on the natural territory of the Yakutsk Botanical Garden and in cultura. We have performed researches of biometric parameters, which revealed the structural features of the plant and the nature of the variability of their parts. The article also gives an estimation of coenopopulations vitality