03.00.00 Biological sciences
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VISUAL PHENOTYPING IN PLANT BREEDING
DescriptionThe article is devoted to visual analysis and its application in the selection process. New possibilities of application of visual display of information in the research process are considered. We have presented the examples of the use of visual phenotyping in breeding, evaluated the advantages and disadvantages in this area. In particular, the algorithm of the program of LHDetect for estimating leaf pubescence of wheat wafers is described, based on the analysis of digital images of the sheet bend. We have also considered Phenotiki platform that uses digital images of the profile of plants for visual phenotyping to assess plant growth. Based on the analysis of various studies it was noted that the application of visual analysis for some purposes is very effective, but for others, on the contrary, not effective. We highlight the actual problems of proper representation and converting information. The article shows the various causes of loss and distortion of data, as well as possible solutions to these problems. The work describes the application of ASK-analysis in the selection process. As an example, it describes the evaluation of the polymorphism of variety groups of grapes. We have presented interesting solutions and ways to minimize distortion and loss of information in the process of its transformation found by the authors of these works. The article reveals the concept of "computational biology" and describes fundamental differences from other areas as well as tasks, problems, possible research areas and promising directions of development of computer biology
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Description
The work was done in the research laboratory of the department of Biotechnology, Biochemistry and Biophysics of Kuban State Agrarian University, the aim of which was to select the most appropriate nutrient medium for maximum growth of lactic acid microorganism growth. The object of the study was its own microflora of gastrointestinal tract of quails ‒ Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius. As a nutrient substrate there were used 4 of the nutrient media: the medium for lactic acid bacteria (city Uglich), the melasse-autolysis medium, the glucose-peptone medium and the MRS. During the cultivation of the microflora of the variable parameters were the time and temperature of cultivation. During the cultivation of microflora the time and the temperature of growing were the variable parameters. In the process of growing there was carried out the analysis of dynamics of consumption of reducing substances and titers of microorganisms. According to the results of growing of microbial cultures there was revealed the active consumption of carbon substrate in used variants of nutrient media, and it was found that there was observed the maximum of cells to 24 h of cultivation. On the basis of carried out results of cultivation on different media and at different parameters there was determined that the most titer of cells was reached to 24 h at the temperature 38,0 °С on the melasse-autolysis medium. So, the melasse-autolysis medium can be recommended in production conditions as the cheaper substrate at the further development of biological preparations for poultry breeding
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Description
The article gives the description and the algorithm of the simulation model SMDS "Potok" 1.0, designed to calculate the parameters of distribution and sedimentation of technological alluviums in watercourses. The relevance of the simulation model is due to the need to determine the integral indicators (the volume of water flowing through the areas of the plume with a predetermined suspension concentration, the average layer of the silt, the specific density of the bottom silt in the calculated zone) when calculating the harm to water biological resources from the implementation of various economic activities in accordance with the methodology of calculation of damage caused to water biological resources approved by the order of the Federal Agency for Fisheries No. 1166 of 25.11.2011. Usage of the simulation model of SMDS "Potok" 1.0 will increase the reliability of calculations in determining the damage to water biological resources, as well as bring the calculations of parameters of distribution and sedimentation of technological alluviums in watercourses in accordance with the current regulatory legal acts of the Federal Agency for Fisheries
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BIOLOGY OF SEED GERMINATION OF SOME SPECIES OF FAM. ROSACEAE JUSS
DescriptionWe have studied the germination and the nature of germination of seeds of 20 species of FAM. Rosaceae. A wide range of germination characterizes their seeds: rapid, slow, with very poor or no germination. Laboratory germination of seeds stored at room temperature for 6-7 months have 80-100% - 8 species, 66-77% - 3, 1-9% - 4 species and 5 species did not germinate at room temperature, they need special conditions for germination
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Description
The article presents the data on vitality and age structure of Beckmannia syzigachne and Calamagrostis langsdorffii соеnopopulations under conditions of the Lena-Viluy interfluve. The study revealed 3 types of vitality of Beckmannia syzigachne (prosperous, equilibrium, and depressive), 2 types of vitality of Calamagrostis langsdorffii (prosperous and depressive). The age spectrum in both species is normal, non-complete, left-sided. According to the delta-omega classification, the coenopopulations are considered to be young
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INVESTIGATION OF TRACE ELEMENT COMPOSITION OF RAW STEVIA REBAUDIANA BERTONI MATERIALS
DescriptionThe article gives an analysis of the elemental composition in raw materials of stevia. Stevia plant is able to regulate the flow of heavy metals, allowing them, on the one hand, actively oppose the excessive intake of toxic elements and on the other hand, selectively cumulate essential elements. The contents of essential elements in plant raw materials do not exceed the acceptable standards regardless of the growing conditions
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Description
The author describes the dynamics of commercial catches and the forecasts of pikeperch catch in 2010- 2016 with an analysis of the causes that affect the state of stocks. The analysis of the effectiveness of the recommended pikeperch catch has been made taking into account the factors that affected the fishery. Researches were carried out to study the population structure, seasonal distribution, migrations and other characteristics of the pikeperch populations in the Azov limans. The obtained data made it possible to assess the state of the pikeperch stocks and give recommendations on their rational use. Based on the results of ichthyological studies, the pikeperch age, length and weight composition have been determined and its biological state has been studied. The preliminary forecast of the stocks and the recommended pikeperch catch in the Azov limans for 2018 is given. The forecast of the recommended pikeperch catch in the Azov limans for 2018 is based on the analysis of the stocks of commercial fish species for a number of years of observations, taking into account the total mortality rate under the influence of the fishery load (commercial mortality coefficient) and the amount of replenishment of commercial population with fish mature for the first time
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Description
The article gives the results of the study of an effect of mechanochemical powder of wood green of Abies (MPA) on potato morphogenesis in vitro. The study involved the hormone-free nutrient mediums (MS, ½ B5, ½ LS) for growing microcuttings from preliminary obtained aseptic plants. Plant morphogenesis was observed on the 15th and 30th days. At that, the number of developed shoots, plant height, leaves number, rhizogenesis rates, root number and length were estimated. MPA appeared to have the regulatory effect on morphogenesis of potato microcuttings. Based on mineral composition of the nutrient agar medium for potato microcuttings growing, MPA at 100 mg/l concentration induced root development (½ B5 medium), reduced height of shoots (MS medium), while the number of leaf blades was not affected
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COENOPOPULATION STATE OF SOME FABACEAE SPECIES UNDER CONDITIONS OF THE MIDDLE LENA RIVER VALLEY
DescriptionThe study concerns the results of the study of coenopopulations of the following Fabaceae species: Medicago falcata, Oxytropis candicans, and Onobrychis arenaria under conditions of the Middle Lena River. The study was conducted in Yakutsk vicinities, 13 km north-east of the city. It is stated that all the studied coenopopulations are normal. Rather small values of substitution and recovery indices evidence certain problems in self-maintaining processes. The age structure analysis using two age indices, “delta” and “omega”, allows to classify the coenopopulations of Medicago falcata, Oxytropis candicans and Onobrychis arenaria in phytocoenoses of the Lena River valley as normal young populations. They were prevailed by juvenile, immature, virginile, and young reproductive plants
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Description
We have presented information about species diversity of the coastal zoobenthos in some of Karasun lakes, Krasnodar. We have shown methodology information and reported the research area. We have made a taxonomic analysis. The biomass and the quantity of the main zoobenthos groups were calculated. Based on the two main fodder objects - Olygochaeta and Chironomidae and the P/B coefficient, there was calculated zoobenthos production of the studied lakes. The potential fish capacity was defined