03.00.00 Biological sciences
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INFLUENCE OF COMPLEX COMPOSTS ON DEVELOPMENT OF LIVING ORGANISMS IN TOP LAYERS OF ORDINARY CHERNOZEM
DescriptionComplex compost, which is formed in the process of unification of organic and mineral colloids, the formation of new cycles of nutrients, increasing the enzymatic activity of organic matter and respiration of living organisms, the formation of gaseous compounds, increase of ecological niches and land reclamation is a new direction in practical ecology, agriculture and soil science. This line determines of creation and use of complex mixtures of different wastes household, industrial and agricultural production for enrichment soil of organic and mineral dispersed and colloidal systems and improving their physical, chemical and biological and ecological functions. Formation of complex of compost is provided by the enrichment of substrate of organic matter, comprehensive set of mineral substances and their mixed colloidal composition. Microbial communities are determined by the level of transformation of organic matter and activity of life support system and the variety of living species. Entering into the top layer of soil of complex compost contributes to the development and expansion of ecological niches. At the complex compost it is very active increases the number of actinomycetes, especially representatives of the genus Streptomyces, the population of which are accumulated in the top layer of soil in the summer. Actinomycetes series of Cinereus were encountered most often in the soil after introduction of complex compost, Violaceus and Aureus were allocated less
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Description
In the Southern Federal University on the genetic basis of sunflower inbred line 3629, a collection of plastid mutants with varying degrees of chlorophyll deficiency was created by inducing Nnitrosomethylurea. Chlorophyll content was associated with their photosynthetic activity. It was found that the lower the chlorophylls content in mutant plastids, the lower the sugar concentration in plant tissues. For example, during the entire period of plant growth for whites (1.0-3.0 % chlorophylls a+b from control) var- 10, var-17 and yellows (6.0-9.5 % chlorophylls a+b from control) var-29, var-33 leaf areas of variegated mutants depending on the development phase and the content of green pigments are characterized by a low (2-7 fold) sugar content. Yellow-green (75.5% chlorophylls a + b from control) leaves of en:chlorina- 7 contain a higher level of carbohydrates, although it is 1.5-2 fold lower than at line 3629. Monosaccharides are products of hydrolytic reactions catalyzed by β- glycosidases. It was shown, that the activity level of β- galactosidase and β-glucosidase exceeded the corresponding indicators of the control at 1.5-2 and 2-7 fold, respectively. Similarly to enzymes from the water-soluble fraction, membrane-bound β- glycosidases also showed increased activity in the leaves of the investigated mutants, compared to the control green plants of 3629. Consequently, the activity of β-glycosidases increases dramatically in leaf tissues with deficiency of photosynthetic. Thus, chlorophyll mutations can lead to a change in the expression of nuclear genes, resulting in a significant increase in the activity of β-glycosidases in the mutant organelles themselves
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THE USING LOCAL NATURAL ORIGIN FORAGE RESOURCES IN COMPOUNDS OF BROILER CHICKENS
DescriptionUsing of compound feeds in broiler feed additives of plant resources, activates body's biological functions. This increases productivity and gives an ability to make environmentally friendly products. These local plants feed additives contributing to enrich animal feed with active ingredients include flour from nettle and Caspian marine algae. This article is devoted to studying the composition of nettle from the different locations of growth in Dagestan Republic and also, to determine the effectiveness of the use of nettle flour individually and to how implement it with the seaweeds flour in feeds of broiler chickens instead of herbal flour. The studies showed that nettle from a foothill zone in the flowering period exceeds slightly the nettle from mountain zone on the content of dry matter-1.19 % of protein-0.09 % and exchange energy 0.77 kcal/100g. Nettle meal from mountainous zone also inferiors the foothills in the energy value to 6.81 kcal/100 g. The inclusion of 2% feed meal nettle, 3% seaweed either alone or in combination of 2% instead of+3% of alfalfa grass meal resulted increasing broilers’ body weight 4,60-8,95% betterment survival to2,8-5,70%, increasing muscle output at 0,56-2,54% compared with the control group. The protein content and the amount of essential amino acids in the thoracic muscle in the test group was to 0.97-2.74%andto1,68- 2,11%, respectively, higher than the control. There was a significant increasing iodine in thoracic muscles of experimental groups 119,6-166,07% relative to control. Based on the results of the research, it is recommended to include 2% of feed meal nettle and 3% from algae instead of 4% grass meal of alfalfa to improve the productivity and biological value of broiler meat and 1 % of cereals
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SPECIES COMPOSITION OF ALGOFLORA IN AGROCENOSIS OF KUBAN
DescriptionSpecies composition and abundance of soil algal flora were studied in the Central zone of the Krasnodar region on crops of crops cultivated by various technologies. In the algal flora of the studied area, 48 species of soil algae and cyanobacteria from 28 genera and 18 families, 7 classes and 4 divisions were identified. The largest and numerous in number of species are the departments: Cyanophyta and Chlorophyta. Extensive delivery: Oscillatoria, Gloeocapsa. They account for 22 % of the total number of species. A negative effect of herbicides on number and species composition of soil algae was noted. In all variants of their application there was no form of Klebsormidium flaccidum (Kützing). The number of species of Chlorella vulgaris Beyer. was low, Botrydiopsis arhiza Borzi. It is believed that these species of algae can be used as bioindicators. The positive effect of mineral fertilizers and the aftereffect of introducing organic matter on the soil algal flora have been established. In these variants the deleterious effect of herbicides was reduced. According to the calculated regression equations, the applied mineral fertilizers to a greater extent weakened the negative effect of herbicides on the total number of populations than the aftereffect of introduced organics
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DETERMINATION OF ACUTE TOXICITY OF BIO HUMATES
DescriptionIn the work, we present the results of the investigation of acute toxicity of bio humates obtained by the method vermicultivation on laboratory animals. In the diets of farm animals and Pets to fill in the missing nutrients in the past years, we had an extensive use of various feed additives. Among them, mineral (macro - and micronutrients), protein and fat supplements, vitamins, biostimulants, complex natural compounds (sapropel, peat), synthetic products (enzymes, hormones, antibiotics, adaptogens, antioxidants). The search for new ways of improvement and increase of efficiency of agricultural animals using feed additives with high demands on ecology of meat and dairy foods naturally led to increased studies on the use in livestock farming of water-soluble alkaline salts of natural humic acids - humates. Their environmental safety and the unique ability to improve metabolism and increase energy cells very positively manifested in living organisms. Numerous studies Russian and foreign scientists installed a high efficiency natural humates as biostimulators and immunomodulators in animal husbandry and veterinary medicine. Accumulated extensive experimental material, proving that the use of humates leads to faster growth of animals, reducing morbidity and mortality, increase the body's resistance to toxins in feed and resistance to adverse environmental conditions. Improving with the help of humates ecological purity of the ecosystems "water — soil — plant", as well as the health of birds, animals and fish will ultimately lead to strengthen health and to prolong human life as a consumer of agricultural products. Currently, the livestock market is intensively increasing the humates produced in Russia and abroad from brown coal, peat, sapropel. A special place among them has been occupied by the newest formulations because of a unique combination of safety, efficiency, and value, which, thanks to its amazing properties to increase energy cells, stimulate vital processes and to enhance the beneficial effects of other substances called energine. They are harmless to animals and humans; do not have allergologie, anaphylactogenic, teratogenic, embryotoxic and carcinogenic properties when used in recommended doses. This allows you to create on their basis organic natural feed additives and veterinary medicines for agricultural animals, birds, fish, fur animals and Pets, and preventive and therapeutic drugs for humans
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Description
Industrial horticulture assumes the most effective use of the potential of varieties. One of the key factors determining the yield of garden plantings is the effectiveness of pollination. To obtain the maximum yield, it is necessary to ensure maximum pollination during the flowering period. For this reason, much attention has been paid to the selection of pollinators. Crab-apple forms are promising for use as pollinators, so this work was aimed at identifying the most common alleles of the self-incompatibility gene in the crab-forms using the molecular genetic method of analysis. The object of the study was 29 apple-tree creams and 3 elite selection forms. They carried out the molecular genetic identification of alleles S2 and S10, which are among the most common apple trees in the world gene pool. Allele S2 was identified in 16 samples (14 forms and 2 elite selection forms), while S10 allele in one sample (elite form 12/2-20 (24-28)). Data on the allelic composition of the S gene in the samples studied are of value for the formation of a genetic passport on the compatibility of the studied samples of apple with modern industrial varieties
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Description
The article presents the long-term data (2007-2015.) biometric aboveground plant parts and the estimation of the prospects of the three introduced botanical species of juniper and recommendations for their use in landscape design. We have established very promising, promising and unpromising varieties for landscaping in the conditions of the foothill zone of the Krasnodar region out of 16 varieties of juniper species: China, ordinary and rock
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NUTRIENT MEDIUM FOR INDUCTION GEMMOGENESIS TO CREATE SOMATIC CLONES OF TEA PLANTS IN VITRO CULTURE
DescriptionThe article presents the first results of studies of somaclonal variability, which take place during cultivation of tea plants tissues and organs in vitro culture. As a starting material, there was used morphogenic callus, isolated from the basal part of tea microshoots, because callus increases somaclonal variability. An optimized protocol of the nutrient medium for induction gemmogenesis to product somatic clones of tea plants in vitro culture was developed. Morphogenesis calluses, initiated from the basal part of tea microshoots and subcultured on the nutrient medium with 6 – BAP – 2,5 ml + gibberellic acid – 1,0 ml + Tidiazuron – 4,0 ml + Tryptophan – 1000 mg, distinguished by high rates of induction of gemmogenesis – 63,3 %. The article studies the effect of exogenous growth regulators on morphological and growth indices of tea callus culture in vitro
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PROSPECTS OF CULTURE SPECIES OF BETULACEAE IN ROSTOV REGION
DescriptionWere summed up of perennial introductory tests of the representatives of the family Betulaceae S.F. Gray in the Botanical Garden SFedU. The 33 species of this family have been studied for a total. Evaluation of winter hardiness of the family members showed that the absolute resistance to the full range of factors winter have representatives genus Betula, also Alnus incana (L.) Moench and Corylus colurna L. Species of the genus Betula and Corylus may be attributed to the plants having a resistance to drought. The highly drought-resistant species – Carpinus betulus L., C. turczaninowii Hance, Alnus incana (L.) Moench and Ostrya carpinifolia Scop. Less than drought-resistant – Alnus glutinosa (L.) Gaertn. Most of the tested species should be attributed to hemixerophytes. The high degree of defeat by fungal diseases of the species genera Alnus, Betula and Carpinus makes them unsuitable for the regional culture. The causative agent of the disease is a fungus Chondrostereum purpureum (Pers.) Pouzar. Based on the totality ecological and biological properties only species of the genus Corylus remain promising for the culture. For introductory test it is advisable to attract drought-tolerant species of the family Betulaceae such as: Alnus barbata C.A. Mey., A. rubra Bong., A. rugosa (Du Roi) Spreng., Carpinus cordata Blume, C. caucasica Grossh., C. caroliniana Walter, C. orientalis Miller., C. tschonoskii Maxim., Betula insignis Franch., B. lanata (Regel) V.N. Vassil., B. luminifera H.J.P. Winkl., B. nikoensis Koidz., B. forrestii Hand.-Mazz., B. utilis D. Don., Ostrya virginiana K.Koch
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BIOMONITORING OF CHEMICAL ELEMENTS CONTAMINATION OF RECREATIONAL AREAS OF ROSTOV-ON-DON
DescriptionThe article presents the data on the chemical elements content in soils, plant leaves and lichens of three squares in Rostov-on-Don. The calculation of the total index of soil contamination by chemical elements finds that soils of the square are characterized by a low degree of contamination