16.00.00 Veterinary sciences
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CLINICAL PHARMACOLOGY OF A NEW COMPLEX HEPATOPROTECTIVE PREPARATION
DescriptionThis article presents the results of the studies of the influence of a new complex hepatoprotective preparation on the basic system of the body, the mechanism of occurrence and manifestation of its biological effects, the dependence of this action from the components that are parts of the preparation, the dose, as well as the regularity of manifestation of possible side effects. The effect of the different doses of the preparation (1% and 2%) on the average daily weight gain and morphological and biochemical indices of the birds’ blood was studied. The conducted research determined a stimulatory influence of the preparation on the growth, development and safety of broiler chickens. The new hepatoprotector exhibits the properties aimed on revitalizing the erythro- and hematopoiesis and magnification of the cellular immunity against the exogenous influence. The use of the preparation helps to improve liver function and reduce the toxic load on hepatocytes, which manifests an increase in a number of metabolic parameters, such as total protein, glucose, calcium, phosphorus. We have noted an expressed hepatoprotective effect on the enzyme activity of AST and the remission of the cytolytic syndrome of the experimental chickens. Thereby it was found out that the complex hepatoprotective preparation has a pronounced pharmacological activity, providing a significant impact on the energy of the broiler chickens’ growth and their safety, morphological and biochemical indices of the blood and metabolic processes in the body of the bird
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Description
The article presents the use of intensive methods of diet herd reproduction that will increase the productivity and profitability of the sheep industry. One of such methods is in vitro fertilization (IVF). For the success of in vitro fertilization procedure, an important requirement is the availability of highquality nutrient media, which help to preserve the genetic material and contribute to the further development of the zygote. The main requirement to the media for sperm is the ability of media not to cause their agglutination. The aim of our work was to search for new ways to reduce the agglutination of spermatozoa in the preparation of freshly prepared sperm in the process of production of embryos in vitro. To eliminate the agglutination of spermatozoa in the semen preparation stage, we used GCY medium, followed by demolition of seed in SOFw environment, which has resulted in a significant (almost 15 times!) reduce of the number of bound sperm. In our opinion, a decrease in agglutination in GCY buffer was connected with a specific influence of the constituent components of sperm. Conclusion: Thus, our method of preparation of freshly prepared sperm for in vitro fertilization allows a sharp decline in sperm agglutination, which will improve the fertility of eggs during the production of embryos in vitro sheep
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Description
The level of development of modern medical equipment for the past 20-30 years is in constant development, many of the technologies and methods of treatment of certain diseases that were previously only available for the treatment of a person with success began to penetrate in the veterinary practice. The objective aim at the initial stage was the establishment of testing and suitability for the cultivation of autologous dermal fibroblasts animals of previously known techniques and the modernization of the existing techniques. Next, we had to carry out measurements of morphometric parameters and to identify structural features and functional activity of fibroblasts of different kinds of agricultural animals. Conclusions: The study showed the conducted morphometric autologous dermal fibroblasts tests allowed us to obtain the correlation line. In addition, to identify common patterns in the development and growth of fibroblasts derived from different species of agricultural animals. And also, to obtain material for comparative evaluation of the quality of the obtained cell cultures using entropy equivalent
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Description
According to the title, the article describes the causes and contributing factors of functional disorders of the uterus and ovaries at cows. The authors specified the percentage incidence of these animals’ pathologies in some farms of Krasnodar Region, especially in industrial complexes. Studies have shown that acute postpartum endometritis has a definite influence on the functional disorders of the ovaries at cows. Authors have found that the cows via 2-4 months after calving with ovarian hypofunction in early postnatal period have had purulent-catarrhal endometritis in 87.9%, fibrinous - in 12% of cases. At cows which have had persistent corpus luteum in 54.6% of cases was registered purulent-catarrhal endometritis, in 27.6% - fibrinous, in 17.8% - necrotizing metritis. At cows with ovarian cysts fibrinous endometritis was observed in 63.2% of cases, necrotizing metritis - in 36.7%. Thus, the more severe form of uterine inflammation was observed in cows, the more severe form of ovarian functional disorders observed in them. The authors found that any factors which negatively acting on the central nervous system or hormone levels, directly or indirectly, sharply reduce the reproductive function of animals
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FACTORS CONTRIBUTING TO THE EMERGENCE OF FUNCTIONAL DISORDERS OF BIRTH-GENITAL APPARATUS AT COWS
DescriptionЖИВОТНОВОДСТВО, КРУПНЫЙ РОГАТЫЙ СКОТ, ДИСФУНКЦИЯ ЯИЧНИКОВ, ЭНДОМЕТРИТ, БЕСПЛОДИЕ, СУБИНВОЛЮЦИЯ МАТКИ, ПОЛОВОЙ ЦИКЛ, ПЕРСИСТИРУЮЩЕЕ ЖЕЛТОЕ ТЕЛО, КИСТА ЯИЧНИКОВ, ГОРМОНЫ
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DETERMINATION OF ACUTE TOXICITY OF ROKSATSIN
DescriptionIn this article, the authors have defined the acute toxicity of Roksatsin preparation, which represents a 20 % aqueous solution of polyhexamethylene guanidinehydrochloride (PHMG). The acute toxicity of Roksatsin in the first series of experiments was studied on white mice upon intragastric administration. In the second series of the experiments, Roksatsin was administered subcutaneously to white rats and in the third series of experiments the preparation was administered intracisternally to cows. The results of the first series of experiments showed that Roksatsin by the internal method of appointment is slightly toxic to white mice. In this experiment not been a single case of acute intoxication and death of animals. Experiments to determine the acute toxicity of the preparation for white rats were performed in triplicate. In the first series of experiment, the LD50 was 4.8 ml/kg. It is found, that the calculated coefficients LD50 for other two series were close to the first one: the second - 4.2 ml/kg and the third - 4.5 ml/kg. Therefore, the authors found that the Roksatsin preparation upon subcutaneously administration according GOST 12.1.007-76 is a low-toxic compound (4th class of danger). As a result of the third series of the experiments, it was found that the intracisternally administration of Roksatsin preparation to cows in a dose of 5 ml is not toxic to the animal organism. Intracisternal administration of Roksatsin does not have a significant impact on the morphological and biochemical indices of blood. On this basis, Roksatsin preparation with different routes of administration is low-toxic and it can be recommended for clinical researches
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EFFICIENCY OF ROKSATSIN IN AEROSOL DISINFECTION OF THE LIVESTOCK BUILDINGS
DescriptionThe authors briefly describe the properties of polyhexamethyleneguanidine hydrochloride (PHMG), which refers to a broad-spectrum biocide and has antimicrobial activity against Gram-negative and Gram-positive bacteria, viruses and fungi. PHMG has a deodorizing effect, gives the treated surfaces long bactericidal effect, which can be stored depending on the surface and other external factors from 3 days to 8 months. The authors have presented data about the level of bacterial and fungal contamination of air in the dispensary before and after aerosol treatment of Roksatsin. Bacterial contamination of air dispensary determined via the sedimentation method (Koch Method), which is settling microflora (in air), under gravity, on the surface of a growth medium. For the determination of total bacteria and fungi in 1m3 of air the authors make calculations of total aerobic microbial count (TAMC) according to the formula that was proposed by V.L. Omelyanskii. Bacterial contamination of air was evaluated before disinfection. Accounting quality of aerosol disinfection performed by sedimentation microflora on Petri Dishes through 30, 60 and 120 minutes of exposition. In the analysis of the data the authors defined that Roksatsin as a disinfectant has a negative effect on pathogens, namely significantly reduced the content of pathogenic and conditionally pathogenic bacteria and fungi in the air, so it can be used for preventive and compelled aerosol disinfection of air in the livestock buildings
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THE RESULTS OF IMPLEMENTATION OF CATTLE EMBRYOS TRANSPLANTATION
DescriptionThe results of the analysis of the level of implementation of the method of cattle breeding by embryo transfer in an agricultural company from the Krasnodar region with a developed sector of cattle breeding. We have assessed the level of development of all stages of the method in the household, identified errors in the selection of animals and the increased value of culling donor cows and recipients, reduced duration of using recipient cows and high cost of all phases of the transplant process. We have raised a question of the need to change the criteria of economic evaluation of the results of the method
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Description
The article contains a synthesis of modern views on the problem of ketosis and related metabolic disorders. We have given evidences of the exceptional role of hypoglycemia in the development of progressive metabolic diseases of cows during the transition period. The necessity of implementing a program for critical period with mandatory control of glycemia was substantiated
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THE FACTORS PROMOTING DISTRIBUTION AND UROLITIAZ'S DEVELOPMENT IN CATS
DescriptionThe authors were conducting researches on 36 unhealthy cats in a veterinary clinic in Orenburg, from 2014 till 2016, and they were diagnosed the urolithiasis. By the results of the researches, the influence of a season of year, age and breed on distribution and development of an urolithiasis disease in cats has been studied. At the same time, it is established that the urolithiasis disease usually occurs at cats from 2 up to 6 years, which makes 45,0% of all the studied animals. A disease outbreak at cats was observed during summer and autumn periods, generally, not purebred animals - 75% are subject to the disease, the rest 25% is such breeds as the British, Persian, Bengalese, Scottish; in addition, the influence of an unbalanced diet, non-compliance with the norms and the mode of feeding is noted