06.01.00 Agronomy
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MAGNETIC PROFILES OF KUBAN SOILS OF VARIOUS AGRICULTURAL USE
DescriptionThe article discusses the change in the magnetic properties of soils of different agricultural use, depending on the conditions of soil formation. The objects of study are leached chernozem of Western Ciscaucasia, meadow chernozem and meadow bog soils of rice agrolandscapes. The results showed that with an increase in the degree of hydromorphism, the magnitude of the magnetic susceptibility of the soil decreases. The leached chernozem has the highest magnetization of the profile. In soils formed under the influence of hydromorphic soil formation, magnetic susceptibility is reduced by 3.0-3.5 times in rainfed conditions and deposits, and by 6.0-12.0 times in soils of rice agrocenoses
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THE MECHANISM OF STABILITY MANAGE-MENT OF THE REPRODUCTIVE PROCESSES IN THE VITICULTURE
DescriptionAn organizational and economic mechanism for managing functional stability has been developed, the components of which are optimal structural and regulatory decisions, as well as a calculated and reasonable regulatory framework as tools for ensuring the sustainability of reproduction processes in a mode that ensures achievement of output parameters guaranteeing a given level of efficiency. The dimension of regulators is calculated based on ensuring the sustainability of reproduction processes in industry production
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Description
This article discusses the results of research on the effect of soil treatment on agro-physical performance and productivity of major field crops. The task of the research was to determine the impact of primary tillage (moldboard plowing), surface treatment (minimum) and zero (direct seeding) is factor A, fertilizers – B, and herbicides as factor C for agro-physical properties of soil. We have found that high density led to a significant increase in the proportion of the stone faction in the structural composition of the soil. Deterioration of the agro-physical properties while minimizing soil tillage has negatively affected water, air and food regimes under crops rotation. When all methods of soil tillage are applied, the moisture reserves in the 0-200 cm layer in early spring had the lowest values of moisture-holding capacity (230-272 mm) and were defined by the features of weather conditions and past cultures. At the time of sowing, their number decreased by 12% (203-241 mm). The most economical way of spending moisture was shown by plants on the moldboard tillage. In autumn, fewer weeds (7-11 PCs/m2) there were in the options for ploughing. When the surface and zero ways of soil treatments were applied, their number for 1 m2 was 1.2-1.9 times bigger. We effectively eliminated weeds only when applying herbicides. Different crop cultures reacted differently to minimizing the tillage. The greatest reduction in yield was observed when applied surface and zero tillage in cultivated crops. Thus, studies have shown that in order to obtain maximum yields of field crops of crop rotation, reducing production costs, there must be a rational combination of deep or conventional plough tillage for surface or zero tilled for grain crops
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Description
The article deals with the realization of the re-productive potential of wheat with the use of multi-flower formation as a model object of winter soft wheat plants. The essence of the concept of "reproductive potential" is disclosed, as well as the significance of this concept for research aimed at increasing the productivity of wheat plants. The data obtained during the study and analysis of varieties of winter soft wheat with the sign of multi-flower formation are given. The reproductive potential of winter soft wheat plants was determined by counting flowers at the VI stage of organogenesis. The examined multi-flower forms of soft winter wheat compared with Krasnodar breeding varieties showed increased reproductive potential, as well as an increased number of grains with the ear, but with a reduced mass of 1000 grains. According to the data obtained, the degree of the reproductive potential of multi-flower forms of winter soft wheat varied significantly, depending on the weather and climate conditions of the year of the study. In conclusion, the choice of multi-flower formation of winter soft wheat as a model object for investigating the reproductive potential of wheat, as well as the factors that influence its implementation and the extent of such influence is justified
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A MODIFIED PROTOCOL OF RNA ISOLATION FROM MATURE LEAVES OF GRAPES FOR RT-PCR
DescriptionIsolation of high-quality RNA from the tissues of perennial woody plants, including woody grape vines, is very difficult due to the high content of phenolic compounds, secondary metabolites and polysaccharides and the ribonuclease activity of destroyed tissues. Most of the existing methods require either large time or financial costs, or do not give reproducible results in the case of RNA extraction from mature grape tissues. The modified isolation protocol is based on a combination and modification of the known RNA extraction methods, taking into account the characteristics of mature grape tissues. Existing commercial kits for the isolation of RNA from plant tissues showed a low efficiency of RNA extraction from mature grape tissues, primarily associated with "varietal specificity". Reproducible results in the extraction of RNA showed CTAB-method, however, it has several significant drawbacks associated with the duration of the extraction and the complexity of the processing of an RNA preparation with a DNAase. The developed method is based on increasing the concentration of mercaptoethanol and polyvinylpyrrolidone in the extraction buffer, eliminating the stage of RNA selective precipitation via LiCl, and replacing it with deposition on a silica-based membrane (SiO2) followed by processing with DNA-ase. and increase the purity of the preparation of RNA from genomic DNA in comparison with the original method. A modified isolation protocol was developed based on a combination and modification of known RNA extraction methods, taking into account the characteristics of mature grape tissues. This solution allows to obtain reproducible quantity and quality of RNA for the subsequent synthesis of cDNA and RT-PCR
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MOLECULAR GENETIC ASPECTS OF PERSIMMON (DIOSPYROS L.) BREEDING
DescriptionPersimmon is the most cold-resistant subtropical culture. It is relevant in scientific research and a prospect for the application of modern methods in plant breeding. The aim of this work is to review the latest advances in molecular genetic methods in persimmon breeding (Diospyros L.). The article shows the importance of studying the molecular genetic basis of persimmon breeding as a necessary condition for the development of new approaches in the production of new varieties. Statistics on the cultivation of this crop on an industrial scale is presented. The importance of culture is shown and given information on the territory of cultivation. Data on the study of genetic diversity, assessment of genetic stability, understanding the origin of persimmon varieties, degree of relationship using a number of molecular markers are presented. There are also DNA sequencing results, which are shown closely related relationships of Diospyros kaki with wild species D. oleifera, D. deyangensis, D. virginiana, D. glaucifolia, D. lotus and D. jinzaoshi. The reduce the astringency of the fruit, the significant role of regulation of structural genes (DkADH, DkPDC, DkPK, ALDH) in the biosynthesis of proanthocyanidins, in increasing the tolerance of plants to abiotic and biotic stress factors, level of expression of the studied genes are presented in this review
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Description
This overview and theoretical article deals with the consideration of hypothetical possibilities for the development of molecular-kinetic markers of agricultural plants allowing to quantify the effect of genotype-environment interaction on the basis of stability studies of mRNA. The development in view is based on the results of studies of the mRNA decay identity in vivo and in vitro (the ommp system), as well as the phenomenon of RNA interference (RNAi), widely studied in plants. The ommp system has allowed to establish the relationship of the cultivar-specific growth reaction to the effects of low positive temperatures, dehydration, salinity, illumination and biologically active substances with the stability of the total and a number of gene-specific mRNAs of green and etiolated seedlings of winter soft wheat (Triticum aestivum L.) and winter barley (Hordeum vulgare L.). Similar studies of mRNA stability have been conducted on ripening grains of maize (Zea mays L.), with a particular result of the in vivo and in vitro decay identity confirmed for major mRNAs of stored proteins, 19 and 22 kDa zeins, of normal maize and mutant according to the regulatory gene opaque-2, changing the amount and stability of zein mRNA in the maturing grain of high-lysine maize. Regulatory response of the organism through RNAi is also multiple and includes neutralization of viral and bacterial infections, reaction to pathogens and biologically active substances, circadian rhythms, water stress, hypoxia, mechanical stress, mineral nutrition, salt stress and temperature changes. Unfavorable environmental influences result in an increase or decrease in the expression of certain microRNAs (miRNAs). The change in mRNA stability is an important component of the gene expression regulation system in eukaryotic cells. The main determinants of mRNA stability are in the 3'-untranslated region. It is the It is the (U)nА sequence and the degree of polyadenylation of mRNA, i.e. length of its terminal homonucleotide chain. It is to this region that mRNA molecules are complementary to miRNA. The most important component, which largely determines the regularity of the genotype-environment interaction, is the polyadenyl sequence at the 3 'end of the mRNA. Its length depends both on the genotype and on the environmental conditions. There is evidence that polyadenylation degree of mRNA determines the secondary structure of the molecule. As is known, deadenylation of mRNA reduces its lifetime, and when the poly-A-tail reaches several dozens of nucleotides, an explosive disintegration of the mRNA molecule occurs. Consequently, in a living cell the following mRNA decomposition scheme appears to be logical: shortening the poly-A-tail of the mRNA opens the sites of miRNA interaction with the 3'-non-coding region of the mRNA molecule, which causes its degradation. Thus, it is safe to assume that the interaction of mRNA and miRNA in the ommp system is the underlying process for molecular-kinetic markers under development
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MORPHOLOGICAL FEATURES AND SOWING QUALITIES OF SILYBUM MARIANUM (L.) GAERTN. VARIETY
DescriptionDistinctive features of seeds of Silybum marianum two varieties – the ‘Start’ and the ‘Debut’ on morphology are presented in the article and also data on influence of storage conditions on sowing qualities of seeds are submitted. Seeds of two varieties differ among themselves on coloring, a form and mass of 1000 seeds. The ‘Debut’ is characteristic sign of a variety existence of dark strips on a surface of seeds. Results of two years of tests have shown that during 1 year from the moment of collecting seeds energy of germination and viability gradually increases and reaches the maximum values in 12 months independently of storage conditions. The viability of seeds of the Silybum stored at the room temperature within 18 months decreases by 8,5-12,5%. As a result of an experiment it has been established that in the conditions of not heated room successful storage of seeds is possible not longer than one year, after that sowing qualities of seeds sharply decrease
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INHERITANCE OF PRODUCTIVITY TRAITS IN RICE HYBRIDS
DescriptionGreat success in increasing rice production, and it has more than doubled, has occurred worldwide thanks to the use of modern high-yielding rice varieties developed by conventional breeding methods. In order to develop a new variety, it is important for the breeder to study the genetic and breeding value of various cultural and wild species - carriers of economically valuable traits, the identification of genes and groups of genes controlling the economically valuable traits, to study the patterns of their inheritance, to establish patterns of interaction between the genotype and the environment, explore the genetic and physiological-biochemical basis of the phenomenon of heterosis, improve methods of intraspecific, complex, stepwise and distant hybridization, to study a phenomenon of a sharp increase in the quantitative characteristics of crop plants, a more powerful growth of hybrids of the first generation compared with the parent individuals. Scientists of the past centuries, such as A.F. Wigman, W.Noden, V.Foke, and many others have been studying the phenomenon of hybrid power, which was first named “heterosis” in 1908 by J. Schell. At present, heterosis is understood as the phenomenon when hybrids of the first generation exceed their parental forms in terms of their biological values and economically valuable traits. The magnitude of heterosis of the first generation hybrids is determined by several methods in terms of percentages to the paternal, to the maternal form, to the average of both parents, and to a better or regionalized standard. By the magnitude of the dominance coefficient, researchers often determine the inheritance of a trait
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Description
The generalized long-term experience on creation of base of Queen cells in grape varieties, improved in vitro culture, on the sandy array of Ust-Donetsk district of Rostov region. Problems frequently encountered on sandy soils and ways of their solution are shown. We focus on: rehabilitation of the landing of plants of grapes in the conditions of greenhouses, open ground, the optimum time and methods of planting. The work shows the effectiveness of different dressings and types of fertilizers, influence of drugs of new generation, affected the uterine protection of plantations from pests and disease, deep sandy soils freezing in winter, especially the development of root system of different varieties. On the basis of generalization of long-term data, we suggest the most appropriate solutions, and developed technology bookmarks and conducting basic queen cells from healthy in vitro planting material of grapes in the sandy array