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Besedina Ekaterina Nikolaevna

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• State Scientific Organization North Caucasian Regional Research Institute of Horticulture and Viticulture of the Russian Academy of Agricultural Sciences
 
 

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• Buntsevich L. L. ¹

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• pdf  256.845kb doc 256.845kb Views: 1286 Date: 30.09.2015
  Besedina E. N. Buntsevich L. L.

  IMPROVEMENTS OF CLONAL MICROPROPAGATION TECHNOLOGY OF APPLE ROOTSTOCKS AT THE STAGE OF INTRODUCTION TO IN VITRO CULTURE

  06.00.00 Agricultural sciences

  Description

  The stage of introduction of plantlets to in vitro culture is an important stage of technology of clonal micropropagation of plants. For the purpose of decrease in a share of the plantlets that were lost from an infection and increase of their regeneration ability, sterilizers and antibiotics, effective and safe for apple rootstocks’ plantlets wee allocated, and also favorable terms of introduction to in vitro culture were allocated. As a result of the conducted researches, we have established that on influence on an nutrient medium and plantlets infection and on growth and development of plants, beneficial effect on apple rootstocks’ plantlets has an antibiotic nystatin of 200 mg/l: the reproduction coefficient on the medium with this antibiotic is equal 4,3, in the same concentration nystatin has the sanifying effect of 60-75% for stocks of SK 2 and MM 106. During researches of sterilizers for apple rootstocks’ plantlets SK 2, SK 3, SK 4, SK 7, MM 106 as an alternative to widely applied highly toxic sterilizer corrosive sublimate (the first class of danger) were picked up effective and safe preparations for sanitation of plantlets from an infection, such as the household preparation "Whiteness" (sodium hypochlorite) in cultivation 1:2, low-dangerous substance of the fourth class of danger (a share of viable plantlets of 75,5% from initially introduced), and also fosfopag, a preparation of the fourth class of danger (a share of viable plantlets of 65% from initially introduced). The favorable term for a meristem of apple rootstocks’ plantlets’ introduction to in vitro culture are phases of buds’ burgeoning (March) and the intensive growth of shoots (May – June)