name

Nikulin Nikita Alekseevich

Scholastic degree

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Academic rank

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Honorary rank

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Organization, job position

• Vyatka State University
  Институт биохимии и физиологии микроорганизмов им. Г. К. Скрябина РАН
 

Research interests

молекулярная биология, молекулярная генетика, биоинформатика, экология бактериофагов, молекулярные основы биоразнообразия

Web site url

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Email

nikitakulin@gmail.com

Current rating (overall rating of articles)

0

TOP5 co-authors

• Zimin A. A. ²
• Koshchayev A. G. ²
• Коnоnеnко S. I. ¹
• Suzina N. E. ¹

Articles count: 2

Сформировать список работ, опубликованных в Научном журнале КубГАУ

• pdf  269.286kb doc 269.286kb Views: 686 Date: 30.11.2017
  Nikulin N. A. Коnоnеnко S. I. Koshchayev A. G. Zimin A. A.

  DESIGN OF THERAPEUTIC PHAGE COCKTAILS BASED ON T4-TYPE BACTERIOPHAGES: ADVANTAGES AND DISADVANTAGES

  03.00.00 Biological sciences

  Description

  In the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy

• pdf  822.248kb doc 822.248kb Views: 412 Date: 31.05.2019
  Zimin A. A. Nikulin N. A. Koshchayev A. G. Suzina N. E.

  MORPHOLOGICAL FEATURES OF I-FORMS OF PF-RELATED BACTERIOPHAGES IN PSEUDOMONAS AERUGINOSA PAOI LYZATES BY EXTRACTS OF EUROPEAN BIZON’S FEKALIA

  06.02.00 Veterinary and Husbandry

  Description

  In this work, we have found shortened particle forms of the filamentous bacteriophage Pf, when the lysis of the Pseudomonas aeruginosa PAO1 strain was induced by extracts of bison feces to study the intestinal bacteriophages of this animal. The sizes of the shortened particles from 250 to 950 nm and their thickness 17 nm were determined. The particles had a slightly pointed one end and sometimes a flag on the other extended end. The particles were formed in the process lysis of this strain by other bacteriophages. These lysates also contained a high concentration of vesicles from the pseudomonad membrane. The article discusses the mechanisms of prophage induction in the Pseudomonas aeruginosa PAO1 strain due to phage-phage induction and substances of the investigated extract