Innovative University of Eurasia
Author list of organization
List of articles written by the authors of the organization
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OBTAINING DIAGNOSTIC KITS FOR SERODIAGNOSIS OF CARNIVOR INFECTIOUS HEPATITIS
Description
The article is devoted to the current problem of differential diagnosis of diseases of viral etiology in small pets. Viral diseases of dogs and cats are widely distributed among both pedigree and non-pedigree animals. The spread of diseases is facilitated by the increase in the number of small pets, the popularization of pet maintenance, cross-border operations associated with the movement of animals. The importance of express methods in laboratory diagnostics of infectious diseases is considered. Reducing the time of diagnosis contributes to the effectiveness of therapeutic and anti-epizootic measures. The place of infectious hepatitis of carnivores in the structure of morbidity of dogs is analyzed. The article describes the research in determining the optimal systems for the cultivation and subsequent isolation of the infectious canine hepatitis virus in high titers. If viral biomass needs to be increased, the choice of a cultivation system for maximum harvest is of paramount importance. The authors propose a method of obtaining and purification of viral antigens to use them for preparation of diagnostic kits. The release of the isolated fraction of ballast substances, which provides rapid response and strong binding to specific immunoglobulins is essential for the preparation of antigenic drugs. It is important to obtain antigens with a high degree of purification to get reliable results in the formulation of serological reactions
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