North-Caucasus Research Institute of Animal
Author list of organization
List of articles written by the authors of the organization
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EXPLORE THE POSSIBILITY OF GROWING YOUNG GEESE IN THE DIETS OF THE SEEDS OF RAPE OF 00-TYPE
06.00.00 Agricultural sciences
DescriptionThe article deals with the problem of growing geese and explores the possibility of feeding young geese with rapeseeds of 00-type and their products
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THE APPLICATION OF THE BACTERIOPHAGES TO COMBAT COLIBACILLOSIS AND CAMPYLOBACTERIOSIS IN POULTRY
DescriptionThis work considers the needs and methods of phage therapy of two bacterial infections in poultry. The review examines the use of bacteriophages, from the moment of their discovery to the present day. We discuss the relevance of such diseases as compilobacteriosis and colibacteriosis transmitted from birds to humans, we show how to use bacteriophages and their efficacy in models of adult birds and chickens, including information provided on the pilot study of phage therapy at a poultry farm. The reported study was partially supported by RFBR, research projects No. 16-44-230855 and No. 13-04- 00991 and № 16-44-230855-р_а
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ABOUT USING FAT ADDITIVES IN FEEDING OF AGRICULTURAL POULTRY
06.00.00 Agricultural sciences
DescriptionThe article is dedicated to the problem of full-value rations for farm animals and poultry at the expense of the use of different fat additives. We have also discussed the prospect of dry palm oil usage in geese feeding
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USE OF FAT ADDITIVES IN FEEDING OF AGRICULTURAL ANIMALS AND POULTRY
06.00.00 Agricultural sciences
DescriptionThe article is dedicated to the problem of different fat additives use in the rations for agricultural animals and poultry
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THE USE OF TRITICALE IN THE DIETS OF YOUNG GOSLINGS
06.00.00 Agricultural sciences
DescriptionThe article presents the results of studies on the use of different amounts by weight of triticale grain in feeding of young geese raised for meat. In the first group the wheat grain was 50% replaced by triticale grain, and in the second group the wheat was completely replaced by triticale grain. As a result of the experiment it has been found that the replacement of wheat by triticale in complete feed for geese provides a high growth rate but at 50 % inclusion of triticale grain the live weight at the end of the experience was110.8 g or 3% higher, as compared with the second group. It should be noted that up to 49 days old geese receiving compound feed with the 100 % replacement by triticale grain had a higher body weight. Complete replacement by triticale grain favoured the increase of carcass yield by 2.2% and decrease of body fat by 68.7 g as compared with the first group. Muscle tissue of the goslings fed the complete feed with complete replacement for triticale grain was characterized by 1% lower caloriс content than the goslings of the second group. According to biochemical composition of blood, all designed rations ensured normal metabolism in the fattened geese. The inclusion of triticale grain into the complete feed for young geese reduced their costs in the second group by 2.9%. But as a result the higher profit by 18.75 % was in the first group, which contributed to increased profitability by 2.4%. It is recommended to use up to 50% of triticale grain instead of wheat as part of complete feed for young fattening meat geese
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THE QUALITY OF THE MUSCULAR TISSUE OF YOUNG GEESE
06.00.00 Agricultural sciences
DescriptionThe article presents the materials of research on studying of influence of various levels of crude fat in the complete feed (CF) for the young geese of Lindovskaya breed on chemical composition and organoleptic quality of goose meat. In the experimental groups we increased the concentration of crude fat in the start and finish of the CF to 7.4 % and 6,85 %, respectively. As the lipid supplement, we used sunflower oil. It is established that muscle tissue of geese, consuming finishing CF with sunflower oil, has a more pronounced taste, odour, tenderness and juiciness. We may highlight the tendency to increase the proportion of monounsaturated oleic acid, in the background of the decrease of specific weight of palmitic acid in the fat muscle 60 day goslings who received the start and finish of CFs with the addition of sunflower oil
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MIXED FODDERS WITH RAPESEED OILCAKE FOR PIGS
06.00.00 Agricultural sciences
DescriptionThe efficiency of replacement of sunflower oilcake by rapeseed oilcake from double-zero rapeseed varieties was proved for mixed fodders for pigs
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06.00.00 Agricultural sciences
DescriptionStudied the investigation of the therapeutic effect of experimental mixture of four non-transducing E. coli bacteriophages and it combinations with probiotic, designed on the basis of the endemic Lactobacillus paracasei strain, previously isolated from pigs in this age of the local population in the state, the survival rate and laboratory parameters of pigs with post-weaning diarrhea. The most influence on the correction of E. coli dynamics has a combined oral administration of phage medicine and probiotic preparation established. The most influence on the dynamics of titer phages has not only the combined using (both oral and intradermal) of phage medicine with the probiotic preparation, but also an isolated ingestion of phage mixture
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CONSTRUCTION OF MUTANTS OF BACTERIOPHAGE T4 WITH REDUCED ANTIGENICITY
DescriptionA method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
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DESIGN OF THERAPEUTIC PHAGE COCKTAILS BASED ON T4-TYPE BACTERIOPHAGES: ADVANTAGES AND DISADVANTAGES
DescriptionIn the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy