name
Suprun Ivan Ivanovich
Scholastic degree
•
Academic rank
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Honorary rank
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Organization, job position
• All-Russian Research Institute of Rice
старший научный сотрудник
Research interests
Молекулярная генетика риса, ДНК-маркирование, ДНК-маркерная селекция риса на устойчивость к пирикуляриозу.
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TOP5 co-authors
Articles count: 22
Сформировать список работ, опубликованных в Научном журнале КубГАУ
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Description
The study was performed to genotype some commercial wine yeast strains using the assay of Interdelta genomic sequences. Experimental parameters of PCR to identify were optimized and optimal simplified method of DNA extraction from dried preparations of yeast cultures was define. Proven method showed a high level of resolution and can be used for the analysis of genetic diversity wine yeast in combination with SSR-markers
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VARIETY OF MICROORGANISMS GROUPS LIVING ON BERRIES OF GRAPES
DescriptionThe wide variety of microorganisms has been identified in many wine-making countries on the berries of grapes. These are yeasts of different families, forms and kinds, bacterium, mold fungi. In the article, we present the results of investigating species composition of microflora of berries of white and red types of grape, which grows in different economies of the Krasnodar region and the republic of Abkhaziya. The sowings onto the elective media were conducted for the development of entire spectrum of yeast. The grown colonies after preliminary microscoping were separated into the cultures and subjected to testing according to the culturalmorphological signs, being guided by determinants and benefits. It was established the specific variety of microflora on the surface of the berries of grapes of all investigated types, without dependence on the place of their growth. Obtained data showed that the group of yeast, which constantly is present in the complex of the epiphytic microorganisms of grapes of Saccharomyces, Pichia, Hansenula, Hanseniaspora was characteristic for all types of grapes in all investigated regions. The heterogeneity of the taxonometric composition of microflora is shown. Prevailed yeasts were of family Saccharomycetaceae, form Saccharomyces vini. A quantity of yeast of Saccharomyces vini decreases in a number of Myskhako-Caucasus-Fanagoriya, that as a whole will be coordinated with the climatic conditions. Only the type of Pinot nuar grapes had yeasts of Brettanomyces Dekkera. On the berries of Cabernets and Karaburnu we have discovered yeasts of Schisosaccharomyces acidodevoratus, causing acid-reduction. On the berries of the grapes, which grew in joint stock company APF “Fanagoriya” we haven’t revealed the presence of lactic acid bacteria Lactobacillus brevis and yeasts of the form of Schisosaccharomyces acidodevoratus. In the same farm the smallest quantity of yeastswreckers is noted, which we the forms of Pichia and Candida. In the microflora of Cabernets, Risling and, especially, Karaburnu grapes, we have found lactic acid bacteria Lactobacillus plantarum and Lactobacillus brevis. Lactobacillus plantarum. Among the bacteria the total quantity of coccic flora composes - 56 %, the rod-shaped - 44 %. Two types of active acetous bacteria are identified – Acetobacter aceti and Acetobacter xelinum. Especially high was their surface concentration on the berries of Kachich type of grapes
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APPROBATION OF MULTIPLEX SSRANALYSIS FOR DNA-FINGERPRINTS OF RICE VARIETIES
DescriptionResults of testing of multiplex sets SSR-markers for genotyping of rice varieties are presented in the article. Two sets of SSR-markers were formed: 1: RM1+ RM11+ RM70+RM122; 2: RM164+RM167+RM168. The optimal combination of DNA markers in the multiplex sets and PCR conditions allowed obtaining accurate, easily interpretable results when performing fragment analysis on automated genetic analyzer ABIprism3130. Using multiplex sets, genotyping was performed for several varieties of rice: domestic breeding and one variety – IR36 from the breeding of IRRI (Manila, Philippines). For all the studied varieties specific SSR-fingerprints were obtained. RM 168 marker showed in domestic varieties a low level of polymorphism - one allele of 97 bp. However, at the same time, the variety IR-36, showed a second type of allele 107 bp. In addition, the loci of RM1, RM11, RM167 and RM164 have unique alleles in this variety. It is consistent with significant genetic differences of these varieties and the rest of the varieties in studied sample. The proposed SSR multiplexes are promising for use in DNA certification of rice varieties and assessment of genetic diversity
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Description
Microsatellite DNA markers are currently used effectively in the study of the genetic diversity of the gene pool of fruit crops and DNA certification of varieties. For plum now there is rather limited list of works on the development of this type of DNA markers. Most often, the search for new SSR-markers for this species is carried out by checking of crossreproducibility of SSR-markers developed in other species of the genus Prunus. In this study, for the 18 SSR-markers previously developed on a peach, there was performed testing and evaluation of the prospects for the use of the genotyping of plum cultivars. Testing was made on the 4 varieties of genetically distant, belonging to the 4 different subspecies of Prunus domestica L., showed the effectiveness of their use. During the study, all tested DNA-markers were grouped together in multiplex sets comprising 3-4 markers. This allows simultaneous genotyping of 3-4 loci in a single PCR reaction. These multiplex kits are available for use in the study of genetic polymorphism of species Prunus domestica L
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Description
In the course of the work, 33 ISSR markers were evaluated for efficacy in the detection of genetic changes in regenerants of Galanthus woronowii Losinsk.. Ten markers were found suitable for genotyping according to the species under study. Five samples from the selected ten were analyzed for a sample of 20 plants of regenerants and a mother plant. The obtained data testify to genetic stability of plant material in the process of microclonal propagation
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APPROBATION OF SSR-ANALYSIS FOR DNA-IDENTIFICATION OF COMMERCIAL WINE YEAST STRAINS
DescriptionThe study was performed to genotype some commercial wine yeast strains with SSR-markers. Five polymorphic SSR-markers were tested in a selection of 15 yeast strains. Tested SSR-markers showed a high level of informativeness as well as polymorphism and can be used further to analyze the genetic diversity of wine yeast
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ДНК-ПАСПОРТИЗАЦИЯ СОВРЕМЕННЫХ РОССИЙСКИХ СОРТОВ РИСА С ПРИМЕНЕНИЕМ SSR-МАРКЕРОВ
DescriptionIn the presented study, we have performed genotyping of modern Russian rice cultivars using microsatellite DNA-markers. The markers showed different level of allelic polymorphism: from 2 to 8 alleles per locus. For all studied cultivars,unique DNA-fingerprints were obtained
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A MODIFIED PROTOCOL OF RNA ISOLATION FROM MATURE LEAVES OF GRAPES FOR RT-PCR
DescriptionIsolation of high-quality RNA from the tissues of perennial woody plants, including woody grape vines, is very difficult due to the high content of phenolic compounds, secondary metabolites and polysaccharides and the ribonuclease activity of destroyed tissues. Most of the existing methods require either large time or financial costs, or do not give reproducible results in the case of RNA extraction from mature grape tissues. The modified isolation protocol is based on a combination and modification of the known RNA extraction methods, taking into account the characteristics of mature grape tissues. Existing commercial kits for the isolation of RNA from plant tissues showed a low efficiency of RNA extraction from mature grape tissues, primarily associated with "varietal specificity". Reproducible results in the extraction of RNA showed CTAB-method, however, it has several significant drawbacks associated with the duration of the extraction and the complexity of the processing of an RNA preparation with a DNAase. The developed method is based on increasing the concentration of mercaptoethanol and polyvinylpyrrolidone in the extraction buffer, eliminating the stage of RNA selective precipitation via LiCl, and replacing it with deposition on a silica-based membrane (SiO2) followed by processing with DNA-ase. and increase the purity of the preparation of RNA from genomic DNA in comparison with the original method. A modified isolation protocol was developed based on a combination and modification of known RNA extraction methods, taking into account the characteristics of mature grape tissues. This solution allows to obtain reproducible quantity and quality of RNA for the subsequent synthesis of cDNA and RT-PCR
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Description
Professional apple gardening is bound to particular risks, of which is essential losses of a harvest because of diseases. An apple scab, the caused Venturia inaequalis (Cooke) G. Winter, brings the greatest loss. The main approach in monitoring of a scab of an apple-tree is creation of grades, steady against pathogen. In the present work we have carried out phytopathology testing of generations of the seedlings received from the free pollination of six forms of a crab of Malus orientalis from a collection MOS VIR (Maykop) characterized by a relative resistance to the apple scab in 2-3 classes of damage by long-term data. For infection we used inoculum, consisting both of natural population of a scab, and of strains of pathogen of various cultivars and geographical origin. When carrying out padding infection increase in force of an infectious background that can be bound to selection of the plants of biotypes of a fungus that are most adapted for genotypes was noted. It is recommended for precise elimination of unstable plants at selection at early stages of an ontogenesis to carry out more than one serial infection during the season. By results of the phytopathological testing, we highlighted three Malus orientalis forms from the six studied No. 17982, 17985 and 3080 the most perspective stability genes for an introgressiya in a cultural gene pool of an apple tree
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Description
Industrial horticulture assumes the most effective use of the potential of varieties. One of the key factors determining the yield of garden plantings is the effectiveness of pollination. To obtain the maximum yield, it is necessary to ensure maximum pollination during the flowering period. For this reason, much attention has been paid to the selection of pollinators. Crab-apple forms are promising for use as pollinators, so this work was aimed at identifying the most common alleles of the self-incompatibility gene in the crab-forms using the molecular genetic method of analysis. The object of the study was 29 apple-tree creams and 3 elite selection forms. They carried out the molecular genetic identification of alleles S2 and S10, which are among the most common apple trees in the world gene pool. Allele S2 was identified in 16 samples (14 forms and 2 elite selection forms), while S10 allele in one sample (elite form 12/2-20 (24-28)). Data on the allelic composition of the S gene in the samples studied are of value for the formation of a genetic passport on the compatibility of the studied samples of apple with modern industrial varieties