Krasnodar Scientific Research Institute of Veterinary
Author list of organization
List of articles written by the authors of the organization
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MICROBIAL ASSOCIATIONS OF BIOHUMUS AND HUMIC SUBSTANCES DERIVED FROM LIVESTOCK WASTE
06.01.01 General agriculture, crop production (agricultural sciences)
DescriptionThe article presents the results of the study of microbiological composition of biohumus and humic substances obtained by fermentation method according to the technology developed by the authors. The problem of processing livestock wastes is caused on the one hand by the occurrence of large volumes of them, and on the other hand by the addition of such important elements as nitrogen, phosphorus, potassium, etc., which can be used as bioelements. The goal of research was to develop a technology for the production of humic compounds from biohumus and to study the microbial communities that occur in them. Results of researches showed that for biohumus with high content of humic substances, bovine manure must be composted with chopped straw at the ratio of 8:1. Biocomposting of manure and crushed straw using the developed technology enables to obtain biohumus containing humic substances of 20 g/l. Among the physiological groups of biohumus microorganisms, the most numerous groups are aminoautotrophies and ammonifiers - 231.6×106 and 130.33×106 CFU/g, respectively. At the same time the grouping of cellulose-decomposer actinomycetes in biohumus was quite numerous and amounted to 0.93×103 CFU/g.
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06.02.00 Veterinary and Husbandry
DescriptionProviding the population with basic food products, sources of proteins, fats, carbohydrates, vitamins and other essential nutrients is one of the main issues of ensuring the security of the country, while the requirement for food safety is paramount. Production of feed additives (concentrates), which improve feed conversion, while being natural and safe for animal health, acquires the greatest importance in the conditions of intensification of agriculture and increasing the competitiveness of livestock farms. Probiotic microflora, which, in competition with pathogenic, displaces the latter from the gastrointestinal tract, can be considered as one of the effective methods of controlling undesirable microflora in the digestive tract. In world science, research is actively conducted in the direction of searching for effective probiotic cultures. A promising way to increase the effectiveness of the use of probiotic feed additives is their addition to prebiotics, for example, including vitamins (antioxidants) and mineral substances (selenium). The purpose of this work was to determine the optimal ratio of biologically active substances contained in the feed vitamin-mineral concentrate, and probiotic microflora contained in the feed probiotic additive Bacell-M, for the development of complex feed concentrate. Using the Harrington's desirability function, the optimum ratio of biologically active substances and probiotic microflora in the recipe of the complex feed concentrate is determined, ensuring the maximum reduction in the serum levels of alanine aminotransferase in the blood serum of experimental animals, which characterizes the normalization of barrier organs
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06.02.00 Veterinary and Husbandry
DescriptionProviding the population with basic foodstuffs, sources of proteins, fats, carbohydrates, vitamins and other essential nutrients is one of the main issues of ensuring the security of the country, while the requirement for food safety is paramount. Production of feed additives (concentrates), which improve feed conversion, while being natural and safe for animal health, acquires the greatest importance in the conditions of intensification of agriculture and increasing the competitiveness of livestock farms. Probiotic microflora, which, in competition with pathogenic, displaces the latter from the gastrointestinal tract, can be considered as one of the effective methods of controlling undesirable microflora in the digestive tract. In world science, research is actively conducted in the direction of searching for effective probiotic cultures. A promising way to increase the effectiveness of the use of probiotic feed additives is their addition to prebiotics, for example, including vitamins (antioxidants) and mineral substances (selenium). The purpose of this work was to determine the optimal ratio of biologically active substances contained in the feed vitamin-mineral concentrate, and probiotic microflora contained in the feed probiotic additive Bacell-M, for the development of complex feed concentrate. Using the Harrington's desirability function, the optimum ratio of biologically active substances and probiotic microflora in the recipe of the complex feed concentrate is determined, ensuring the maximum reduction in the serum levels of alanine aminotransferase in the blood serum of experimental animals, which characterizes the normalization of barrier organs
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ANALYSIS OF MANIFESTATIONS OF UROLITHIASIS IN CATS
06.02.00 Veterinary and Husbandry
DescriptionIn this work, the authors presented the results of monitoring urolithiasis in cats in Krasnodar in the period from 2016-2017. The authors established the sexual, age and breed predisposition of animals to this pathology; the seasonality of the disease was studied. An analysis of the actual data and reports of various authors made it possible to conclude that urolithiasis in cats is a polyethological disease. As a result of carried out hematological studies, the presence of an inflammatory process, which was manifested by lymphocytopenia, an insignificant increase in neutrophils and monocytes, as well as hematocrit, was ascertained. A laboratory analysis of urine showed that urine, as a rule, is turbid with an admixture of blood and sand, precipitating rapidly. The authors found an increase in the volume of urine in sick animals due to the inability to remove it, the specific gravity fluctuated within the limits of 1.015-1.020; pH = 7.5-8. With microscopy of the urine sediment, both unorganized and organized sediments were detected. The ultrasound examination of the bladder showed the presence in its cavity echo-positive particles in a suspended state, as well as sections of the desquamated mucosa
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FEED CONCENTRATES: TRADITIONS AND PURPOSES OF THEIR APPLICATION IN POULTRY
06.00.00 Agricultural sciences
DescriptionThe article shows the historically developed situation and the actual state of the production practice in chronic mycotoxicosis of a bird. The main feed problems in poultry farming are shown through the prism of a review of market proposals for feed concentrates. An overview of preventive and compensating agents on the market, as well as a brief description of feed concentrates based on beta-carotene for fighting mycotoxicosis. It is shown that the vitamin-mineral fodder concentrate "Tetra +" is an alternative to the existing feed concentrates on the market, the effectiveness of which has been confirmed in experiments on laboratory animals and in poultry feeding. Based on the analysis, a conclusion was made about the relevance of studies to identify the effectiveness of vitamin-mineral concentrate "Tetra +" in livestock
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Description
The use of intensive milk production technologies in practice, nonobservance of the principles of feeding and keeping animals leads to a decrease in the level of compensatory-regenerative processes in the body, violation of general and specific metabolic reactions, emergence of a number of pathological conditions, and, primarily, hepatopathies leading to a decrease in the productivity of dairy cattle, to the birth of unviable young animals and to the impossibility of their further exploitation. Therefore, on the background of early diagnosis of violations of the liver it is necessary to conduct adequate pharmacoprophylaxis, which prevents further development of hepatopathies with the help of hepatoprotectors. These preparations include a new injectable one – livazen, the mechanism of action of which works due to its antioxidant and membrane-stabilizing action. The preparation has a lipotropic effect, favorably affects the antitoxic and pigmentary function of liver, and increases resistance to hypoxia and various kinds of intoxication. Research conducted to study its effect in the system of prophylactic measures of hepatosis in sterile cows showed that on the background of the use of lavazen there was a significant increase in total protein by 16,6% and increase in albumins - by 13,8%. The level of γ-globulins, on the contrary, decreased by 20,9%. The study of the preparation had a normalizing effect on lipid metabolism, which was manifested by an increase in the concentration of triglycerides in 3,1 times and increase in cholesterol by 24,7%. An increase in the concentration of calcium by 14,5% was also found out. The use of livazen allowed to ensure a tendency towards a decrease in enzyme activity (according to AST - by 6,9%, by ALT - by 24,4%, according to alkaline phosphatase - by 11,6%), in contrast to control animals whose aspartate aminotransferase increased by 11,3% from the initial; ultrasonography of liver of cows confirmed the expressed effectiveness of the pharmacoprophylaxis
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Description
In this study, the authors have defined the acute, subchronic and chronic toxicity of Uritosom preparation, and also its skin-irritating and sensitizing action. The studies that determined the acute toxicity of the drug Uritosom found that remedy which intended for the treatment and prevention of diseases of the genitourinary system with internal method of appointment during the entire observation period did not cause the death and acute toxicity in laboratory animals. In the study of subchronic and chronic toxicity of the drug Uritosom throughout the observation period in both experimental groups death and acute intoxication in animals not registered, also there were no negative phenomena in behavior, reflexes were saved. Consequently, the preparation according to the degree of impact on the body of warm-blooded animals refers to low-hazard substances (4th class of hazard according to GOST 12.1.007-76). Study of the irritant action of Uritosom preparation was carried out in two series of experiments. In the first series of experiments was determined irritating action by conjunctival swab sampling method, in the second - by skin application. The study of the sensitizing action of the drug was determined by the method of maximum sensitizing effects. The authors have found that the preparation has no irritating and sensitizing effect on the tissue in its application zone. On this basis, Uritosom preparation at different ways of application is low-toxic and it can be recommended for clinical research
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USE OF FLUCONAZOLE FOR TREATMENT OF CHRONIC ENDOMETRITIS AT COWS
DescriptionThat article is of interest to practicing veterinarians. Currently, the biggest obstacle in the development of dairy farming is infertility at cows, which is caused by the presence of diseases of the reproductive organs, most of which is occupied by acute and chronic endometritis. The frequency of occurrence of chronic endometritis at cows reaches high levels, from 10 to 66.3%, averaging 14%. One of the direct causes of chronic endometritis is association of pathogenic and opportunistic microorganisms and fungi. Many chemotherapeutic agents which are designed for prophylaxis and treatment of acute postpartum endometritis do not give a high therapeutic effect in the treatment of chronic endometritis or amount and frequency of application drugs is very high. Analyzing the data, the authors developed a new drug which has fungicidal and antibacterial actions - Florinazol. The experiments showed that the use the Florinazol preparation in combination with myotropic, pathogenetic and vitamin preparations gives 90% of therapeutic efficiency, while the frequency of administration of the drug was on average 3 fold, and the duration of treatment was 5 days
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TREATMENT OF CHRONIC ENDOMETRITIS WITH THE NEW DRUG FLORINAZOL
DescriptionIn the article data on spread of chronic endometritises at cows are provided. The microflora range, being in genitals of patients with a chronic endometritis of cows is determined. We have developed a new drug called Florinazol and we also determined his antimicrobic and fungicide activity. Researches on determination of acute and chronic toxicity, and also irritant action on a mucous membrane of a uterus and a vagina are conducted. Researches on studying of influence of Florinazol on blood biochemistry at rabbits are conducted. The optimum therapeutic dose and frequency rate of its introduction is determined. By results of researches it is established that 24,8% of the cows containing on a farm with loose housing content and 10,6% of cows on a farm with fastened content have a chronic endometritis. At the same time in uterus content in 84,3% of cases isolated associations of bacteria and mushrooms in 65,2%, in 34,8% - monocultures of microorganisms. Offered by us means the danger class belongs to substances lowdangerous by the 4th. Doesn't possess irritant action, doesn't exert a negative impact on blood biochemistry, and also has outstanding therapeutic performance of 90% in case of its application in a dose of 100 ml intra-vaginal at an interval of 48 hours
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EFFICIENCY OF TETRA+ FEED ADDITIVE APPLICATION IN CHRONIC MYCOTOXICOSES OF PIGS
06.00.00 Agricultural sciences
DescriptionThe article describes the efficiency of a feed additive called "Tetra+" during chronic mycotoxicoses of pigs. The purpose of carrying out series of experiments on sows was to determine the therapeutic and prophylactic effectiveness of innovative feed additive "Tetra+" during combined chronic mycotoxicosis in pigs and to study the influence of feed additive on the growth, weight, preservation and overall resistance of sows. The results of the experiments revealed that a feed additive "Tetra+" has had a positive impact on large-fruited of pigs; it can also be concluded that the feed additive "Tetra+" has a positive effect on the metabolism of proteins, carbohydrates and has antitoxic properties