North Caucasian federal scientific center of horticulture, viticulture, winemaking
Author list of organization
List of articles written by the authors of the organization
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A MODIFIED PROTOCOL OF RNA ISOLATION FROM MATURE LEAVES OF GRAPES FOR RT-PCR
DescriptionIsolation of high-quality RNA from the tissues of perennial woody plants, including woody grape vines, is very difficult due to the high content of phenolic compounds, secondary metabolites and polysaccharides and the ribonuclease activity of destroyed tissues. Most of the existing methods require either large time or financial costs, or do not give reproducible results in the case of RNA extraction from mature grape tissues. The modified isolation protocol is based on a combination and modification of the known RNA extraction methods, taking into account the characteristics of mature grape tissues. Existing commercial kits for the isolation of RNA from plant tissues showed a low efficiency of RNA extraction from mature grape tissues, primarily associated with "varietal specificity". Reproducible results in the extraction of RNA showed CTAB-method, however, it has several significant drawbacks associated with the duration of the extraction and the complexity of the processing of an RNA preparation with a DNAase. The developed method is based on increasing the concentration of mercaptoethanol and polyvinylpyrrolidone in the extraction buffer, eliminating the stage of RNA selective precipitation via LiCl, and replacing it with deposition on a silica-based membrane (SiO2) followed by processing with DNA-ase. and increase the purity of the preparation of RNA from genomic DNA in comparison with the original method. A modified isolation protocol was developed based on a combination and modification of known RNA extraction methods, taking into account the characteristics of mature grape tissues. This solution allows to obtain reproducible quantity and quality of RNA for the subsequent synthesis of cDNA and RT-PCR
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PHENOTYPING OF HIGH-QUALITY TECHNICAL GRAPES OF AZOSVIV SELECTION IN THE AMPELOGRAPHIC COLLECTION
06.00.00 Agricultural sciences
DescriptionA variety determines the direction of use of grape products and plays a leading role in improving its quality. Preservation of genetic resources of grapes and their studying are very important for modern science. The studied material makes it possible to use the selected genetic characteristics for breeding new varieties of grapes, taking into account the requirements, and introducing them into production, as well as highlighting the most valuable varieties for transferring them to the state variety testing. Among a wide variety of grape varieties, in the ampelographic collection there are varieties of AZOSVIV selection of the technical direction: Krasnostop AZOS and Dostoiniy, for which complete phenotyping was carried out. The phenological and agrobiological information on the grape varieties studied for 2015- 2017 is collected. The presented grades of grapes of the technical direction are characterized by high quality of production. The wines from the varieties Krasnostop AZOS and Dostoiniy are competitive, characterized with high consumer properties; they enjoy the well-deserved fame and were nominees of exhibitions and holders of diplomas repeatedly. Based on the results of microsatellite profiling, the varieties showed a sort-specific general combination of alleles. At the same time, in each locus there is one identical allele, which corresponds to the fact that the varieties Krasnostop AZOS and Dostoiniy have one common parent (Phylloxer-resistant Gemete). The received formulas of a variety (DNA-passports) in practice can be effectively applied to determine the cleanliness of planting stock and plantations of grapes, specification of the parent forms of the sample and in the disputed questions of the authorship of the variety. The study was supported by Federal Agency for Scientific Organizations program for support the bioresource collections
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Description
The pathogenic agent of apple scab disease, Venturia inaequalis (Cooke) Winter, causes significant damage to the industrial production of apples. Studying the pathogen in pure culture is important for solving the theoretical and practical issues of its biology. The sporulation ability of the Venturia inaequalis laboratory culture is an important diagnostic feature in morphological culture analysis, in the test for sensitivity to fungicides, when creating a collection inoculum for an artificial infection. We have studied the morphological features of conidiogenesis of pure V. inaequalis culture, such as the location of conidiogenic structures and their form. For the first time in the laboratory culture of the pathogen, sporulation in the thickness of agar on the substrate mycelium was demonstrated. Conidiogenesis proceeded according to the blastic-annellidic type. Depending on the location of the conidiogenic structures on the aerial or substrate mycelium, their morphology was different. Conidiogenic areas in the substrate mycelium could be observed with the unaided eye, in the form of hyphal grit, while they were conidia conglomerations in each annelid. Annellide had a curved shape. On the aerial mycelium, annelids were straight and always had only one conidium. Differences in the morphology of conidiogenic structures are supposed to be related to the physical conditions of the environment in which sporulation takes place. In the agar, each mature conidium remaining at the apex of the annelid interferes with the formation of the next one, which results in its bending. The fixed arrangement of conidiogenic structures and forming conidia in the thickness of agar allows the use of substrate sporification for model studies of the conidiogenesis process
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GRAPE VARIETIES OF THE ANAPA AMPELOGRAPHIC COLLECTION AND THEIR USE IN BREEDING WORK
06.00.00 Agricultural sciences
DescriptionIn the world, huge work is being done to create global information banks of plant genetic resources. The need for conservation and rational use of genetic resources in modern conditions has become very relevant. Genetic collections are the basis for selection work: creating new varieties, replenishing the assortment with new, classic, introduced and native varieties, adapted to the natural (soil and climatic) conditions of the cultivation sites. In the Anapa ampelographic collection, 4911 varieties of various ecogeographical and genetic origin are represented, the use of which provides great opportunities for creating and breeding new varieties, their state testing and zoning. In the gene pool of the ampelographic collection, most varieties of V. vinifera L. grapes are represented by local honeycombs of different regions of the grape culture, and more than one fourth of varieties of V. vinifera L. are obtained from intraspecific crossings. Despite the value of local varieties of grapes, they do not always meet all the requirements of production. Therefore, to improve the local assortment, it is necessary to carry out selection - breeding new varieties of grapes. This article presents the long-term results of the work on the selection of varieties of table and technical directions in the Anapa ampelographic collection used for breeding. Also, the role of the genetic collection in the creation of new generation varieties by combinative selection is shown - as donors for creating new varieties of grapes. Dedicated varieties are the sources of valuable traits, which are given primary importance and are especially important for the viticulture of the Russian Federation