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06.00.00 Agricultural sciences
DescriptionTwo methods were developed, one to assess experimental results by reducing a number of developmental parameters to the overall quality, leading to the overall quality criterion, and the other to optimize processes affected by numerous interacting factors, in-vitro plant development in this case, by applying a mathematical design of experiment. Single-bud cuttings with one leaf of two Vitis vinifera L. genotypes were excised from the central part of two-month-old in-vitro grown plants and used as explants. The explants were established on bridges of filtering paper in liquid media and on solid media. Eighteen modifications of these media contained five macro-elements, each at three concentrations, and the distribution of these macro-element concentrations followed the law of random numbers (Experiment I). Parameters characterizing arm, leaf and root development of two-month-old plants of each study genotype established on each liquid or solid medium were reduced to the overall quality of plant development. Since the study genotypes differed in the ability to grow on liquid and solid media with different macro-element concentrations and also in the ability to utilize these macro-elements, the dependence of in-vitro plant development on macro-element concentrations was described by different regression equations. That is why the regression equation describing the average outcome of plant development in the two genotypes on liquid and solid media was not as significant and the description of the process was not as adequate (determined) as regression equations calculated for each process. The regression equation which describes the dependence of the average outcome of plant development on macro-element concentrations in Experiment I is as follows: y5 = 0.027 + 0.116x22 + 0.109x2x4 + 0.106x2x3 + 0.114x4. Stepwise calculation of macro-element concentrations to optimize in-vitro plant development was done based on macro-element concentrations of the initial medium of Experiment I as starting points since that medium was best efficient for this purpose by using regression equation (y5) and algorithms of multiple curvilinear stepwise regression according to the Box-Wilson method of steepest ascent. Experiment II was undertaken where macro-element concentrations (‘steps’) were calculated in a stepwise manner to optimize in-vitro plant development of the two V. vinifera genotypes and the rootstock ‘Kober 5BB’. This led to a liquid medium and a solid one which enabled a better plant development in the three genotypes relative to the use of controls: media with ½ MS macro-elements and the initial medium whose macro-element concentrations entered as starting points to calculate ‘steps’ for optimization of in-vitro plant development. The optimized medium contained macro-elements: 318 mg l-1 NH4NO3 (x1), 1188 mg l-1 KNO3 (x2), 370 mg l-1 MgSO4 7H20 (x3) (MS), 370 mg l-1 KH2PO4 (x4), 331 mg l-1 CaCl2 (x5) (MS), and other substances at optimum concentrations adjusted earlier: ¼ MS Fe-EDTA, ¼ MS micro-elements, 20 mg l-1 myo-inositol, 0.1 mg l-1 thiamine (MS), 0.5 mg l-1 nicotinic acid (MS), 0.2 mg l-1 pyridoxine, 2 mg l-1 glycine (MS), 0.1 mg l-1 indole-3-acetic acid, 10 g l-1 sucrose and, only for solid media, 7 g l-1 Difco agar. The optimized medium may be used for propagation of virus-free plants, valuable clones and grapevine genotypes created by gene engineering. The mathematical design of experiment reported in this paper which enables stepwise optimization of in-vitro plant development may be used both in agriculture and in the food industry
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INNOVATIONS OF VINE GROWING IN RUSSIA. 20. TRIMMING OF VINE BUSH
06.00.00 Agricultural sciences
DescriptionIn the article the short description of theoretical bases of vine bushes trimming, ways of trimming, methods of setting the optimum loading of a bushes when trimming, optimum terms of trimming young and fructifying plantings, key rules and techniques of trimming of bushes are specified
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INNOVATIONS OF VINE GROWING IN RUSSIA. 18. ESTABLISHMENT OF VINEYARD
06.00.00 Agricultural sciences
DescriptionIn this article the questions of rational placing of bushes on sites when establishing new vineyards are taken up; requirements to a planting material - to cuttings and transplants imparted and own-rooted, one-year and vegetative, technologies of their preparation for planting and planting by different ways, etc. are concerned
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INNOVATIONS OF WINE GROWING IN RUSSIA. 17. THE ORGANIZATION OF ESTABLISHMENT OF VINEYARD
06.00.00 Agricultural sciences
DescriptionIn the given section questions of a correct choice of the ground area for establishment of a vineyard at new place are examined, considering high insistence of the sample to heat and light; the question of a choice of soil is connected with reception of a high crop of high quality grapes and products of its processing. Innovational technologies of soil preparation and the territory organization for a vineyard establishment are described, main principles of selection of table and technical grades, their placing on a site are given
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INNOVATIONS OF WINE GROWING IN RUSSIA. 16. MASS-PRODUCTION VARIETIES OF GRAPES
06.00.00 Agricultural sciences
DescriptionIn this article color photos of 66 mass-production grades which have been removed from division into districts and/or excluded from the State registry of grapes of the Russian Federation as conceded to more competitive on adaptability and profitability, and also of phenotypes removed from the State test are presented. The number of mass-production grades has reached 460 for today. Although the term of "mass-production" grades is not very good, but in wine growing it has appeared to be comprehensible enough and clear to experts for a long time
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INNOVATIONS OF WINE GROWING IN RUSSIA. 15. SEEDLESS GRADES OF GRAPES
06.00.00 Agricultural sciences
DescriptionIn the article the information of a problem of seedless grape berries and a gene fund of seedless grades, the best 44 genotypes from which are presented in color photos is resulted. In industry of the Russian Federation they are cultivated on the area of about 120 hectares. These grades of grapes possess useful signs and properties, are valuable gene-sourсes and donors of polygenes for selection. Among the resulted grades of grapes in Russian zoned assortment of seedless grapes there are only 2 for today. Questions of this direction of researches are closely stated in our manual «Seedless grades of grapes» (Krasnodar: KubSAU, 2008. - 160 p., 58 pics)
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INNOVATIONS OF WINE GROWING IN RUSSIA. 14. THE NATIVE PERSPECTIVE VARIETIES OF GRAPES
06.00.00 Agricultural sciences
DescriptionIn this article 59 color photos and short characteristics of perspective native domestic varieties of grapes from 104 growing in National Ampelographical Collection of Russia (Anapsky area of Krasnodar region) are presented. These grades are national property of our state. Our aim is to find, collect and save 152 grades described in official ampelographical editions, but not shown in NACR
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INNOVATIONS OF WINE GROWING IN RUSSIA. 13. THE NATIVE ZONED VARIETIES OF GRAPES
06.00.00 Agricultural sciences
DescriptionIn the article ampelographical descriptions and color photos of all of 13 native grades of the grapes zoned at 6 North Caucasian region of Russian Federation are presented. These varieties are national property of our state and pride of domestic wine growing
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INNOVATIONS OF WINE GROWING IN RUSSIA. 12. CHARACTERISTICS OF TEMPORARILY PERMITTED GRADES OF GRAPES
06.00.00 Agricultural sciences
DescriptionIn article the characteristics of 111 temporarily permitted grades of grapes, having outstanding ampelographical indicators, basically, for their effective use in amateur wine growing in Russia are resulted. Color images in the form of photos of these grades of grapes are presented in earlier published section 9 of the given collective monograph
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INNOVATIONS OF WINE GROWING IN RUSSIA. 11. THE CHARACTERISTICS OF PERSPECTIVE SORTS OF GRAPES
06.00.00 Agricultural sciences
DescriptionIn the article the characteristics of 64 perspective grades of grapes which are valuable for production use in southern regions of Russia are resulted. Color images in the form of photos of these grades of grapes are presented in earlier published section 8 of the given collective monograph