06.02.02 Veterinary Microbiology, Virology, epizootology, Mycology with mycotoxicology and immunology (veterinary sciences)
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Description
We took 20 samples of centralized water for bacteriological studies at two farms of the collective farm “Limann” and the collective farm “50 years of the name of the October Revolution” of the Neklinovsky District of the Russian Federation and sent to the Rostov Regional Veterinary Laboratory. Thus, according to the results of laboratory studies of water, it was found that in the first farm in 7 samples out of 10 under No. 1, 2, 4, 5, 8, 9, 10, common coliform bacteria were found (the number of bacteria in 100 ml according to MUK 4.2. 1018-01), as well as thermo-tolerant coliform bacteria (the number of bacteria per 100 ml according to MUK 4.2.1018-01), in the second farm in 5 samples out of 10 under No. 3, 4, 5, 7, 8 common coliform bacteria (number of bacteria per 100 ml according to MUK 4.2.1018-01), as well as thermo-tolerant coliform bacteria (number of bacteria per 100 ml according to MUK 4.2.1018-01). According to the results of studies using the rapid test "Biocontrol" revealed that in the first farm in 2 samples out of 10 under No. 4.9 per 1 ml of bacteria 105, which indicates water pollution, and in 5 out of 10 samples No. 1, 2, 5, 8, 10 per 1 ml of bacteria 107, which indicates a strong pollution of water and is the basis for the prohibition of watering animals. In the second household, in 3 samples out of 10 under No. 4, 5, 8 per 1 ml of bacteria 105, which indicates water pollution, and in 2 out of 10 samples No. 3, 7 per 1 ml of bacteria 107, which indicates severe water pollution which is not recommended for animals. Thus, the studied water samples by the bacteriological method coincide with the “Biocontrol” rapid tests, which makes it possible to practically use it in farms
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Description
The aim of the following research is the studying of the efficacy in probiotic drug Probiolact (0.5% and 1.0%) usage, being in combination with Milkshake supplement. The given complex mixture is meant for medicative-phylactic purposes focused on the reduction of young ones death rate owing to gastro – intestinal diseases when neo-natal period. It is specified that the above-mentioned mixture affects the livestock indices. Thus, the average growth of baseline for the period of 30 days experimenting has been 7.4 kg, whereas in 2 tested groups it accounted for 10.3 kg and 11.6 kg, consequently. Therefore, the best possible daily phylactic doze of Probiolact (1%) and Milkshake mixture demonstrates the positive effect on the pro-cess of bifido- and lactoflora formation in calves’ intestinal tract due to normal flora cells quantity increase. With that, intestinal tract contamination level of disease-producing flora gets lower, and the natural resistance of cell-mediated immunity grows. Thus, two major problems of factory farming (i.e. the reduction of young death rate and surplus guarantee) can be solved with giving calves the mixture to drink, along with the first portions of colostrum, and during the following 30 days
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OBTAINING DIAGNOSTIC KITS FOR SERODIAGNOSIS OF CARNIVOR INFECTIOUS HEPATITIS
Description
The article is devoted to the current problem of differential diagnosis of diseases of viral etiology in small pets. Viral diseases of dogs and cats are widely distributed among both pedigree and non-pedigree animals. The spread of diseases is facilitated by the increase in the number of small pets, the popularization of pet maintenance, cross-border operations associated with the movement of animals. The importance of express methods in laboratory diagnostics of infectious diseases is considered. Reducing the time of diagnosis contributes to the effectiveness of therapeutic and anti-epizootic measures. The place of infectious hepatitis of carnivores in the structure of morbidity of dogs is analyzed. The article describes the research in determining the optimal systems for the cultivation and subsequent isolation of the infectious canine hepatitis virus in high titers. If viral biomass needs to be increased, the choice of a cultivation system for maximum harvest is of paramount importance. The authors propose a method of obtaining and purification of viral antigens to use them for preparation of diagnostic kits. The release of the isolated fraction of ballast substances, which provides rapid response and strong binding to specific immunoglobulins is essential for the preparation of antigenic drugs. It is important to obtain antigens with a high degree of purification to get reliable results in the formulation of serological reactions
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PRÉVALENCE ET IMPACT ÉCONOMIQUE DES STRONGLES GASTRO-INTESTINAUX DES CAPRINS AU NORD DU BURUNDI
Description
Une enquête et une étude parasitaire des caprins ont été réalisées dans les trois provinces du Nord du Burundi appartenant aux régions naturelles de Bweru, Bugesera, Buyenzi et Mugamba. L’étude montre que le système extensif combinant le gardiennage et l’attachement au piquet vient en première positon (44%) par rapport au pâturage au piquet (28%). Les résultats montrent que 75,84% des éleveurs font l’élevage des caprins spécialement pour les vendre, 22,15 % pour la production du fumier et 2,01% pour l’autoconsommation. L’enquête sur 424 chèvres pendant la saison pluvieuse et sur 538 chèvres pendant la saison sèche effectuée sur 150 ménages montre une dominance des caprins de race locale (78,4%) par rapport aux races exotiques et croisées (21,6%). L’analyse coprologique a montré la présence des strongles gastro-intestinaux de genres Ostertagia, Chabertia, Haemoncus, Paramphistomum, Nematodirus, Bunostomum, Dictyocaulus, Oesophagostomum, Trichostrongulus, Cooperia, Diclocoelus et le genre Toxocola. Les genres Ostertagia et Chabertia étant dominants avec 20.10% et 18.76% respectivement. Les jeunes chèvres de moins d’une année étant significativement plus infestées par les strongles gastro-intestinaux que les chèvres adultes (p< 0.05). Ce parasitisme gastro-intestinal affecte négativement l’économie des éleveurs par des dépenses liées aux traitements. Il se traduit aussi par une réduction de leur croissance voire une mortalité lors d’infestation forte et influence négativement sur le gain du poids de l’animale et sa croissance en générale (p=0.001)
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Description
We have performed a comparative estimation of three coccidiostats when experimental eimeriosis of chickens, infected with a coccidia field isolate from poultry production sites. Chickens performance treated with robenza, chicocin and monenza was almost the same. The anti-coccidiosis index was also the same in all three groups, not exceeding 160, which indicates the low sensitivity of coccidiosis agent to all studied drugs. An ionophore antibiotic (chicocin), a synthetic anti-coccidia drug (robenz) and a complex (monenza) consisting of an ionophore antibiotic and a synthetic anti-coccidia drug can be used in this poultry complex for the prevention of coccidiosis for a short time. But there is a need for constant monitoring and selection of new combinations, as partial resistance of coccidia to drugs can be transmitted genetically and will only increase over time, making coccidiostats ineffective
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EXPERIENCE OF USING "HYPONAT BPO" DISINFECTANT FOR REFRIGERATOR CHAMBERS DISINFECTION
Description
The article presents the results of production tests of previously developed refrigeration disinfection regimen “Hyponat BPO”. As a result of production tests, it was found that the disinfectant “Hyponat BPO” provides 100% disinfection of refrigerated chambers, as well as auxiliary equipment at meat processing plants. Thus, it was determined that the positive effect of surface disinfection during control according to the E. coli test culture was achieved by using a 2% solution at an exposure of 30 minutes, staphylococcus with a 3% solution and exposure of 50 minutes, and with a VLM control, a 4% solution and exposure of 60 minutes
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Description
Protection of farm animals from diseases remains one of the priority tasks for veterinary practice. A healthy animal is the key to obtaining high sanitary quality of livestock products. Among the veterinary-sanitary and organizational-economic measures carried out for the prevention and control of infectious diseases, disinfection is of particular importance. In the laboratory of veterinary and sanitary expertise of VNIIVSGE-branch of the Federal State Budget Scientific Institution Federal Research Center of the Russian Academy of Medical Sciences, a new composite preparation “Hyponatum BPO” has been developed and is being tested. The studies found that the "Hyponat BPO" has a high disinfecting effect against gram-positive and gram-negative vegetative microflora, located on surfaces of various materials (wood, concrete, tile, stainless steel, plastic), both with the presence of protein protection, and without it
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RATIONAL PHARMACOTHERAPY FOR PANCREATITIS IN DOGS
Description
Diseases of the digestive system are one of the huge problems of veterinary medicine and pharmacy. This group of diseases not only affects all age groups of animals, but can occur even in newborn puppies. It is very difficult to talk about the breed predisposition to pancreatitis in animals. Pancreatitis occupies one of the leading places in the structure of digestive diseases in animals. The urgency of this veterinary problem is increasing every year. Acute pancreatitis manifests itself suddenly, most often against the background of gross violations in feeding, with the disease of the duodenum and gallbladder. In this case, there is a violation of the outflow of secretion into the ducts of the pancreas. In the tissues of the pancreas there are diffuse changes up to necrosis. Severe inflammation of the pancreas leads to a sharp deterioration in the general condition of the body, sharply reduced blood pressure. The animal is in great pain, vomiting is observed. The animal needs urgent care. The disease can be chronic. Periods of exacerbation are repeated from time to time. The owners usually do not call veterinary specialists. Since the disease is manifested by symptoms characteristic of many pathologies of the gastrointestinal tract, it is therefore difficult to diagnose. In the history of the animal there are both diarrhea and constipation, the animal has a reduced appetite, in hotel cases there is cachexia. In hematological studies, we can note an increase in sugar levels. Therefore, for the differential diagnosis of the animal, we carry out ultrasound, x-ray diagnostics all together with biochemical methods of blood tests. In the future, when choosing treatment tactics, all diseases that could accompany inflammation of the pancreas are taken into account
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CORRECTION OF DYSBIOTIC DISORDERS IN BEES BY USING PROBIOTIC MEDICATIONS
Description
This article provides an overview of the need of the use of probiotic supplements and medications in the diets of insects, in particular bees and their families. We present a list of both Russian and foreign probiotics and their complexes, which are used in beekeeping for the treatment and prevention of various diseases, are shown. Their effectiveness is shown not only for the prevention and treatment of microbial disorders in the gastrointestinal tract of bees due to the manifestation of antagonistic properties in relation to opportunistic and pathogenic microflora, but also the possibility of their use as tools that can increase the immune status, the safety of bee families, and life expectancy. The article shows data on the positive effect of probiotics on the physiological and biochemical parameters of honeybees, activation of metabolic processes in the formation of a fat body, growth of strength and weight of larvae, development of pharyngeal glands, etc.
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Description
The article demonstrates the results of therapeutic and preventive effectiveness of the use of lactic acid bacteria – Lactobacillus brevis and Lactobacillus parabuchneri in laboratory animals, in particular mice and rats. They were isolated from the gastrointestinal tract of a wild quail in experimental violation of the intestinal microbial background. Artificial imbalance of the intestinal microbiota of experimental animals was caused by the use of the antibiotic. As a result of tests it was established that the application of probiotic cultures improved microbial disorders of the gut, however, the use of their consortium in the probiotic promoted consistent clinical result characterized by an elevated level of representatives of the normal flora (lacto- and bifidobacteria) and reduction of the number of representatives of pathogenic and conditionally pathogenic microbiota, with subsequent achievement of a level characteristic of healthy animals
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