06.01.05 Selection and seed production of agricultural plants (agricultural sciences)
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TO THE 100TH ANNIVERSARY OF N. I. VAVILOV’S RULE ON HOMOLOGICAL SERIES IN HEREDITARY VARIABILITY
06.01.05 Selection and seed production of agricultural plants (agricultural sciences)
Description100 years ago, on June 4, 1920, 32-year-old Professor of the Saratov University Nikolai Ivanovich Vavilov (1887-1943) first reported at the III all-Russian selection Congress at the University of Saratov on his discovery of homological series in the study of parallelisms in the phenomena of hereditary variability by analogy with homological series of organic compounds. This discovery in genetics received the rank of law, the only one after the laws of G. Mendel. This major study was a further development of the genetic idea of C. Darwin on the origin of species. It showed the ways in which close species and genera of plants have a parallel formative process, because the crucial in the process of evolutionary development of living organisms – first of all, their genetic features. In cases where the development of a trait requires the joint and consistent action of many genes, the occurrence of homological series is inevitable, and this does not contradict the random variability of C. Darwin. In addition to its great genetic significance as a law of evolution, the law of homological series in hereditary variability is of great importance for botanists, plant breeders and breeders: it not only determines the place of each form in the plant world, but can also indicate to the breeder possible directions in his practical work. According to a number of geneticists and breeders, if G. Mendel discovered the rules of heredity, then N. I. Vavilov discovered the rules of variability
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06.01.05 Selection and seed production of agricultural plants (agricultural sciences)
DescriptionThe article presents results of studies on the intraspecific structure of the population of blast causative agent using sets of tester varieties from International Rice Research Institute - IRRI. The differentiation of the fungus population on tester varieties is the most progressive, since it allows one to determine the virulence genotypes of races of pathogens and identify the resistance genes of rice plants effective for them. The work has studied the diversity of blast causative agent in released rice varieties by morphological and cultural traits. Currently, in the world, the resistance of rice plants to blast is becoming one of the important indicators of the competitiveness of varieties. The basis for the successful rice breeding for resistance to blast is the availability of source material with a fairly wide spectrum and a high level of resistance. Resistance donors are selected based on the identification of genes that control this trait in rice
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THE HISTORY OF LILAC (SYRINGA VULGARIS) INTRODUCTION IN SIBERIA
06.01.05 Selection and seed production of agricultural plants (agricultural sciences)
DescriptionTo create the collection of Syringa vulgaris L. cultivars, the Central Siberian Botanical Garden of the Siberian Branch of the Russian Academy of Sciences (CSBG SB RAS) has received from Dr. Z.I. Luchnik green cuttings of 30 cultivars of domestic and foreign selection from the collection of the Research Institute of Horticulture of Siberia named after M.A. Lisavenko, Barnaul (Federal Altai Scientific Center of Agrobiotechnology – FASCA now). Currently, the collection of Syringa vulgaris in CSBG SB RAS has 26 cultivars of 116 tested ones, most resistant to the conditions of Novosibirsk city. The base of CSBG SB RAS’ collection forms 22 cultivars got from Z. I. Luchnik. Descriptions of 3 rare of common lilac cultivars ‘Altaiyskaya rozovaja’, ‘Dafna’ and ‘Kruzhevnitsa’ by Siberian selection of the Research Institute of Horticulture of Siberia named after M.A. Lisavenko from the collection of CSBS SB RAS are given. Lilac's cultivars from the collection of the CSBG were transferred in the collections of the "Kuzbass Botanical Garden", of the Human Ecology Institute of the Russian Academy of Sciences (Kemerovo), to the Botanical Garden of Irkutsk State University, as well as to the Botanical Gardens of Greater Nansi and the University of Lorraine (France). Our introduced cultivars were planted in the Novosibirsk, Krasnoyarsk and Chaoyang (Liaoning Province, China)
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06.01.05 Selection and seed production of agricultural plants (agricultural sciences)
DescriptionThe article discusses the method of selection using markers in the selection of parent pairs for hybridization, in the selection process and in the subsequent evaluation of parent lines, used in linear and backcross selection of tomatoes. The work was performed on tomato hybrids that combine resistance genes to tomato mosaic virus (ToMV) or tobacco mosaic virus (TMV) in their genome. The research used seeds of foreign F1 tomato hybrids recommended by the manufacturer as resistant to the tobacco mosaic virus. Most of the tomato hybrids were Dutch selection, such leading firms as De Ruiter Seeds, Enza Zaden, Rijk Zwaan. The aim of the work was to test a system of hybridization probes designed to identify a gene for resistance to the tobacco mosaic virus. The study of the genotype of tomato plants was carried out in the laboratory of molecular diagnostics of plants , equipped with modern devices for PCR analysis on the basis of NIIOZG (Krymsk). Plant material was collected in different phases of plant vegetation. pre-numbered 1.5 ml test tubes (SSI-1200-00), plastic tablets, tweezers, and distilled water were used to collect plant samples. The collected samples were stored in a refrigerator until the plant DNA was isolated. During the study, collectible tomato samples were studied, including 8 F1 hybrids and 2 lines used as controls. According to the results of genetic analysis using the marker of the TMV resistance gene, it was found that all hybrids and one control line # 175/14 have a resistance gene in their genotype, and the analysis also showed the state of the gene – homozygous or heterozygous. The results of artificial infection confirmed the results obtained by PCR analysis. At the same time, using genetic research, it is possible to determine the exact state of the gene in the plant, which cannot be determined by methods of artificial infection, since no visual differences were found between plants with a heterozygous and homozygous state of the gene. The created marker and PCR analysis method can be recommended for wide application in the selection process of tomato culture. As a result of research, it was found that modern tomato hybrids have a gene for resistance to tobacco mosaic virus (TMV) in their genotype. This was determined by genetic analysis and confirmed by a test using artificial infection
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EVALUATION OF THE SOURCE MATERIAL TO CREATE HETEROTIC HYBRIDS OF TOMATO FOR PLASTIC FOIL HOUSES
06.01.05 Selection and seed production of agricultural plants (agricultural sciences)
DescriptionThe creation of breeding material with desired properties is a principal problem, the successful solution of which is now possible with the introduction of genetic engineering technology. We have carried out an evaluation of the source material on its basis to identify samples suitable for creating heterotic hybrids with a set of economically valuable traits, including resistance to the tomato leaf curl virus (TYLCV)
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ON THE HYBRIDIZATION OF RICE VARIETIES AND WEED-FIELD RED GRAIN FORMS
06.01.05 Selection and seed production of agricultural plants (agricultural sciences)
DescriptionLarge areas under commercial rice in many countries of the world including Russia are infested with weed-field red grain forms of rice. In the nineties of the XX century new red grain forms of rice appeared in the rice commercial fields of the Krasnodar region; these plants differed little from the morphotype of the variety in morphological characteristics. Such plants were difficult to distinguish and remove during varietal weeding. The increased content of red rice grains among the elite seeds leads to the rejection of such crops, thus reducing the economic efficiency of the seed production. Researchers do not have a single opinion on the origin of the new forms of the red grain rice. Various assumptions are made on mutations, unlocking of epigene of the pericarp coloration of the caryopsis to return to the ancestral red grain forms, and cross-pollination between cultivated and red grain rice. In 2014-2018 a methodological experiment on artificial hybridization between the white grain variety and rice varieties with colored pericarp of the grains (Mars and Yuzhnaya Noch) was carried out to discover the origin of these new forms of the red grain rice. The article presents an analysis of hybrids of direct and reverse crosses between the white grain variety Snezhinka, having an amylose long grain, and the black grain variety Yuzhnaya Noch with a glutinous short grain. As a result of artificial hybridization, a new initial material was received with a different type of grain in shape, color and consistency of the endosperm. This material is of significant interest for further breeding and genetic research. When evaluating hybrid plants obtained by direct crossing Snezhinka / Yuzhnaya Noch and reverse crossing Yuzhnaya Noch / Snezhinka, a significant influence of the maternal cytoplasm on the formation of quality signs of endosperm has been revealed. This must be considered when planning such studies. An analysis of the economically valuable traits of the received hybrid material brings a conclusion that the new red grain forms in the commercial rice varieties may appear as a result of spontaneous hybridization between red grain and white grain rice
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06.01.05 Selection and seed production of agricultural plants (agricultural sciences)
DescriptionA significant decrease in yields of field crops due to lack of moisture enhances the role of adaptive breeding. The development of varieties that combine high productivity and quality and resistance to biotic and abiotic stresses is complicated by a negative correlation between high yield and resistance to adverse conditions. Selection of effective methods for assessing the potential of productivity and resistance to stress factors allows creating varieties that provide stable high yields. We had estimated six varieties of common mustard for 10 years in contrasting weather conditions in the zone of insufficient and unstable moisture of the Azov district in the Rostov region. The necessity of taking into account the indicators of the HTC, the index of the environment and its value when conducting an objective analysis of weather conditions is revealed. The greatest stress resistance, ecological plasticity and stability of the Nika variety were established by calculating the difference between the minimum and maximum yield per year, calculating the coefficient of linear regression and the value of homeostasis. The varieties Lera, Donskaya 8, Lux bred in conditions of insufficient moisture demonstrated maximum genetic flexibility and drought resistance. The greatest breeding value was determined in varieties Donskaya 8 and Lera. These varieties are recommended to be included in further breeding work. The study of mustard varieties in contrast conditions (HTC from 0.30 to 1.53) marked their broad environmental plasticity, which makes it possible to recommend them for cultivation in different weather conditions regions
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06.01.05 Selection and seed production of agricultural plants (agricultural sciences)
DescriptionThe review article describes the role of tomato gene mutations in the creation of new cultivars and hybrids suitable for mechanized harvesting. The work gives characteristic of different genes associated with morphological characteristics of tomato plants, types of fruit (flower) brush and peduncle. The genes controlling the shape and size of the fruit, its resistance to cracking and mechanical effects are noted, the value of these genes for the breeding of machine cultivars and hybrids is shown. The article emphasizes the need to create new cultivars and hybrids of tomato suitable for mechanized harvesting with high quality fruits, evenly colored, without green spots at the base, corresponding to the basic requirements of the processing industry. The figures illustrate gene mutants that are of the greatest interest for the breeding of cultivars, suitable for mechanized harvesting
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SPIKE PRODUCTIVITY IMPLEMENTATION IN COLLECTION SAMPLES OF WINTER SOFT WHEAT
06.01.05 Selection and seed production of agricultural plants (agricultural sciences)
DescriptionThe work is devoted to studying the productivity of an ear of winter soft wheat samples from the collection of the All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov (VIR). The study attempted to conduct a pre-selection study of collection material on the elements of spike productivity. The concept of pre-selection research is based on the study of initial collection material according to given characteristics in order to identify the best genotypes for further use in breeding practice. In the analysis of the collection material, the following characteristics were used as indicators of spike productivity: length of the main spike (cm), number of spikelets in the main spike (pcs), number of grains in the main spike (pcs), grain weight from the main spike (g), 1000-kernel weight (g). Plant height was one of the basic indicators of the studied material, because a number of samples differed in alleles of short-stem genes. According to the results of two years of research, according to the indicator «1000-kernel weight», the varietal sample Cologna lunga razza 188 (Italy) was identified as 71,20 g. According to the indicator «number of grains in an ear», the varietal sample Fenotipo 1 (Italy) was 57,3 g. Also according to the totality of indicators, the varietal sample Kaloyan (Bulgaria) with an average of two years of research with a 1000-kernel weight – 51,77 g, the number of grains in an ear – 51,6 pcs. and a mass of grains per ear – 2,71 g.
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SOWING AND CROP PROPERTIES OF SOFT WINTER WHEAT SEEDS DEPENDING ON THE REPRODUCTION ZONE
06.01.05 Selection and seed production of agricultural plants (agricultural sciences)
DescriptionThe article presents the results of studying the influence of the reproduction zone on the sowing and yield quality of seeds of wheat varieties of soft winter selection of the NPC named after P. P. Lukyanenko. From the sowing qualities regulated by the state standard of the Russian Federation, the following indicators were studied in the experiment: viability ( % ) and weight of 1000 seeds (g). The P-1 seeds obtained in the NPC were sown in different agro-climatic zones of the region in the optimal time with the same seeding rate of 250 seeds/m2. The obtained seed material was supplied for the study of sowing qualities and yielding properties of learning in the nursery ground control. The yield quality of seeds significantly differed depending on the area of their production in the previous year. According to the results of four years of research, the seeds of varieties obtained in the Northern zone of the region had higher sowing qualities compared to seeds from other agro-climatic zones. The average seed viability for four years in the Tanya variety was 97.6%, in the Yuka variety-97.7% and in the Grom variety-98.0%. The largest mass of 1000 seeds was also observed in the studied varieties from the Northern zone of the region. Consequently, a more complete realization of the potential of the three studied varieties was also observed in the Northern zone of the region