All-Russian Research Institute of Rice
Author list of organization
List of articles written by the authors of the organization
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REVISITING THE RICE SALT RESISTANCE (REVIEW)
06.00.00 Agricultural sciences
DescriptionThe total area of saline soils of rice irrigation systems in the Kuban region reaches almost 80 thousands hectares. Salinization and alkalinization here become a limiting factor for cultivating rice and companion crops. In this regard, the tolerance of plants to soil salinity is a current problem of plant breeding that attracts the attention of many agricultural researchers whereas it is necessary to increase the yield in saline soils. Salt tolerance of crop plants is defined by a combination of properties which are based on the specific adaptation mechanisms. These mechanisms differ in nature and are associated with different levels of structural organization of the plant – from molecular to organismic one. The study of adaptation mechanisms at different levels of structural organization of plants is very important for increasing yield in saline soils. This article is a review giving a detailed analysis of plant salt tolerance research, particularly rice, which addresses the issues of inhibition of plant growth under saline conditions, the mechanisms of salt tolerance, the achievements and development of this branch of scientific research, as well as the application results of work found in the scientific literature
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APPROBATION OF MULTIPLEX SSRANALYSIS FOR DNA-FINGERPRINTS OF RICE VARIETIES
DescriptionResults of testing of multiplex sets SSR-markers for genotyping of rice varieties are presented in the article. Two sets of SSR-markers were formed: 1: RM1+ RM11+ RM70+RM122; 2: RM164+RM167+RM168. The optimal combination of DNA markers in the multiplex sets and PCR conditions allowed obtaining accurate, easily interpretable results when performing fragment analysis on automated genetic analyzer ABIprism3130. Using multiplex sets, genotyping was performed for several varieties of rice: domestic breeding and one variety – IR36 from the breeding of IRRI (Manila, Philippines). For all the studied varieties specific SSR-fingerprints were obtained. RM 168 marker showed in domestic varieties a low level of polymorphism - one allele of 97 bp. However, at the same time, the variety IR-36, showed a second type of allele 107 bp. In addition, the loci of RM1, RM11, RM167 and RM164 have unique alleles in this variety. It is consistent with significant genetic differences of these varieties and the rest of the varieties in studied sample. The proposed SSR multiplexes are promising for use in DNA certification of rice varieties and assessment of genetic diversity
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SCIENTIFIC BASE OF USING COMPLEX MICROFERTILIZERS IN RICE GROWING
06.00.00 Agricultural sciences
DescriptionThis article is a review work where the role of microelements in life of plants is observed and theoretical and practical issues concerning application of complex microfertilizers enhancing productivity, quality of seeds and grain on rice crops are highlighted. The complexions of the metals used as micronutrients, are an effective form of trace elements and means of regulation of the production process of agricultural crops as seed treatment before sowing, and when conducting foliar vegetating plants. Their inclusion in the system of fertilizer rice to balance mineral nutrients necessary for plant life, providing increased productivity, quality seeds and grains
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Description
In this article, we discuss the results of the research of quality characteristics of Russian and foreign rice varieties. Possibility of using data in characterizing germplasm from collection is discussed
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VARIETY OF MICROORGANISMS GROUPS LIVING ON BERRIES OF GRAPES
DescriptionThe wide variety of microorganisms has been identified in many wine-making countries on the berries of grapes. These are yeasts of different families, forms and kinds, bacterium, mold fungi. In the article, we present the results of investigating species composition of microflora of berries of white and red types of grape, which grows in different economies of the Krasnodar region and the republic of Abkhaziya. The sowings onto the elective media were conducted for the development of entire spectrum of yeast. The grown colonies after preliminary microscoping were separated into the cultures and subjected to testing according to the culturalmorphological signs, being guided by determinants and benefits. It was established the specific variety of microflora on the surface of the berries of grapes of all investigated types, without dependence on the place of their growth. Obtained data showed that the group of yeast, which constantly is present in the complex of the epiphytic microorganisms of grapes of Saccharomyces, Pichia, Hansenula, Hanseniaspora was characteristic for all types of grapes in all investigated regions. The heterogeneity of the taxonometric composition of microflora is shown. Prevailed yeasts were of family Saccharomycetaceae, form Saccharomyces vini. A quantity of yeast of Saccharomyces vini decreases in a number of Myskhako-Caucasus-Fanagoriya, that as a whole will be coordinated with the climatic conditions. Only the type of Pinot nuar grapes had yeasts of Brettanomyces Dekkera. On the berries of Cabernets and Karaburnu we have discovered yeasts of Schisosaccharomyces acidodevoratus, causing acid-reduction. On the berries of the grapes, which grew in joint stock company APF “Fanagoriya” we haven’t revealed the presence of lactic acid bacteria Lactobacillus brevis and yeasts of the form of Schisosaccharomyces acidodevoratus. In the same farm the smallest quantity of yeastswreckers is noted, which we the forms of Pichia and Candida. In the microflora of Cabernets, Risling and, especially, Karaburnu grapes, we have found lactic acid bacteria Lactobacillus plantarum and Lactobacillus brevis. Lactobacillus plantarum. Among the bacteria the total quantity of coccic flora composes - 56 %, the rod-shaped - 44 %. Two types of active acetous bacteria are identified – Acetobacter aceti and Acetobacter xelinum. Especially high was their surface concentration on the berries of Kachich type of grapes
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Description
The study was performed to genotype some commercial wine yeast strains using the assay of Interdelta genomic sequences. Experimental parameters of PCR to identify were optimized and optimal simplified method of DNA extraction from dried preparations of yeast cultures was define. Proven method showed a high level of resolution and can be used for the analysis of genetic diversity wine yeast in combination with SSR-markers
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06.00.00 Agricultural sciences
DescriptionIn this article the results of the research of quality characteristics of Russian and foreign rice varietieswere observed. A possibility of using the data in characterizing germplasm from the collection was discussed
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06.00.00 Agricultural sciences
DescriptionThis article reviews hybrid analysis data on monogenic and digenic patterns of quantitative traits: plant height, panicle length and total number of spikelets
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06.00.00 Agricultural sciences
DescriptionMultiplex DNA-marker set for PCR identification for rice blast resistance genes Pi-40 and Pi-b was developed in this study. Optimal primers combinations and PCR conditions allows to identify both abovementioned genes in the single PCR
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Description
In the process of the research we had accomplished an analysis of allelic polymorphism of self-incompatibility in pears in demand in the production and breeding of modern varieties of pears of North-Caucasian Zonal Research Institute of Horticulture and Viticulture collection of genetic resources. In the first stage the consensus primers PycomC1F and PycomC5R were used. With obtained data, after identification of the alleles studied in varieties using consensus primers an allele specific S5 and S8 DNA markers were used for confirming the presence / absence of data allele studied cultivars