Krasnoyarsk State Agricultural University
Author list of organization
List of articles written by the authors of the organization
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ESSENCE OF ECONOMIC POTENTIAL AND A SYSTEM OF INDICATORS OF ITS ASSESSMENT
DescriptionIn the market conditions, the key to survival and the basis of the provisions of the stability of any business entity is financial stability. Its achievement is possible by improving the efficiency of economic activities, which is achieved when the rational use of all kinds of resources and reduce costs. The important role in realization of this task has been given to the economic potential of the control system. Therefore, the economic potential of the analysis and evaluation of the topic, i.e. efficient use of all resources, is relevant at this stage of development of the domestic economy. Studying the nature resources as a basis for economic development, identifying their sources, development of ways, forms and methods of their use allow us to formulate the principles of activation of economic policy for the intensification of economic processes. In the conditions of growing complexities of the Russian economy the definition of resource capacities should be a priority in the development strategy of any business entity, region, industry. One of the most important branches of the material production of the Krasnodar region is agriculture. Today's reality is that the consumption of many food products is still below the science-based standards, and import dependence exceeds the allowable criteria of food security, despite the fact that the number of rejected and lowered in grade imported food products is quite large
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THE STUDY OF GRAPE GENETIC DIVERSITY OF THE NORTH CAUCASUS GENE POOL
06.00.00 Agricultural sciences
DescriptionThe article presents the results of studies of 92 indigenous varieties and 15 wild genotypes by 24 microsatellite markers. As a result a locus database about germplasm in the collection stored at the Russian ampelographical collection AZOSViV (Anapa Zone Experimental Station of Viticulture and winemaking) was created. During the research of varietal breeding populations confirmed the validity in the production of the cultivar of Krasnostop anapsky with obvious changes in microsatellite sequences as a clone of famous indigenous Don cultivar Krasnostop Zolotovskii. This clone was selected in 1953 by prominent agrotechnologist Zotkin Ivan Ivanovich which is propagated by the author and his followers, and after a formal registration submitted to state testing in 2007 in GSK RF, zoned in the North Caucasus region (number 6) in 2014 year. Sort -clone Krasnostop anapsky only in the Kuban today cultivating in 35 hectares. By using molecular genetic techniques, now clonal selection of vine grapes is reasonably improving. Recent data collected by us in the work process, were analyzed a comprehensive manner, making it possible to draw conclusions about distinctness of grape varieties. Some clones were studied by DNA primers, based on retrotransposon sequences
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EXTRACTING DNA BY NUCLEOSPINPLANT 2 COREKIT. COMMENTS
DescriptionThis review article highlights a new method of DNA extraction from herbarium leaves of grapes for use in PCR-analysis
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STRUCTURAL AND PHYLOGENETIC ANALYSIS OF VVMYBA1, VVMYBA2 AND VVMYBA3 GRAPEVINE GENES
DescriptionThe article presents the structural and phylogenetic analysis of VIT_02s0033g00410, VIT_02s0033g00390 and VIT_02s0033g00450 genes of the grapevine genome and the closely related orthologous gene MYB114 of the Arabidopsis genome. These genes are responsible for the biosynthesis of anthocyanin in the organs of model plants and are of interest not only for practical production and breeding, but also for fundamental research. These genes were analyzed for GCcomposition of nucleotides, the presence of cisregulatory elements and promoter regions. DNA and protein sequences were aligned to look for similar elements, which allowed further analysis of the ultraconservative domains of four genes. Based on the results of search and identification of the conservative regions, a cluster tree was constructed, which made it possible to identify the separation of gene sidelines from, presumably, the main one. At the same time, the construction of consensus trees based on DNA and protein sequences revealed their absolute similarity. "The Minimal Evolution Tree" allowed calculating the approximate dates of the appearance of the mutations and the divergence times of the gene branches between each other. At the same time, the appearance of the Vitis genus and its separation from the Rosales was taken as the time first divergence point. In the end, homologous metabolic pathways were searched between grapevine and Arabidopsis, which revealed the presence of homologous proteins in the grape proteome. In this turn, it already confirms the existence of similar biosynthetic pathways and, as a consequence, interactions such as "DNA-protein" and "protein-protein"
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06.00.00 Agricultural sciences
DescriptionIn the present article, we have described data of comparative ampelography of biometric evaluation of leaf parameters of the three table grapes: Preobragenie, Victor and Jubiley Novocherkassk, widespread in the amateur and farming areas of Russia and the Ukraine. Showed results of molecular genetic analysis of DNA from these table grapes
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GENOTYPING OF GRAPE VARIETIES WITH USING MICROSATELLITE SSr MARKERS
06.00.00 Agricultural sciences
DescriptionThis article presents the results of genotyping of new perspective table and technical varieties and grapes protoclones. It was established that varieties of Citrine, Helios, Arcadia pink and Preobragenie show differ genetic diversity in four loci
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IMPROVEMENT OF WATER SUPPLY OF FRUIT PLANTS
06.00.00 Agricultural sciences
DescriptionIt is established that in a vegetative experience the most effective way to water supply was application of AquaLife supersorbent granules
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EXTRACTING DNA WITH USING PEQGOLD PLANT DNA MINI KIT
DescriptionIn the present article, the new method of DNA extraction from fresh and herbarium leafs of grape to their subsequent sequencing was described
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IMPACT ON WAYS OF WATER SUPPLY BIOMETRICS APPLE SEEDLINGS
06.00.00 Agricultural sciences
DescriptionThis article has explored different ways of watering annual apple seedlings in pots. The greatest phytomass was obtained with pellets of Aqualife supersorbents in the version of 10gr. dosage
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STUDY OF INDIGENEOUS RUSSIAN GRAPE VARIETIES USING MICROSATELLITE MARKERS
06.00.00 Agricultural sciences
DescriptionThe analysis of genetic polymorphisms of 12 autochthonous grape varieties grown in the National ampelographic collection of Russia (Anapa district of the Krasnodar region) through the study of allelic diversity at six microsatellite loci: VRZAG79, VVMD5, VVMD7, VVMD27, VRZAG62, VVS2 has been done. We have found that all native varieties have a unique set of allele. The assessment of genetic relationships varieties has been performed using cluster analysis. Data for DNA certification of the investigated genotypes of the grapes has also been obtained in the article