name
Zvyagin Andrey Sergeyevich
Scholastic degree
•
Academic rank
—
Honorary rank
—
Organization, job position
• Kuban State Agrarian University
Research interests
-
Web site url
—
Current rating (overall rating of articles)
0
TOP5 co-authors
Articles count: 15
Сформировать список работ, опубликованных в Научном журнале КубГАУ
-
06.00.00 Agricultural sciences
Description
The polymorphism is the diversity of varieties in ampelografy - the presence simultaneous permanent in the population of two or several phenotypic and genotype differing groups of plants of one variety – i.e. clones. In viticulture the isogenic population is usually called “clone” and variety as the total of clones or forms – i.e. it is heterogenic. Polymorphism of varieties has big practical sense, as varieties-clones have the harmonic complementary adaptive ampelocenosis and higher economical effect for production. In the article the polymorphism of two heterogenic population - the group Pinot and Riesling, which are the best specimen of French and German viticulture is described on the phenotypic level of leaves
-
EXTRACTION OF DNA FROM HERBARIUM LEAVES VITIS VINIFERA L.
06.00.00 Agricultural sciences
Description
Two methods were used for DNA extraction from herbarium leaves of two subspecies: V. vinifera subsp. sativa D.C. и Vitis vinifera ssp. silvestris Gmel. DNA extraction is difficult from herbarium leaves plants because of the presence of metabolites that interfere with DNA isolation procedures and downstream applications such as DNA restriction, amplification, and cloning. The quality of DNA was checked on a spectrophotometer by measuring the level of absorption at A260/280 nm. The results showed the successful extraction from mature and dried leaves of DNA when using the СТАB-method №2. Another method hasn’t shown satisfactory results in amplification of DNA
-
EXTRACTION OF DNA FROM LEAVES OF VITIS VINIFERA L.
06.00.00 Agricultural sciences
Description
Extraction of the DNA from leaves of genus Vitis is a very difficult task, as a grape has a lot of elements, which make difficult to use it in the genetic manipulation. In that article used the methods of extraction of the DNA with silica (SiO2) particles as the absorbent material for getting better quality DNA
-
GENOTYPING OF NEW PERSPECTIVE WINE GRAPES WITH USING MICROSATELLITE MARKERS
06.00.00 Agricultural sciences
Description
In this article we have described our work on genotyping of new ten productive wine grape protoclones
-
GENOTYPING OF THREE NEW PRODUCTIVE WINE VARIETIES WITH USING MICROSATELLITE MARKERS
06.00.00 Agricultural sciences
Description
In this article we have described a study of genotyping of new three productive wine grapes protoclones: Rhine Rieslin, Verdot black and Johanniter
-
THE STUDY OF GRAPE GENETIC DIVERSITY OF THE NORTH CAUCASUS GENE POOL
06.00.00 Agricultural sciences
Description
The article presents the results of studies of 92 indigenous varieties and 15 wild genotypes by 24 microsatellite markers. As a result a locus database about germplasm in the collection stored at the Russian ampelographical collection AZOSViV (Anapa Zone Experimental Station of Viticulture and winemaking) was created. During the research of varietal breeding populations confirmed the validity in the production of the cultivar of Krasnostop anapsky with obvious changes in microsatellite sequences as a clone of famous indigenous Don cultivar Krasnostop Zolotovskii. This clone was selected in 1953 by prominent agrotechnologist Zotkin Ivan Ivanovich which is propagated by the author and his followers, and after a formal registration submitted to state testing in 2007 in GSK RF, zoned in the North Caucasus region (number 6) in 2014 year. Sort -clone Krasnostop anapsky only in the Kuban today cultivating in 35 hectares. By using molecular genetic techniques, now clonal selection of vine grapes is reasonably improving. Recent data collected by us in the work process, were analyzed a comprehensive manner, making it possible to draw conclusions about distinctness of grape varieties. Some clones were studied by DNA primers, based on retrotransposon sequences
-
06.00.00 Agricultural sciences
Description
Problems of biometric evaluation of morphological features of clones for identification of variety group Merlo genotypes were considered in the paper.
-
STUDY OF INDIGENEOUS RUSSIAN GRAPE VARIETIES USING MICROSATELLITE MARKERS
06.00.00 Agricultural sciences
Description
The analysis of genetic polymorphisms of 12 autochthonous grape varieties grown in the National ampelographic collection of Russia (Anapa district of the Krasnodar region) through the study of allelic diversity at six microsatellite loci: VRZAG79, VVMD5, VVMD7, VVMD27, VRZAG62, VVS2 has been done. We have found that all native varieties have a unique set of allele. The assessment of genetic relationships varieties has been performed using cluster analysis. Data for DNA certification of the investigated genotypes of the grapes has also been obtained in the article
-
THE RESEARCH OF HAPLOTYPE DEVERSITY OF RUSSIA ABORIGINAL GRAPE VARIETIES
06.00.00 Agricultural sciences
Description
In the article we have studied 55 aboriginal varieties, collected in the North Caucasus. We have described allelic diversity and identified four haplotypes. All the populations were closely related while being examined using a factor of Nei's genetic distance (Dn), with the exception of indigenous varieties of Armenia. Maximum differences have been found between the Uzbek population and Astrakhan, Turkmenian, Armenian populations. The most genetically rich were the population of Don area and Krasnodar region
-
THE RESEARCH OF VITIS SILVESTRIS GMEL. IN THE NOTHERN CAUCASUS
06.00.00 Agricultural sciences
Description
Vitis vinifera subsp. silvestris Gmel. is the wild subspecies of Vitis vinifera L. and an important source of genetic resources for grapes breeding. Currently, this form is very rare in the world. In this paper, conducted the first study of wild grapes which was found in the North Caucasus with the use of two microsatellite markers. The study have shown a high level of genetic diversity: intrapopulation variability was 75,2%, the interpopulation variability accounts was 24,8%. All populations are characterized by certain unique genetic structure and may be as donor of resistance genes which have a long-term evolutionary selection
pdf 164.506kb
doc 164.506kb