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ACCELERATED DIFFERENTIAL INDICATION OF VEGETATIVE AND L-FORMS OF MICROORGANISMS
Description
На основе дифференциального концентрирования бактерий с помощью сендвича фильтров с различным диаметром пор и ПЦР-РТ разработана схема индикации биологических агентов бактериологической природы, находящихся в L-трансформации
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DNA TECHNOLOGY OF PCR ANALYSIS OF CODLING MOTH POPULATIONS BY MICROSATELLITE LOCI
Description
The DNA technology of the PCR analysis of codling moth populations by microsattelite loci is adduced in detail. It includes a list of the equipment, consumable materials and chemical agents, methods of DNA extraction, PCR analysis and electrophoresis of amplification products in polyacrilamid gel. The example of the PCR analysis of codling moth population from Krasnodar region with two microsattelite loci is adduced
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06.00.00 Agricultural sciences
Description
Using two microsatellite loci (HaSSR1, HaSSR3), the PCR analysis of the bollworm Krasnodar population was conducted. Molecular-genetic analysis (SSR-PCR) of different geographic codling moth populations by three microsatellite loci Ср1.63; Ср2.39 и Ср2.157 was conducted also. Based on the assessment of the frequencies of occurrence of DNA markers, the molecular-genetic structure was described and genetic diversity in the pest populations was estimated
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