03.00.00 Biological sciences
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Description
In the article, we have proposed regional environmental standards for heavy metals, oil and oil products in the main soils of southern Russia
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DEVELOPMENT OF OPTIMAL WAY OF OBTAINING A HYDROLYZATE OF LACTIC ACID BACTERIA
DescriptionThe work was carried out in the scientific-research laboratory of the chair of Biotechnology, Biochemistry and Biophysics of Kuban SAU, the aim of which is the search of optimal conditions of obtaining the hydrolyzates of lactic acid bacteria by thermoacid and enzymatic ways. The pure cultures of microflora of gastrointestinal tract of quails − Lactobacillus agilis, Lactobacillus intermedius and Lactobacillus salivarius were the object of researches. Under conducting the thermal acid hydrolysis there were used the same correlations of bacterial mass and water as well as procedures of clearing of hydrolyzates. The variable conditions were рН, temperature and time of hydrolysis. When carrying out the enzymatic hydrolysis in this study there was used the enzyme from the group of muramidase lysozyme. The amount of enzymes and time of carried out hydrolysis were the variable conditions under obtaining the enzymatic hydrolyzates. In the process of researches there were studied: qualitative content of total protein, peptides with М.м. < 1500, protein and peptides with M.m. > 1500, aminoacids and as well as GMDP (glucosaminilmuramilpentapeptide). On the basis of carried out experiments there was stated that according to all components of biologically active substances the thermal acid way is more effective than enzymatic. The maximum accumulation of BAA in hydrolyzates by different ways was revealed at destruction of cells Lb. intermedius. Content of GMDP in thermal acid hydrolyzate was higher than in enzymatic on 0,028 g/100 ml (51,8 %)
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Description
The article considers the new method for recovery of P. arbelaeziion intact material on the basis of grafting into endogenous pathogens resistant Passiflora species in vivo. The influence of the explants origin depending on the stock of intact plants on the development of regenerated P. arbelaezii under in vitro condition is examined
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DEVELOPING OF THE METHODOLOGY FOR THE IDENTIFICATION OF PICEA ABIES L. CLONES BY USING ISSR MARKERS
DescriptionThe technology for the identification of Picea abies L. clones by using ISSR markers was developed. 9 ISSR primers ((GA)9T; (AC)8C; (CA)6RY; (CA)6RG; (CA)6(GT); (CA)6(AC); (AG)8T; (GA)8С и (AG)8YT) were recommended to use for genotypes detection. The selected primers have shown the high level of polymorphism. A total of 172 amplified fragments were found by the selected primers and 149 (86.6%) of them were polymorphic
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Description
In the article we have conducted the estimation of breeding population of salix acutifolia. We have studied the following indicators of the measured trees: the percentage of salicylates, bark content in relation to the wood. For these two indices we have calculated the "complex" productivity of the trees. Based on that, the trees are assigned to these breeding categories: Category 1: trees with high productivity - ( 49.7 - 39.9 kg / t of raw materials) Category 2 : trees with average productivity - ( 39.1 - 33.8 kg / m raw materials) category 3 : trees with low productivity - ( 31.8 - 24.3 kg / t of raw materials)
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DEVELOPMENT OF PROTEIN ENZYME FEED ADDITIVE FOR POULTRY FARMING
DescriptionThe research work was conducted at the Research Laboratory of Biotechnology, Biochemistry, Biophysics Department of Kuban State Agrarian University. The main purpose of this study was to select the most productive type of fungus of a genus of Trichoderma and optimal nutrient medium on basis of a waste of reprocessing of soy for acquire of protein enzyme feed supplement. For selection of a fungus of a genus Trichoderma used 3 types: Trichoderma viride, Trichoderma lignorum, and Trichoderma harsianum. We used 3 types of nutrient media as a carrier for micromycete, which were based on soy okara and the additional sources of reprocessing of raw plant material – husk of a sunflower, husk of wheat (bran) and husk of rice. The results demonstrated the benefits of using the nutrient medium based on soy okara forms and husk of a sunflower fungus to enhance Trichoderma lignorum production
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Description
In the article we present the results of comparative researches of herbaceous covering of plant communities in the alpine zone of the Republic of Dagestan on the example of Dido- depression. The plant covering of the investigated region differs in extraordinary variety. The general regularity of plant distribution is vertical zones. There were made 6 ground profiles at different elevations from 1450m to the alpine zone at 2500m above sea level. Variety of phytocenoses is connected as with changes of ground conditions, so with environmental conditions. We have researched and identified the types of grounds with laying of grounds profiles on the vertical zonality. There we studied the specific composition of herbaceous plants on the examined ground and given names to plant communities. The studying phytocenosis are analyzed by productivity of functional groups. We have shown the share of each species and their abundance. Of all plant communities, the mass of herb differ dominates. The composition of plant communities is analyzed, depending on high-altitude grades and degree of anthropogenic load influence
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VARIETY OF LEGUMES IN NATURAL COMMUNITIES OF THE YAKUT BOTANICAL GARDEN
DescriptionThe article provides the information about the species diversity of the family Fabaceae in the natural area of the Yakut botanical garden. It also provides the information about the method and the study area, a description of the ambient phytocenotic, taxonomic analysis, areal analysis, bio-morphological analysis (the variety of life forms) given by K. Raunkier, rhythm analysis, environmental analysis (by A.P. Schennikov), analysis of phytocoenotic. We have also provided a summary of the endemics of Central Yakutia - Thermopsis lanceolata R. Br. subsp. jacutica (Czefr.)
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A VARIETY OF ASTERACEAE IN NATURAL COMMUNITIES OF THE YAKUT BOTANICAL GARDEN
DescriptionThe article provides information about the species diversity of the family of Asteraceae in the natural area of the Yakut botanical garden. We provide the information about the method and the area of the study, the description of the phytocenotic surrounding, taxonomic analysis, areal analysis, bio-morphological analysis (the variety of life forms) which was given by the K. Raunkier, rhythmological analysis, environmental analysis (by A.P. Schennikov), analysis of phytocoenotic
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RADIOECOLOGICAL RISKS OF BOVINE LEUCOSIS SPREAD IN CHELYABINSK REGION
DescriptionRadioecological risks of bovine leucosis spread among cattle in Chelyabinsk region are estimated. The obtained data can be used for simulation modeling and the forecasting of leucosis prevalence in the conditions of ecological troubles