06.02.00 Veterinary and Husbandry
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CORRECTION OF HOMEOSTASIS OF THE ORGANISM OF LARGE CATTLE AT THE NODULAR DERMATITIS
06.02.00 Veterinary and Husbandry
DescriptionWe have studied biochemical changes in the body of cattle, sick with nodular dermatitis in the initial stage of the clinical manifestation of the disease. It is proved that in case of disease in animals there is a significant increase in the level of creatinine up to 295.5 mg%. The excess of the norm by 82.1%. In treatment, this indicator was significantly reduced (189.2 mg%), but did not reach the upper limits of the norm (162.4 mg%). In the blood serum of patients, the carotene content was reduced to 0.09 mg%, which was restored to normal three days after the start of treatment (1.0 mg%). The glucose level at the beginning of the disease was 18% below normal and reached the lower limit of the norm 8 hours after the start of treatment. When studying the activity of CKK, it was found that the activity of the enzyme in animals before the introduction of a 5% solution of sodium hydrogencarbonate is 82% higher than the upper limit of the norm. After the treatment, this indicator returned to normal. The inorganic phosphorus index did not reach the physiological norm (2.5 mmol / l) before and after treatment, and remained 11.6% higher (2.9 mmol / l), which indicates a violation of mineral metabolism in sick animals. The level of reserve alkalinity began to recover after 8 hours of observation and reached a minimum limit of 48.91 vol. % CO2. During the entire period of the experimental studies, before and after the administration of the drug, there were no significant changes in the biochemical parameters of total protein, calcium, vitamin E and urea. The nature of the established changes varied within the limits of physiological norms. The use of a 5% solution of sodium hydrogen carbonate in nodular dermatitis increases the effectiveness of symptomatic treatment, reduces the likelihood of complications and stimulates the production of specific antibodies against viruses and bacteria. We have recommended intravenous injection of 5% sodium hydrogencarbonate solution to sick animals at the rate of 1 ml per 1 kg of live weight of the animal three times with an interval of 3 days
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06.02.00 Veterinary and Husbandry
DescriptionThe researchers obtained data on the morphological, biochemical and trace element composition of the blood of bull-calves of Hereford breed of different sizes. In this regard, scientists and business executives have three natural questions: 1) whether it is possible to predict the size and thus the meat productivity of bulls using these blood indicators; what are the strength and direction of the influence of certain values of blood indicators on the size and weight of bulls; what blood indicators are similar in meaning, and what are different and how much (to what extent). The article is devoted to the reasoned answers to these questions by applying modern methods of mathematical and numerical modeling to solve the corresponding problems. The results of the study can be used by anyone, due to the fact that Eidos the universal automated system, which is a tool of ask-analysis, is in full open free access on the author's website at: http://lc.kubagro.ru/aidos/_Aidos-X.htm, and numerical examples of solving the mentioned problems with the use of artificial intelligence technologies are placed as a cloud Eidos-application #133
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MONITORING OF ANTIBIOTIC RESISTANCE OF ENTEROBACTERIA
06.02.00 Veterinary and Husbandry
DescriptionRelative to the emergence and wide extension of an antibiotic resistance in infectious agents of animal infectious diseases, the particularly important assessment increases over change of sensitivity of various groups of microorganisms to anti-infective drugs. Results of studying antibiotic sensitivity of the enterobacteria isolated from an intestine of sick calves, and dynamics of its change within three years are presented in article. Results of the research demonstrate prevalence of resistance to the majority of anti-infective drugs (AID) among the Enterobacteriaceae isolates emitted from calfs. To chloramphenicol, neomycin and streptomycin from 100% of isolates E. coli, Proteus, Enterobacter, Morganella, Citrobacter were sensitivity. From AID of group of aminoglycosides gentamycin, from group of quinolones – enrofloxacin was the most effective. The good sensitivity to a polymyxin was shown to 80 % of isolates E. coli, Citrobacter spp., Klebsiella spp. It was established that 66,7 % of E. coli isolates, 57,1 % of Enterobacter spp., 75 % of Morganella, 60 % of Edwardsiella, 42,9 % of Citrobacter spp., allocated from sick calfs, has fastness to 7 AID. At the same time to other AID of a series the appreciable number of multiresistant strains shows weak sensitivity. The tendency of augmentation of number of antibiotic resistance isolates to AID which are widely applied in veterinary practice as time passed. High frequency of the combined resistance to traditionally used antibiotic drugs – to kanamycin (up to 80 %), to tetracycline (up to 85 %) and to chloramphenicol (up to 8 3%) testifies to need of obligatory test for sensitivity to AID before their use
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06.02.00 Veterinary and Husbandry
DescriptionIn this work, we have found shortened particle forms of the filamentous bacteriophage Pf, when the lysis of the Pseudomonas aeruginosa PAO1 strain was induced by extracts of bison feces to study the intestinal bacteriophages of this animal. The sizes of the shortened particles from 250 to 950 nm and their thickness 17 nm were determined. The particles had a slightly pointed one end and sometimes a flag on the other extended end. The particles were formed in the process lysis of this strain by other bacteriophages. These lysates also contained a high concentration of vesicles from the pseudomonad membrane. The article discusses the mechanisms of prophage induction in the Pseudomonas aeruginosa PAO1 strain due to phage-phage induction and substances of the investigated extract
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06.02.00 Veterinary and Husbandry
DescriptionProviding the population with basic foodstuffs, sources of proteins, fats, carbohydrates, vitamins and other essential nutrients is one of the main issues of ensuring the security of the country, while the requirement for food safety is paramount. Production of feed additives (concentrates), which improve feed conversion, while being natural and safe for animal health, acquires the greatest importance in the conditions of intensification of agriculture and increasing the competitiveness of livestock farms. Probiotic microflora, which, in competition with pathogenic, displaces the latter from the gastrointestinal tract, can be considered as one of the effective methods of controlling undesirable microflora in the digestive tract. In world science, research is actively conducted in the direction of searching for effective probiotic cultures. A promising way to increase the effectiveness of the use of probiotic feed additives is their addition to prebiotics, for example, including vitamins (antioxidants) and mineral substances (selenium). The purpose of this work was to determine the optimal ratio of biologically active substances contained in the feed vitamin-mineral concentrate, and probiotic microflora contained in the feed probiotic additive Bacell-M, for the development of complex feed concentrate. Using the Harrington's desirability function, the optimum ratio of biologically active substances and probiotic microflora in the recipe of the complex feed concentrate is determined, ensuring the maximum reduction in the serum levels of alanine aminotransferase in the blood serum of experimental animals, which characterizes the normalization of barrier organs
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06.02.00 Veterinary and Husbandry
DescriptionProviding the population with basic food products, sources of proteins, fats, carbohydrates, vitamins and other essential nutrients is one of the main issues of ensuring the security of the country, while the requirement for food safety is paramount. Production of feed additives (concentrates), which improve feed conversion, while being natural and safe for animal health, acquires the greatest importance in the conditions of intensification of agriculture and increasing the competitiveness of livestock farms. Probiotic microflora, which, in competition with pathogenic, displaces the latter from the gastrointestinal tract, can be considered as one of the effective methods of controlling undesirable microflora in the digestive tract. In world science, research is actively conducted in the direction of searching for effective probiotic cultures. A promising way to increase the effectiveness of the use of probiotic feed additives is their addition to prebiotics, for example, including vitamins (antioxidants) and mineral substances (selenium). The purpose of this work was to determine the optimal ratio of biologically active substances contained in the feed vitamin-mineral concentrate, and probiotic microflora contained in the feed probiotic additive Bacell-M, for the development of complex feed concentrate. Using the Harrington's desirability function, the optimum ratio of biologically active substances and probiotic microflora in the recipe of the complex feed concentrate is determined, ensuring the maximum reduction in the serum levels of alanine aminotransferase in the blood serum of experimental animals, which characterizes the normalization of barrier organs
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FEATURES OF ANGIOGENESIS OF SQUAMOUS CELL PAPILLOMAS OF DOGS
06.02.00 Veterinary and Husbandry
DescriptionThe article deals with angiogenesis of squamous papillomas of dogs, which is a process of formation of blood vessels, providing neovascularization of tumors with their progression. The differentiation of papillomas depending on morphological and histological features was carried out, progressive and hyperkeratinized types were identified, which determine the stage of development and transition from one to another in the process of tumor growth. It is noted that with the progression of papillomas, the area of the constituent elements of the tumor increases, both due to the stroma and due to the parenchyma, but the stromal-parenchymal ratio changes in the direction of increasing the parenchymal component. Analysis of angiogenesis showed heterogeneity of the isolated vessels, both within the stromal component of papillomas, and depending on the type of tumors. There are three types of vessels – small, medium and large, there is a similar ratio of different types of vessels, regardless of the type of papillomas. Localization of vessels in the stroma depending on belonging to the selected species was studied. The total area of the section of blood vessels with cancer increases, and the ratio of the total area of the section of blood vessels to the area of the stroma is almost at the same level, which determines the dependence of the increase in the stromal component of papillomas from angiogenesis
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SPECIAL ASPECTS OF DIAGNOSTICS, TREATMENT AND PREVENTION OF TURKEY SMALLPOX
06.02.00 Veterinary and Husbandry
DescriptionSmallpox of turkey is a viral, slowly extending disease. It pathogenetically proceeds in a dermatotropic way, skin lesions are characterized primarily by two signs or focal necrotic focus centers or diphtheritic inflammation of mucous membranes, often of the upper respiratory tract. Etiological factor in smallpox are dermatotropic epitheliogenic viruses, the family of Avipoxvirus of Poxviridae. The purpose of this work was studying the features of clinic and the pathomorphological changes at smallpox at turkeys in the context of diagnostics methods and also generalization of treatment-and-prophylactic actions at this pathology. The diseased bird is the main source of infection. In addition, the smallpox virus particles can be spread by people providing care for the birds, indoor air, food, bedding material, excrement. Diagnosis of smallpox takes into account the symptoms; if an autopsy was performed, the result of a pathological pattern as well. Additionally, we can conduct a research of histological sections. Confirmation of diagnosis can be performed by a virological study and the blood serum. In accordance with the pathogenesis of smallpox, there is a classic skin form and diphtheritic. There may be a combination of the above manifestations of smallpox, therefore we can record the mixed form, in some cases, it may be atypical. Pathoanatomical changes come down to defeat a respiratory system of bodies, the upper airways, at the complicated forms there may be noted lesions in the internal organs. Laboratory methods include identification of the virus in the pathological material, as well as additional histological and virological studies using chicken embryos and bioassays. Treatment is difficult and not always effective. The main preventive measure against smallpox in turkey is vaccination, basic applied biologics are: Avivac-pox" strain "K", "Ospovat, Vectorman FP-MG". Prevention of smallpox includes the implementation of general veterinary and sanitary rules in conjunction with the mandatory special prevention. When planning measures to prevent the spread of smallpox, conditions are created to eliminate pathways of transmission of the pathogen – disinfection, disinsection and deratization, as well as vaccination of all susceptible livestock
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ASSESSMENT OF YOUNG PIGS ON THEIR OWN PRODUCTIVITY AND QUALITY OF SPERM
06.02.00 Veterinary and Husbandry
DescriptionA comprehensive assessment was made of the own productivity of repair pigs and the quality of sperm production of boars of different breeds. Imported boars and mumps had thin bacon 13-15 mm, high early ripeness 145-153 days and a depth of "muscular eye" 53-53.9 mm, indicating an increase in the content of lean meat in the carcass. However, the length of the trunk of the animals was 120 cm, which corresponds to the first class of the instructions for bonitation. There was a significant difference in the growth rate of repair young (about 300 g), which indicates the individual characteristics of animals and the possibility of selecting the best animals for reproduction of the herd. Assessment of pigs by phenotype is an important element of breeding work on complexes. It is established that the quality of sperm production is affected both by the breed and the season of the year: the volume of ejaculate and the concentration of spermatozoa in all breeds are greatest in the autumn-winter period. Boars Maxgro were distinguished by an increased volume of ejaculate, but a low concentration of sperm compared to a large white breed and landrace. During the year, the greatest fluctuations in sperm concentration in large white breed boars were observed ± 133 million / ml, the smallest - Landrace ± 29 million / ml, which contributes to the uniform production of semen doses for insemination
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NEED OF DAIRY CATTLE IN ESSENTIAL AMINO ACIDS
06.02.00 Veterinary and Husbandry
DescriptionThe article presents a factorial model for determining the needs of lactating cows in essential amino acids. The algorithm of the model uses fragments from the NRC – 2001 models [39], CNCPS – 200 [60], and research materials published in the world literature. Instead of the transformation coefficients of the metabolizable lysine and methionine for milk production equal to 0.85 and 1.00, respectively, by the CNCPS, the coefficients 0.68 and 0.66 were used according to Doepel et al., 2004 [49] and the authors' own data [69]. Norms obtained using this model in lysine and methionine in milk production and maintenance in percentage of metabolizable protein (MP) were 7.28 and 2.4%, which is identical to the NRC – 2001 standards, equal to 7.2 and 2.4 % respectively, obtained by dose – response method based on dozens of experiments. The norm of histidine was 3.5%, which closely corresponds to the indicator of 2.4 and 2.7 % MP [74], obtained by the incremental addition method. This indicates that the presented model is distinguished by sufficiently high accuracy and is comparable with the models developed by the dose – response method. However, the determination of the need of cows for amino acids in this model is much less expensive than the dose – response method. The need for the absolute amount of metabolizable essential amino acids (MEAA) for milk production (35 kg/d, yield milk protein 1103 gytt6) and maintenance cow - 600 kg, g/d: lysine - 178, methionine - 59, arginine - 119, histidine - 60, isoleucine - 138, leucine - 248, phenylalanine - 152, threonine - 134, tryptophan - 38, valine - 174; the need for only milk production, g/d: 130; 42; 81; 42; 95; 175; 98; 74; 25; 112; the need for only maintenance, g/d: 50; 16; 38; 20; 43; 73; 54; 60; 14; 64; the need for 1 kg of milk (31.5 g of protein) g/kg: 3.7; 1.2; 2.3; 1.2; 2.7; 5.0; 2.8; 2.1; 0.7; 3.2; need to maintain, g / kg 0.75: 0.41; 0.14; 0.31; 0.16; 0.35; 0.60; 0.44; 0.50; 0.11; 0.53. In addition, the article presents the norms of amino acids per 1 kg of dry matter (DM) ration. However, the proposed standards need a comprehensive assessment in the research and practice of dairy farming. Research is needed to improve the models and predict the MEAA in the rations in the following areas: studying the metabolism of amino acids in the body of cows and determining their costs for maintaining the digestive tract, tissues and organs, refining, on this basis, the utilization of amino acids for milk protein production and maintenance; the development of ideal amino-acid profile of low-protein diets, both due to the adjustment of natural ingredients, and through the use of amino acid preparations that are protected from disintegration in the rumen