name
Коnоnеnко Sergey Ivanovich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
• Kuban State Agrarian University
• North-Caucasus Research Institute of Animal
кафедра физиологии и кормления сельскохозяйственных животных
профессор
Research interests
кормление животных, кормовые добавки, ферменты, комбикорма, биологически активные вещества
Web site url
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Current rating (overall rating of articles)
0
TOP5 co-authors
Articles count: 32
Сформировать список работ, опубликованных в Научном журнале КубГАУ
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ISOLATION AND SELECTION OF THE NON TRANSDUCING E. COLI BACTERIOPHAGES FOR ANTI-COLIBACILLOSIS DRUGS
DescriptionThe isolation of E.coli phages from samples of natural and waste water obtained during expeditions in the different regions of Russian Federation was carried out. The obtained phages (286 isolates) were tested for their ability to lyse the pathogenic strains of E. coli – pathogenic agents of pig colibacteriosis in Krasnodar region. The study was conducted of their ability to phage transduction, the molecular-genetic characterization and biotechnological parameters of selected bacteriophages. For first experimental design of veterinary drugs was selected 5 coliphages having no ability of plasmids transduction. It has been shown that all the investigated phages are representatives of T4-type phages of family Myoviridae. The reported study was partially supported by RFBR, research projects No. 08-04-99111, 09-04-10132, 16-44- 230855
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ACTUAL PROBLEMS IN ORGANIZATION OF FEEDING IN MODERN CONDITIONS
06.00.00 Agricultural sciences
DescriptionModern pig farming is a leading global livestock industry, but the organization of adequate balanced feeding is actual for the industry. Digestibility of metabolizable energy of feed depends on various factors, including the content of non-starch polysaccharides, which have a negative effect on the digestibility of key nutrients of the feed, lead to a decrease in growth rate and conversion efficiency of nutrients. Enzyme preparations which composition generally includes Xylanase, cellulase, β-glucanase activities, may avoid the negative impact of non-starch polysaccharides. Today's promising preparation that combines the functions of two feed additives - feed enzyme and probiotic, is an enzyme preparation Cellobacterin that, due to the particular organization of the enzyme complex, increases the digestibility of barley grain and effectively effects on sunflower meal. As a probiotic preparation it inhibits the development of pathogenic microorganisms and promotes the formation of beneficial microflora in the digestive tract. The introduction of Cellobacterin enzyme preparation into the compound feed with a high content of barley grain and sunflower meal increases the body weight of young pigs by 4.8% and reduces the cost of feed per 1 kg of live weight gain. The results of hematological studies found no significant deviations from the physiological norm in the morphological picture of the blood in experimental animals. The group, where Cellobacterin was added, showed higher vitamin A content by 2.8% as compared to the control, while vitamin E is found in both groups on the same level. Based on the results of the research to improve the productivity and growth rate in compound feed based on barley and sunflower meal with higher fiber content, it is recommended to include in the compound feed Cellobacterin enzyme preparation at the rate of 1 kg per 1 ton of feed
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PROBLEMS AND PROSPECTS OF TRITICALE IN FEEDING
06.00.00 Agricultural sciences
DescriptionIn the decision to establish solid fodder resources in the arid parts of Southern Russia triticale should take its rightful place. It must be introduced instead of winter wheat, inferior to the yield, quality and output of nutrients per unit of area. It is necessary to maximize the potential of new varieties of winter triticale, as the productivity of animals largely depends on the varietal characteristics of the culture. Triticale has attracted special attention by its ability to exceed the parental forms on productivity and quality, and resistance to adverse soil and climatic conditions and the most dangerous disease is not inferior to rye. The generalized data of most researchers characterize triticale as a factor in increasing the productivity of animals and reducing the cost of feed per unit of output, although in some cases there is a negative trend in reduction of productivity by the replacement of cereal grain components for winter triticale. Due to the enrichment of compound feed with triticale grain by enzyme preparations and enzyme-probiotic supplements we can greatly increase productivity, digestibility of main nutrients, improve meat quality and reduce the cost of feed per unit of output. We can increase the productive effect of triticale grain by extrusion. In physiological metabolic experiments, it is found that by extruding of triticale grain the digestibility of the main nutrients is increased by 1.3-1.8%. Extruding of triticale before feeding promotes slaughter yield and increases the levels of protein in the muscle. The use of triticale in rations instead of wheat reduces the cost of the diet, the cost of the manufactured products and increases profitability. The study of achievements of scientists from various scientific organizations necessitates the further breeding work with the culture of triticale and requires the development of innovative technological methods that can help reduce the concentration of pentosans and beta-glucans in the grain
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06.00.00 Agricultural sciences
DescriptionThe article presents materials on comparative study of the nutritional value of chickpeas and peas which are widespread in the arid zone of the Southern Urals. The scheme of the scientific and economic experiments involved the assessment of yield formation of chickpeas and peas during the vegetation. The study showed that different varieties of chickpea and pea have considerable difference in the content of nutrients. The amount of crude protein in the pea grain ranged from 25.1 to 26.8%, and chickpea – 21.7 - 22.9%. With regard to the fat, its highest concentration was in chickpea grain - 3.63%, on average, which is 1.16% higher than on average in the pea grain. The chemical composition of the pea and chickpea grain of different varieties points to differences in their composition and homogeneity within the culture. Basing on the study, we recommend to sow chickpea and pea grain in the middle of May to obtain the highest yield, this, in turn, has a positive effect on the nutritional value of the crops. When selecting plants for cultivation in the conditions of the Southern Urals, it is preferable to choose the variety of Madonna pea, which has a higher potential for yield than the chickpea variety Krasnokutsky-123. It has been experimentally found that chickpea grain has better moisture-retaining power in a bound condition during the vegetation period as compared to the relevant characteristics of pea grain, which is a positive drought-resistant value of crop. In structural elements of the harvest the peas showed the trend of the highest rates in comparison with the corresponding elements in chickpeas
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EFFECT OF FAT ADDITIVE ON THE PRODUCTIVITY
06.00.00 Agricultural sciences
DescriptionThe author has studied the efficiency of diets with kiselgura. Introduction of the kiselgura into the mixed fodder for chicken-broilers favors increasing of live weight by 4.8 % and average daily weight gain by 5.0%
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CONSTRUCTION OF MUTANTS OF BACTERIOPHAGE T4 WITH REDUCED ANTIGENICITY
DescriptionA method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
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MIXED FODDERS WITH RAPESEED OILCAKE FOR PIGS
06.00.00 Agricultural sciences
DescriptionThe efficiency of replacement of sunflower oilcake by rapeseed oilcake from double-zero rapeseed varieties was proved for mixed fodders for pigs
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06.00.00 Agricultural sciences
DescriptionZoo engineering expediency of triticale utilization as part of combined fodder for pigs in their growing and finishing is studied. Triticale grain may be used as part of combined fodder for young pigs up to 50 %
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FEEDING EFFICIENCY OF MULTIENZYMATIC AGENT AS A COMPONENT FOR COMBINED FEEDS
06.00.00 Agricultural sciences
DescriptionApplication of enzymatic agent MEK CX-3 (as a component of combined feeds for broiler chicks) promotes live weight gain, feed conversion efficiency and safety of young birds
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THE OUTLOOK FOR SORGHUM IN ANIMAL HUSBANDRY
06.00.00 Agricultural sciences
DescriptionThe article gives theoretical and practical justification of sorghum use in animal feeding. The results of the study of organization of forage resources for livestock and poultry with the use of forage sorghum are shown. The ways and directions for the increase of nutritive value and productive effect in the diets for animals and poultry are determined