name
Коnоnеnко Sergey Ivanovich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
• Kuban State Agrarian University
• North-Caucasus Research Institute of Animal
кафедра физиологии и кормления сельскохозяйственных животных
профессор
Research interests
кормление животных, кормовые добавки, ферменты, комбикорма, биологически активные вещества
Web site url
—
Current rating (overall rating of articles)
0
TOP5 co-authors
Articles count: 32
Сформировать список работ, опубликованных в Научном журнале КубГАУ
-
DESIGN OF THERAPEUTIC PHAGE COCKTAILS BASED ON T4-TYPE BACTERIOPHAGES: ADVANTAGES AND DISADVANTAGES
DescriptionIn the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy
-
CONSTRUCTION OF MUTANTS OF BACTERIOPHAGE T4 WITH REDUCED ANTIGENICITY
DescriptionA method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
-
06.00.00 Agricultural sciences
DescriptionStudied the investigation of the therapeutic effect of experimental mixture of four non-transducing E. coli bacteriophages and it combinations with probiotic, designed on the basis of the endemic Lactobacillus paracasei strain, previously isolated from pigs in this age of the local population in the state, the survival rate and laboratory parameters of pigs with post-weaning diarrhea. The most influence on the correction of E. coli dynamics has a combined oral administration of phage medicine and probiotic preparation established. The most influence on the dynamics of titer phages has not only the combined using (both oral and intradermal) of phage medicine with the probiotic preparation, but also an isolated ingestion of phage mixture
-
MIXED FODDERS WITH RAPESEED OILCAKE FOR PIGS
06.00.00 Agricultural sciences
DescriptionThe efficiency of replacement of sunflower oilcake by rapeseed oilcake from double-zero rapeseed varieties was proved for mixed fodders for pigs
-
THE APPLICATION OF THE BACTERIOPHAGES TO COMBAT COLIBACILLOSIS AND CAMPYLOBACTERIOSIS IN POULTRY
DescriptionThis work considers the needs and methods of phage therapy of two bacterial infections in poultry. The review examines the use of bacteriophages, from the moment of their discovery to the present day. We discuss the relevance of such diseases as compilobacteriosis and colibacteriosis transmitted from birds to humans, we show how to use bacteriophages and their efficacy in models of adult birds and chickens, including information provided on the pilot study of phage therapy at a poultry farm. The reported study was partially supported by RFBR, research projects No. 16-44-230855 and No. 13-04- 00991 and № 16-44-230855-р_а
-
HEMATOLOGICAL PARAMETERS OF PIGS WHEN USING YAKON TUBERS AND ADSORBENTS IN RATIONS
06.00.00 Agricultural sciences
DescriptionUnder the current conditions, it is very important to boost the production of pork and improve its sanitary and hygienic quality for the successful handling of problems subjected to import substitution of meat products. This can be reached by means of strengthening of food reserve. For that purposes, in the frame of two experiments yacon tubers in combination with enzymatic agent protosubtilin GZx have been introduced in the intakes of fattening pork stores instead of fodder beat, and various doses of carbitox adsorbent have been additionally added when superfluous background of heavy metals was observed. During the 1st experiment, the substitution of fodder beet with the similar quantity of yacon tubers has allowed the animals of experimental group to outweigh the ones from the control group on 7,5% in the terms of gross formation of body weight, and to gain 100 kg of body weight for 7 days ahead of the animals from control group as well. Additionally, fodder costs per product have been reduced and the morphological and biochemical values of animals blood have been optimized. Based on the data from 2 nd experiment, it has been defined that the better dose for feeding of adsorbent carbitox was 2,0 kg per tonne of concentrates thereby the animals of 3rd experimental group had reasonably higher body weight gain and thereby higher feed-conversion ratio by products. Therewith, the animals intermediary metabolism has been speeded up due to optimization of morphological and biochemical values of blood and reduction of heavy metals content in blood serum, in other words level of zinc, lead and cadmium was not exceeding maximum allowable concentration
-
ISOLATION AND SELECTION OF THE NON TRANSDUCING E. COLI BACTERIOPHAGES FOR ANTI-COLIBACILLOSIS DRUGS
DescriptionThe isolation of E.coli phages from samples of natural and waste water obtained during expeditions in the different regions of Russian Federation was carried out. The obtained phages (286 isolates) were tested for their ability to lyse the pathogenic strains of E. coli – pathogenic agents of pig colibacteriosis in Krasnodar region. The study was conducted of their ability to phage transduction, the molecular-genetic characterization and biotechnological parameters of selected bacteriophages. For first experimental design of veterinary drugs was selected 5 coliphages having no ability of plasmids transduction. It has been shown that all the investigated phages are representatives of T4-type phages of family Myoviridae. The reported study was partially supported by RFBR, research projects No. 08-04-99111, 09-04-10132, 16-44- 230855
-
EFFECT OF FERMENTS ON PRODUCTIVITY
06.00.00 Agricultural sciences
DescriptionThe author has studied the efficiency of diets with NATUPHOS 5000 enzyme preparation. Adding the enzyme preparation into the mixed fodder for chicken broilers favors increase of live weight by 4,5 % and average daily weight gain by 6,1 %
-
EFFEKT OF PROTEIN ADDITIVES FEEDING ON PRODUCTIVITY
06.00.00 Agricultural sciences
DescriptionTo solve the problems of full-value feeding for pigs, it is offered to use protein-vitamin-mineral additives as a component of rations. It allows increasing live weight gain, average live weight gain and improving feed conversion efficiency per 1kg of live weight gain
-
EFFECT OF FAT ADDITIVE ON THE PRODUCTIVITY
06.00.00 Agricultural sciences
DescriptionThe author has studied the efficiency of diets with kiselgura. Introduction of the kiselgura into the mixed fodder for chicken-broilers favors increasing of live weight by 4.8 % and average daily weight gain by 5.0%