Institut of Vine and Wine “Magarach”
Author list of organization
List of articles written by the authors of the organization
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PHENOTYPING OF NEWEST TABLE GRAPE VARIETIES AND FORMS, SELECTION OF NIVIV "MAGARACH" AND KUBSAU
06.00.00 Agricultural sciences
DescriptionIn presence article results of the three-year study of new varieties of table grapes and elite forms of super-early and very early ripening by their Agrobiological and economically valuable characteristics in terms of the Crimea and North Caucasus.
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CROSSABILITY OF CRIMEAN INDIGENOUS GRAPE VARIETIES WITH FORMS OF VARIOUS ORIGIN
06.00.00 Agricultural sciences
DescriptionProgress of breeding for resistance to diseases, pests and low temperatures is provided by cross-species hybridization. Institute of Vine and Wine "Magarach" is one of the leading centers of selection of grapes in the world. The breeding program of deducing grapes at the institute is based on the study and attracting the world's gene pool, including and industrial of the Russian Federation assortment of grapes. Analysis assortment shows that the share of productive, highquality varieties with group resistance to diseases, pests and extreme environmental factors is very small. The main method of selection of grapes of a new generation who have genetically determined traits earliness, resistance to biotic and abiotic factors is crossing complex interspecific hybrids and forms of their involvement with each other and with the forms of species Vitis vinifera, belonging to different ecogeographical groups, saturating crossing between hybrid forms with complex features, the appropriate selection of reference. For vigorous offspring is most effective as a parent to use a local variety of forms of Crohn and father - interspecific cultivar Alminsky
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06.00.00 Agricultural sciences
DescriptionEffects of different levels of vitamins (thiamine, pyridoxine and nicotinic acid) and macro-elements (MS, NN and PG) on development of globular, heart- and torpedo-stage embryos and plantlets with green cotyledons and on shoot production from them were studied in grapevine interspecific hybrids: cvs. ‘Bianca’, ‘Podarok Magaracha’ and ‘Intervitis Magaracha’. Pro-embryogenic calli were derived from petiole explants on solid full-strength NN medium supplemented with different levels of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (BA) for each cultivar and used to initiate cell suspensions in liquid NN medium supplemented with 1 mg L-1 2,4-D and 0.2 mg L-1 BA. The following formulations of media were optimal for different stages of somatic embryogenesis and for plantlets with green cotyledons development: liquid NN medium supplemented with 0.5 mg L-1 BA for globular embryo formation; liquid HTE medium (with PG macro-elements, thiamine and pyridoxine at 5 mg L-1 each and 0.5 mg L-1 nicotinic acid): supplemented with 0.2 mg L-1 BA for heart-stage embryo development, supplemented with 0.1 mg L-1 indole-3-acetic acid (IAA) and 30 mg L-1 sodium humate for torpedo-stage embryo development and supplemented with 0.5 mg L-1 gibberellic acid (GA3) for the growth of plantlets with green cotyledons. Solid MS medium modified by the addition of nicotinic acid, pyridoxine and p-aminobenzoic acid (PAB) at 5 mg L-1 each, 0.5 mg L-1 thiamine and 0.5 mg L-1 BA was best efficient for shoot production from plantlets. Inflorescence formation in regenerated plants of cv. ‘Podarok Magaracha’ grown on solid hormone-free PG medium was induced by addition of nicotinic acid, pyridoxine and PAB at 5 mg L-1 each, 0.5 mg L-1 thiamine and 0.2 mg L-1 BA into liquid modified HTE medium for growth of plantlets with green cotyledons and into solid modified MS medium for shoot production from them.
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DEVELOPMENT OF SIGNIFICANT PHENOTYPICAL TRAIT MODELS OF TABLE GRAPE
DescriptionIntensive viticulture as a sub-branch of the grape-wine industry of agriculture is developing due to the introduction of modern scientific developments. The intensification of production consists of laying vineyards with planting material of higher biological categories, highly productive clones of traditional and long-established selection varieties; advanced agrotechnological techniques and mechanized operations, etc. For import substitution of table grapevine in our country, it is especially important to introduce new big-berry varieties from early to late maturity, providing a continuous 100-day conveyor for harvesting, with a bunch of grapes, various colors and the shape of berries. In many countries of the world with developed viticulture, breeding programs are being conducted to create competitive table varieties. As a result, there were obtained Muscat of Italy (Italy), Cardinal, Red Globe (USA), Moldova (Moldova), widely spread all over the world. But the world market does not stand still and breeders are working on new varieties. In recent years, there has been a trend of enlargement of berries from 8 to 24 grams, preference is given to varieties with pink and red berries. So, for example, created in Ukraine - a very early variety of Livia; in Russia - the variety clones of Livia K and Rochefort K, the early varieties of the Memory of the Teacher, the Gift of the Nesvetay, the Preobraghenie and the Jubilee of Novocherkassk, and others. These varieties are bred according to the developed model, which shows the genetic regularities of the formation of maximum expression in transgressive recombinants in quantitative (mass of berries and bunches) and qualitative (shape and color of berries) traits associated with maturation
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06.00.00 Agricultural sciences
DescriptionTwo methods were developed, one to assess experimental results by reducing a number of developmental parameters to the overall quality, leading to the overall quality criterion, and the other to optimize processes affected by numerous interacting factors, in-vitro plant development in this case, by applying a mathematical design of experiment. Single-bud cuttings with one leaf of two Vitis vinifera L. genotypes were excised from the central part of two-month-old in-vitro grown plants and used as explants. The explants were established on bridges of filtering paper in liquid media and on solid media. Eighteen modifications of these media contained five macro-elements, each at three concentrations, and the distribution of these macro-element concentrations followed the law of random numbers (Experiment I). Parameters characterizing arm, leaf and root development of two-month-old plants of each study genotype established on each liquid or solid medium were reduced to the overall quality of plant development. Since the study genotypes differed in the ability to grow on liquid and solid media with different macro-element concentrations and also in the ability to utilize these macro-elements, the dependence of in-vitro plant development on macro-element concentrations was described by different regression equations. That is why the regression equation describing the average outcome of plant development in the two genotypes on liquid and solid media was not as significant and the description of the process was not as adequate (determined) as regression equations calculated for each process. The regression equation which describes the dependence of the average outcome of plant development on macro-element concentrations in Experiment I is as follows: y5 = 0.027 + 0.116x22 + 0.109x2x4 + 0.106x2x3 + 0.114x4. Stepwise calculation of macro-element concentrations to optimize in-vitro plant development was done based on macro-element concentrations of the initial medium of Experiment I as starting points since that medium was best efficient for this purpose by using regression equation (y5) and algorithms of multiple curvilinear stepwise regression according to the Box-Wilson method of steepest ascent. Experiment II was undertaken where macro-element concentrations (‘steps’) were calculated in a stepwise manner to optimize in-vitro plant development of the two V. vinifera genotypes and the rootstock ‘Kober 5BB’. This led to a liquid medium and a solid one which enabled a better plant development in the three genotypes relative to the use of controls: media with ½ MS macro-elements and the initial medium whose macro-element concentrations entered as starting points to calculate ‘steps’ for optimization of in-vitro plant development. The optimized medium contained macro-elements: 318 mg l-1 NH4NO3 (x1), 1188 mg l-1 KNO3 (x2), 370 mg l-1 MgSO4 7H20 (x3) (MS), 370 mg l-1 KH2PO4 (x4), 331 mg l-1 CaCl2 (x5) (MS), and other substances at optimum concentrations adjusted earlier: ¼ MS Fe-EDTA, ¼ MS micro-elements, 20 mg l-1 myo-inositol, 0.1 mg l-1 thiamine (MS), 0.5 mg l-1 nicotinic acid (MS), 0.2 mg l-1 pyridoxine, 2 mg l-1 glycine (MS), 0.1 mg l-1 indole-3-acetic acid, 10 g l-1 sucrose and, only for solid media, 7 g l-1 Difco agar. The optimized medium may be used for propagation of virus-free plants, valuable clones and grapevine genotypes created by gene engineering. The mathematical design of experiment reported in this paper which enables stepwise optimization of in-vitro plant development may be used both in agriculture and in the food industry
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06.00.00 Agricultural sciences
DescriptionNutrient media for shoot development from shoot tips 0.5-0.8 mm with several leaf primordia in four grapevine genotypes were optimized by means of a mathematical design of experiment. Over the range of component concentrations CaCl2 had considerable the highest effect on shoot tip development compared to other components of the media. Grapevine genotypes were different in their needs for concentrations of macro-elements to optimize the process of their growth and development. Shoot development from shoot tips in each grapevine genotype as a function of macro-element concentrations in media must be described by an individual regression equation. The proposed method of result evaluation and the mathematical design of experiment may be used in physiological and agricultural research for optimization of processes affected by numerous factors. The optimized media for shoot tip development may by used for sanitation of grape plants from viruses in meristem cultures
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06.00.00 Agricultural sciences
DescriptionThe influence of growth, its ripened part and the diameter of the vine at the base of the shoot on the growth power of 922 seedlings of 55 combinations of crosses in 2011 and 2012 was studied (planting scheme 3 x 1 m), seedlings of grapes of the second and third years of vegetation in the field conditions of the Southern coast of Crimea. The mean vine maturation for all populations was 64.7%, the diameter at the base of the shoots was 3.3 mm. After pruning to reverse growth, in the third year of vegetation of seedlings (2014), the length of growth of shoots increased significantly and the average for populations it became 73.3 cm. The coefficient of variation increased, 26%, - it acquired the character of a significant deviation from the general population. This happened because the seedlings, which had a vine growth less than 25 cm in the second year of vegetation, had a growth of only 35 cm in the third year, whereas in all seedlings that had a growth of more than 30 cm, in the following year shoots increased almost 1, 5 and 2 times more. Formulas are proposed for estimating the growth force of seedlings according to the 9-score scale OIV (OIV)
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DETECTED GRAPE VARIETIES OF AFGHANISTAN
06.01.01 General agriculture, crop production (agricultural sciences)
DescriptionThe article reports in color on the characteristics of the three varieties and their clones that are common in Afghanistan. In the process of ampelographic screening of vineyards in Afghanistan, three varieties previously undescribed in Russian literature under the local names Aita, Gundyan and Shandyhani were discovered. Their identification by ampelographic and ampelometric characteristics made it possible to establish the original Aita variety, the Gundyan variety as a synonym for Guladan and Shandyhani as a synonym for the world famous Sultanin, or Kishmish. The described varieties are quite common in Afghanistan, reputable in economic returns and are still promising for cultivation in their country, where they are successfully used in the manufacture of dried products and high-quality diet juices. To identify the impact of various environmental conditions on the growth, development, quantity and quality of the crop, these varieties must be tested in all zones of different ecological and geographical areas of cultivation
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06.00.00 Agricultural sciences
DescriptionPeculiarities of breeding oidium-resistant genotypes in crossing Crimea autochthonous grape varieties with complex interspecific hybrids were studied. Twentytwo populations consisting of 1323 seedlings were analyzed over a period of 2008-2014. The highest oidium resistance was registered in the progeny of the cross Khersonesskii х ЖС 26-205 (6.8 points), and the average estimate of oidium resistance across all the study populations was higher than that of the initial autochthonous grapes of the Crimea. The level of hypothetical heterosis in the majority of the study populations indicates that, on the whole, the hybrid seedlings possess a higher oidium resistance relative to the initial Crimea autochthonous grapes. The highest breeding value was associated with cross combinations having the complex interspecific hybrids Muscat JIM, Spartanets Magaracha and Magarach 31-77-10 in their pedigrees. The cross combination Muscat JIM х Shabash was the most efficient, yielding 10.7 per cent of oidium-resistant seedlings. It was reliably established that a high level of the genotypically determined inheritance of oidium resistance is found in crosses with the participation of the interspecific forms Magarach № 31-77-10 and Muscat JIM (female parents) and Spartanets Magaracha and Tsitronnyi Magaracha (male parents). The expediency of this direction of breeding research was proved
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FROST RESISTANCE OF CRIMEAN INDIGENOUS GRAPE VARIETIES AND THEIR HYBRIDS
06.00.00 Agricultural sciences
DescriptionThe article highlights the issues of grape plants resistance to low temperatures; it is an urgent problem for all wine-growing regions of the world, located in the zone of risky agriculture. Losses caused by frost, indicate long-standing necessity of perfection of assortment in terms of enhancing its hardiness by enriching more resistant genotypes obtained both through the introduction or naturalization, as well as from the crossing of indigenous with interspecies hybrids. Methodology for evaluating frost resistance properties of the material served as a preparation for the originality of his research and experimental evaluation of the unique divisibility properties of the aim. Thus, we initially conducted hardening of matured cuttings at positive temperature plus 8 plus 4 ° C for 14 days (I quenching phase) and a negative temperature minus 5 ° C-minus 7 ° C for 9 days and minus 10 ° C-1 day (II hardening phase). Then the cuttings we frozen in the temperature range from minus 16 ° C to the next steps of consistent freezing (minus 16 ° C - 2 days; minus 18 ° C - 2 days; minus 20 ° C - 2 days; minus 22 ° C – 1day, minus 24 ° C - 1 day, minus 26 ° C - 1 day, minus 28 ° C - 1 day and minus 30 ° C - 8 hours). After freezing of cuttings of each variety, the sample was placed on the 3 days in the refrigerator at the temperature of + 2 ° C to their gradual thawing. Cuttings were grown at room temperature on water. As a result, obtained by the accounting: the lowest frost-resistance among the studied forms of parental Crimean origins was found in grades of Shabash, Soldayya and Solnechnodolinsky; the varieties of Dzevat kara, Kokur bely, Kokur cherny, Misket, Misgyuli kara, Sari Pandas were set as a weak level (-20 °C); Aybatly, Kefesiya, Kok pandas and Ekim kara have average level up to -22 °C; the highest frost-resistance - 24 °C – was shown by Khersonessky and Kapselsky. Also, we have identified synthetic frost-resistant F1-population and plus-transgressive selection form